Expression of EGFR and Microvessel Density in Middle Ear Cholesteatoma

Abstract

ObjectivesCholesteatoma destructs bony tissue by the interactions between hyperproliferative epithelial cells and subepithelial inflammatory cells. The aim of this study was to evaluate the expression of epidermal growth factor receptor (EGFR) and microvessel density (MVD) in middle ear cholesteatoma tissue in an effort to determine the relationship between expression of EGFR and neovascularization.MethodsWe evaluated the expression of EGFR and MVD by immunohistochemical staining for CD31 and Factor VIII in 32 cholesteatoma tissue samples and 7 normal postauricular skin samples. We also analyzed the correlation between EGFR expression and MVD.ResultsThe expression of EGFR was higher in cholesteatoma than in postauricular skin, but the difference was not statistically significant. EGFR was more highly expressed in the suprabasal layer than in the basal layer. Using CD31 and Factor VIII, we analyzed the MVD and found that it was significantly higher in cholesteatoma than in postauricular skin, and significantly correlated with the expression of EGFR.ConclusionOur results suggest that overexpression of EGFR and neovascularization are correlated with the growth of cholesteatoma.

EGFR and microvessel density in canine malignant mammary tumours

“…The link between EGFR and MVD was studied in several human tumours (Perrotte et al, 1999;Baumann and Krause, 2004;Iivanainen et al, 2009;Diaz et al, 2010;Jin et al, 2011), including breast cancer (Fox et al, 1994;Weidner and Gasparini, 1994;Nieto et al, 2007;Nickerson et al, 2012). In CMT, MVD accessed by CD31 immunoexpression was studied by some authors (Graham and Myers, 1999;Restucci et al, 2000;Lavalle et al, 2009;Queiroga et al, 2011), and EGFR immunoexpression was also described (Gama et al, 2009;Guimarães et al, 2013;Kim et al, 2013).…”

Regulation of the Angiogenesis of Acquired Middle Ear Cholesteatomas by Inhibitor of DNA Binding Transcription Factor

“…[4][5][6][7][8][9] The most potent angiogenic factors are VEGF, IL-8, and cyclooxygenase 2 (COX-2) under inflammatory conditions in the middle ear.…”

Assessment of Subepithelial Angiogenesis in Acquired Cholesteatoma between Pediatric and Adult Patients

“…Therefore, increased CD-31 immunopositivity and the number of microvessels were accepted to be biological markers of chelesteatoma activity. Dornelles et al compared 60 pediatric and 60 adult patients in their study and they found the density of CD-31 as 7 (4)(5)(6)(7)(8)(9)(10)(11) in the pediatric patient group and as 4 (0-10) in the adult patient group (5). They evaluated this difference to be statistically significant.…”

“…In the study of Jin et al on epidermal growth factor receptor and CD-31 levels, 32 cholesteatoma tissues were compared to 6 postauricular skin tissues (6). Epidermal growth factor receptor was found to be higher in cholesteatoma tissue, although it was statistically insignificant.…”

“…Hypoxic period developing in cholesteatoma, tissue damage, and increased inflammatory cells stimulate angiogenesis through vascular and endothelial growth factors. Angiogenesis increasing in the subepithelial tissue, namely in perimatrix, can be considered as one of the biological markers of cholesteatoma activity (5,6).…”