Expression of endothelial nitric oxide synthase gene in cultured porcine granulosa cells after FSH stimulation

Takesue, Katsuhisa; Nishida, Norichika; Kato, Yosuke; Fujihara, Noború

doi:10.1677/jme.0.0260259

Cited by 19 publications

(14 citation statements)

References 37 publications

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“…Our results imply that eNOS-derived NO might also participate in the process but they might function differently. Since the expression of NOS might not be related to the synthesis of NO (Takesue et al, 2001), more direct evidence is needed to support the proposal.…”

Section: Discussionmentioning

confidence: 99%

Effects of nitric oxide synthase inhibitors on porcine oocyte meiotic maturation

Tao

Xie

Hong

et al. 2005

Zygote

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As an important biological messenger, nitric oxide (NO) exhibits a wide range of effects during physiological and pathophysiological processes, including mammalian oocyte meiotic maturation. The present study investigated whether NO derived from two nitric oxide synthase (NOS) isoforms, inducible NOS (iNOS) or endothelial NOS (eNOS), is involved in the meiotic maturation of porcine oocytes. Meanwhile, the cumulus cells' function in meiotic maturation and their interaction with oocyte development and degeneration were also investigated using cumulus-enclosed oocytes (CEOs) and denuded oocytes (DOs). Different inhibitors for NOS were supplemented to the medium. Cumulus expansion, cumulus cell DNA fragmentation and oocyte meiotic resumption were evaluated 48 h after incubation. Aminoguanidine (AG), a selective inhibitor for iNOS, suppressed cumulus expansion and inhibited CEOs to resume meiosis (p < 0.05), but did not inhibit cumulus cell DNA fragmentation. Both Nω-nitro-L-arginine (L-NNA) and Nω-nitro-L-arginine methyl ester (L-NAME), inhibitors for both iNOS and eNOS, delayed cumulus expansion, inhibited cumulus cell DNA fragmentation and inhibited CEOs to resume meiosis. Such effects were not seen in DOs. These results indicate that iNOS-derived NO is necessary for cumulus expansion and meiotic maturation by mediating the function of the surrounding cumulus cells, and eNOS-derived NO is also involved in porcine meiotic maturation.

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Section: Discussionmentioning

confidence: 99%

Effects of nitric oxide synthase inhibitors on porcine oocyte meiotic maturation

Tao

Xie

Hong

et al. 2005

Zygote

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“…The importance of NOS for meiotic maturation is confirmed by the expression of NOS isotypes in mouse and rat oocytes (eNOS, iNOSZackrisson et al, 1996;Jablonka-Shariff and Olson, 1997;Jablonka-Shariff et al, 1999;Nishikimi et al, 2001;Mitchell et al, 2004or nNOS mRNA -Abe et al, 1999 or in pig oocytes (eNOS -Hattori et al, 2000Takesue et al, 2001), and further confirmation of this comes from studies on mice with gene-knockout for eNOS. During maturation, oocytes of these mice exhibited blockage at the stage of metaphase I, or they exhibited various abnormalities (Jablonka-Shariff and Olson, 1998).…”

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confidence: 90%

The role of nitric oxide in parthenogenetic activation of pig oocytes: A review

Petr¹,

Eva²,

Tůmová³

et al. 2007

CZECH J ANIM SCI

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Parthenogenetic activation of mammalian oocytes with artificial stimuli is commonly applied in various reproductive biotechniques, e.g. cloning using nuclear transfer. For this reason, many studies focus on oocyte activation in vitro. Recently we have described the activation of pig oocytes using nitric oxide. This activating stimulus is very specific in many aspects. However, it does not provide an adequate stimulus for parthenogenetic development. It was shown that nitric oxide stimulated some signalling pathways which are inactive in conventional treatments for parthenogenetic activation, e.g. the cGMP-dependent signalling cascade. On the other hand, nitric oxide does not stimulate certain signalling pathways involved in oocyte activation after calcium ionophore, e.g. the PKC signalling cascade. The aim of this review is to characterize the complex processes induced in oocytes after treatment with nitric oxide. Perspectives for further research and the application of nitric oxide for parthenogenetic activation of oocytes are outlined.

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“…NOS isoforms also occur in the reproductive organs and oocytes of mammals (Tao et al, 1997;Jablonka-Shariff et al, 1999;Tesfaye et al, 2006). All three NOS isoforms likewise occur in pig oocytes during the growth period and meiotic maturation (Takesue et al, 2001 andChmelíková et al, 2009). The significance of NOS and NO for meiotic maturation was demonstrated in mammalian oocytes (Jablonka-Shariff and Olson, 1998;Schwarz et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Nitric oxide and meiotic competence of porcine oocytes

Tichovská

Petr

Eva

et al. 2011

Animal

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Reproductive biotechnology such as in vitro fertilization, the creation of transgenic animals or cloning by nuclear transfer depends on the use of fully grown, meiotically competent oocytes capable of completing meiotic maturation by reaching the stage of metaphase II. However, there exists only a limited quantity of these oocytes in the ovaries of females. In view of their limited number, growing oocytes without meiotic competence represent a possible source. The mechanisms controlling the acquisition of meiotic competence, however, are still not completely clear. A gas with a short half-life, nitric oxide (NO), produced by NO-synthase (NOS) enzyme can fulfill a regulatory role in this period. The objective of this study was to ascertain the role of NO in the growth phase of pig oocytes and its influence on the acquisition of meiotic competence with the help of NOS inhibitors, NO donors and their combinations. We demonstrated that the selective competitive iNOS inhibitor aminoguanidine and also the non-selective NOS inhibitor L-NAME block meiotic maturation of oocytes with partial or even full meiotic competence at the very beginning. NOS inhibitors influence even competent oocytes in the first stage of meiotic metaphase. However, blockage is less effective than at the beginning of meiotic maturation. The number of parthenogenetically activated competent oocytes greatly increased in a pure medium after inhibitor reversion. A large quantity of NO externally added to the in vitro cultivation environment disrupts the viability of oocytes. The effectiveness of the inhibitor can be reversed in oocytes by an NO donor in a very low concentration. However, the donor is not capable of pushing the oocytes farther than beyond the first stage of meiotic metaphase. The experiments confirmed the connection of NO with the growth period and the acquisition of meiotic competence. However, it is evident from the experiments that NO is not the only stimulus controlling the growth period.

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Expression of endothelial nitric oxide synthase gene in cultured porcine granulosa cells after FSH stimulation

Cited by 19 publications

References 37 publications

Effects of nitric oxide synthase inhibitors on porcine oocyte meiotic maturation

Effects of nitric oxide synthase inhibitors on porcine oocyte meiotic maturation

The role of nitric oxide in parthenogenetic activation of pig oocytes: A review

Nitric oxide and meiotic competence of porcine oocytes

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