Expression of Envelope Protein (E2) of Bovine Viral Diarrhea Virus in Insect Cells.

Kweon, Chang-Hee; Yoon, Yong-Dhuk; An, Soo-Hwan; Lee, Yong-Soon

doi:10.1292/jvms.59.415

Cited by 11 publications

(16 citation statements)

References 19 publications

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“…The arginine residue is also observed in the amino-acid sequence of membrane anchors of our recombinant E2. However, surface localisation of E2 has also been observed in cells infected with recombinant baculovirus [39] or recombinant VSV [21] that express E2. As suggested by Köhl et al [34,35] it is conceivable that, in some transfected cells, the cell retention machinery becomes saturated by overexpressed E2 glycoproteins which are then transported to the cell surface.…”

Section: Discussionmentioning

confidence: 99%

“…Neutralising antibodies and a cellular response are produced in animals vaccinated with various recombinant viruses expressing E2 [3,17,21], with DNA plasmid constructs expressing E2 [26,42,45] or with E2 subunit vaccines [7,11,12,39,58]. Purified recombinant E2 from BVDV1a strain Singer lead to partial protection against acute infection by the homologous strain [7].…”

Section: Bovine Viral Diarrhoea Virus (Bvdv)mentioning

confidence: 99%

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Sequence-optimised E2 constructs from BVDV-1b and BVDV-2 for DNA immunisation in cattle

et al. 2007

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-We report DNA immunisation experiments in cattle using plasmid constructs that encoded glycoprotein E2 from bovine viral diarrhoea virus (BVDV)-1 (E2.1) and BVDV-2 (E2.2). The coding sequences were optimised for efficient expression in mammalian cells. A modified leader peptide sequence from protein gD of BoHV1 was inserted upstream of the E2 coding sequences for efficient membrane export of the proteins. Recombinant E2 were efficiently expressed in COS7 cells and they presented the native viral epitopes as judged by differential recognition by antisera from cattle infected with BVDV-1 or BVDV-2. Inoculation of pooled plasmid DNA in young cattle elicited antibodies capable of neutralising viral strains representing the major circulating BVDV genotypes.BVDV / DNA immunisation / E2 / neutralisation / synthetic gene

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Section: Discussionmentioning

confidence: 99%

Section: Bovine Viral Diarrhoea Virus (Bvdv)mentioning

confidence: 99%

Sequence-optimised E2 constructs from BVDV-1b and BVDV-2 for DNA immunisation in cattle

et al. 2007

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“…Following transfer, the nitrocellulose membrane was blocked with TBS (0.15 M NaCl, 0.05 M Tris-HCl, pH 7.4) containing 10% skim milk for 60 min at room temperature. The membrane was then incubated for 1 hr with a 1:250 dilution of mouse sera against BVDV as described previously [12]. The membrane was washed with TBS containing 0.05% Tween 20 (TBST) and then incubated with biotin conjugated goat anti-mouse serum (KPL) for 1 hr at room temperature followed by incubation with alkaline phosphatase-conjugated streptoavidin.…”

Section: Cells and Virusmentioning

confidence: 99%

“…Previously we found that the E2 protein of BVDV expressed in baculovirus can induce neutralizing antibodies in cattle [12].…”

mentioning

confidence: 96%

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Bovine Herpes Virus Expressing Envelope Protein(E2) of Bovine Viral Diarrhea Virus As a Vaccine Candidate.

Kweon

Kang

Choi

et al. 1999

The Journal of Veterinary Medical Science

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ABSTRACT. The gene encoding the envelope protein (E2) of bovine viral diarrhea virus (BVDV) was expressed under the thymidine kinase (TK) promoter of Korean bovine herpesvirus 1 (BHV-1) isolate. Thymidine kinase negative (TK ) BHV-1 recombinants expressing E2 of BVDV were constructed and the expression of E2 was identified by immunofluorescence and Western blotting. Compared to wild type BHV-1, the recombinant BHV-1 had a delayed cytopathogenic effect in cells. The immunogenicity of the recombinant BHV-1 was examined in guinea pigs and cattle. Although an increase in body temperature was detected for a few days, the inoculated cattle returned to normal temperature with the development of neutralizing antibodies to BVDV.-KEY WORDS: BVDV, expression of E2 protein, TK BHV-1 recombinant, viral vector.

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Recombinant Bovine Adenovirus Type 3 Expressing Bovine Viral Diarrhea Virus Glycoprotein E2 Induces an Immune Response in Cotton Rats

Baxi

Deregt²,

Robertson

et al. 2000

Virology

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Recombinant bovine adenovirus is being developed as a live vector for animal vaccination and for human gene therapy. In this study, two replication-competent bovine adenovirus 3 (BAV-3) recombinants (BAV331 and BAV338) expressing bovine viral diarrhea virus (BVDV) glycoprotein E2 in the early region 3 (E3) of BAV-3 were constructed. Recombinant BAV331 contains chemically synthesized E2 gene (nucleotides modified to remove internal cryptic splice sites) under the control of BAV-3 E3/major late promoter (MLP), while recombinant BAV338 contains original E2 gene under the control of human cytomegalovirus immediate early promoter. Since E2, a class I membrane glycoprotein, does not contain its own signal peptide sequence at the 5' end, the bovine herpesvirus 1 (BHV-1) glycoprotein D signal sequence was fused in frame to the E2 open reading frame (ORF) for proper processing of the E2 glycoprotein in both the recombinant viruses. Recombinant E2 protein expressed by BAV331 and BAV338 recombinant viruses was recognized by E2-specific monoclonal antibodies as a 53-kDa protein, which also formed dimer with an apparent molecular weight of 94 kDa. Insertion of an E2-expression cassette in the E3 region did not effect the replication of recombinant BAV-3s. Intranasal immunization of cotton rats with these recombinant viruses generated E2-specific IgA and IgG responses at the mucosal surfaces and in the serum. In summary, these results show that the pestivirus glycoprotein can be expressed efficiently by BAV-3. In addition, mucosal immunization with replication-competent recombinant bovine adenovirus 3 can induce a specific immune response against the expressed antigen.

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Expression of Envelope Protein (E2) of Bovine Viral Diarrhea Virus in Insect Cells.

Cited by 11 publications

References 19 publications

Sequence-optimised E2 constructs from BVDV-1b and BVDV-2 for DNA immunisation in cattle

Sequence-optimised E2 constructs from BVDV-1b and BVDV-2 for DNA immunisation in cattle

Bovine Herpes Virus Expressing Envelope Protein(E2) of Bovine Viral Diarrhea Virus As a Vaccine Candidate.

Recombinant Bovine Adenovirus Type 3 Expressing Bovine Viral Diarrhea Virus Glycoprotein E2 Induces an Immune Response in Cotton Rats

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