Expression of Epidermal Growth Factor and Transforming Growth Factor-Alpha in Fallopian Tube Epithelium and Their Role in Embryogenesis

Kurachi, Hirohisa; Morishige, Ken‐ichirou; Imai, Takashi; Homma, Hiroaki; Masumoto, Nobuyuki; Yoshimoto, Yasuaki; Miyake, Akira

doi:10.1159/000183943

Cited by 20 publications

(7 citation statements)

References 17 publications

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“…In the negative conditioning, embryos are protected against oxidative stress and deleterious components which are regularly generated during in vitro embryo cultivation [20,34]. In the positive conditioning, embryo development is enhanced by the secretion of embryotrophic factors [35] such as leukemia inhibitory factor (LIF) [36,37], fibroblast growth factor (FGF) [38], epidermal growth factor (EGF) [39], and insulin-like growth factor (IGF) into the culture medium [35]. Our study could not provide enough evidences which mechanism have been most probably involved in the improvement of the embryo development following co-culture of embryos with either mouse or human embryonic fibroblasts.…”

Section: Discussionmentioning

confidence: 99%

The role of co-culture systems on developmental competence of preimplantation mouse embryos against pH fluctuations

Nematollahi‐Mahani

Nematollahi-mahani²,

Moshkdanian

et al. 2009

J Assist Reprod Genet

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Section: Discussionmentioning

confidence: 99%

The role of co-culture systems on developmental competence of preimplantation mouse embryos against pH fluctuations

Nematollahi‐Mahani

Nematollahi-mahani²,

Moshkdanian

et al. 2009

J Assist Reprod Genet

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“…Tubal mucosa and endometrium are sources of cytokines and growth factors which are necessary for embryos [31][32][33]. It is obvious the embryos cultured in vitro are deprived from majority of these factors.…”

Section: Discussionmentioning

confidence: 99%

Protective potential of SCF for mice preimplantation embryos cultured in vitro in suboptimal conditions

Głąbowski

Wiszniewska

Kurzawa

2008

J Assist Reprod Genet

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Purpose To examine the effect of stem cell factor (SCF) to embryos exposed to detrimental factors. Methods Mice embryos cultured in control medium or Exp.1. with FasL or FasL+SCF Exp.2. with hydrogen peroxide (HP) or HP+SCF; Exp.3. frozen-thawed and cultured with or without SCF. Immunohistochemistry for Fas and c-kit receptors was performed in blastocysts. Blastocyst rates, total numbers of blastocyst cells (TB) and inner cell mass cell counts (ICM) were determined. Results Immunohistochemical studies revealed expression of both Fas and c-kit in blastocyst cells. Exp.1. Significantly more blastocysts were found in control when compared to FasL group and to FasL+SCF group. TB and ICM counts in control and FasL+SCF group were significantly higher comparing to FasL group. Exp.2. We found significant differences between three groups in all three evaluated parameters. The highest blastocyst rates, TB and ICM counts were found in control, lower in HP+SCF group and the worst in HP group. Exp.3. No significant differences in TB and ICM counts were found. More embryos formed blastocyst in control than in two cryopreserved groups. Blastocyst rates did not differ between two cryopreserved groups. Conclusion SCF may improve culture of embryos exposed to unfavorable milieu.

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“…Immunohistochemistry using hEGF antibody was employed in this study to determine the localization of EGF in A and I oviductal regions of P and C gilts. EGF has previously been detected by means of immunohistochemical techniques in human placental tissue and human oviductal tissue [25,30,31,43]. Chegini et al [31] demonstrated the expression of mRNA and protein for EGF, TGFa, and EGF-R in human fallopian tubes.…”

Section: Discussionmentioning

confidence: 99%

“…The tubal epithelial lining was the primary binding site of these three entities, with weaker immunostaining in stromal and smooth muscle cells. Kurachi et al [43] reported that EGF and TGFa were expressed in human oviductal epithelium at specific menstrual stages, with specific immunostaining being greater in ampullary tube epithelium at late follicular and luteal stages than at the early follicular stage. Kasselberg et al [44] used immunohistochemistry and hEGF antibody to localize EGF in a variety of human maternal and fetal tissues.…”

Section: Discussionmentioning

confidence: 99%

Expression of Epidermal Growth Factor (EGF) and the EGF Receptor in the Porcine Oviduct1

Swanchara¹,

Henricks

Birrenkott

et al. 1995

Biology of Reproduction

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The production, secretion, and localization of epidermal growth factor (EGF) and the distribution of the EGF receptor (EGF-R) were examined in the isthmus (I) and ampulla (A) of the oviducts from cyclic (C) and early-pregnant (P) gilts. Sexually mature gilts (n = 20) were divided equally into two groups: C and P. P gilts were bred twice (at 0 and 24 h), and all gilts were killed 48 h after onset of estrus. After removal of reproductive tracts, oviducts were isolated, flushed, opened longitudinally, divided by anatomical region, cut into 1-3-mm3 pieces, and placed in Dulbecco's modified Eagle's Essential medium (DMEM: F-12 + ITS [insulin, 5 micrograms/ml; transferrin, 5 micrograms/ml; and selenious acid, 5 ng/ml] + antibiotic). Half the tissue and medium were immediately homogenized and centrifuged, and the supernatant was removed. The remaining tissue was cultured in the medium for 24 h at 37 degrees C and 5% CO2, then prepared similarly for analysis. EGF was measured in the supernatant by a heterologous RIA. Concentration of EGF was expressed as nanogram/milliliter of EGF per milligram of protein in wet tissue. EGF concentrations were present in both regions of the oviducts of C and P gilts. It was greater in I than in A tissues for both C (I = 16.21 ng/ml vs. A = 13.91 ng/ml; p < 0.05) and P gilts (I = 14.27 ng/ml vs. A = 12.53 ng/ml; p < 0.10). Higher concentrations of EGF were found in I tissue of C gilts than in P gilts (C = 16.21 ng/ml vs. P = 14.27 ng/ml; p < 0.05). The media assayed from cultured explants of I and A sections from C and P gilts gave results that were highly correlated with those of immediately prepared tissue sections. Localization of EGF in frozen oviductal tissue sections was demonstrated by immunohistochemistry. The primary site of EGF immunostaining occurred in the epithelial cells (with highest intensity at the apical border) of both C and P gilts. A and I tissue sections from C gilts showed localization of EGF immunostaining mainly in epithelial cells and lamina propria cells, while those from P gilts stained less intensely. The presence of EGF-R was shown by incubating tissue imprints and frozen sections with EGF-erythrosin isothiocyanate, which revealed that EGF-R were distributed mainly on the membranes of epithelial cells. The study indicates that EGF and EGF-R are present in oviductal epithelial cells in both C and P gilts, with the highest concentration of EGF in C gilts.(ABSTRACT TRUNCATED AT 250 WORDS)

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Expression of Epidermal Growth Factor and Transforming Growth Factor-Alpha in Fallopian Tube Epithelium and Their Role in Embryogenesis

Cited by 20 publications

References 17 publications

The role of co-culture systems on developmental competence of preimplantation mouse embryos against pH fluctuations

The role of co-culture systems on developmental competence of preimplantation mouse embryos against pH fluctuations

Protective potential of SCF for mice preimplantation embryos cultured in vitro in suboptimal conditions

Expression of Epidermal Growth Factor (EGF) and the EGF Receptor in the Porcine Oviduct1

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