Expression of estrogen and progesterone receptors in cytologic specimens using various fixatives

Hudock, Jude; Hanau, Cheryl A.; Christen, Randolph D.; Bibbo, Marluce

doi:10.1002/(sici)1097-0339(199607)15:1<78::aid-dc17>3.0.co;2-9

Cited by 26 publications

(27 citation statements)

References 24 publications

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“…Until the early 2000s, mainly buffered formalinfixed slides [6,7,9,12,19] were used. Some studies recommended special sequential fixation [14,21]. After the early 2000s, it was reported that alcohol fixation can be used for new antibodies [8].…”

Section: Discussionmentioning

confidence: 99%

Usefulness of liquid-based cytology in hormone receptor analysis of breast cancer specimens

et al. 2010

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Immunohistochemical (IHC) analysis of the hormone receptor (HR) in breast cancer cytology is an important issue nowadays. Several studies have shown discrepancy in the HR status between the primary tumor and metastases. Cytology can be used for patients with metastatic disease. Although cytological assessment of HR is an excellent method, it has not been routinely used because of the difficulty in consistently preparing multiple good quality slides. Liquid-based cytology (LBC) preparation is considered as the key to resolving the aforementioned problem; however, few studies have reported the HR assessment in breast cancer using LBC. Therefore, the HR status of LBC slides from 82 breast cancers was compared with that of the corresponding surgical specimens. The HR assay in both the LBC slides and surgical specimens was conducted by IHC using an autostainer. For the IHC staining, the protocol recommended by the manufacturer for paraffin-embedded sections was used for both the cytology and histology specimens. The HR results of the cytology agreed with those of the histology in 80 of the 82 cases (accuracy rate, 98%) for estrogen receptor, and in 78 of the 82 cases (accuracy rate, 95%) for progesterone receptor. The overall accuracy of the HR status on the cytology and the histology was 99% in 81 of the 82 cases. In conclusion, in HR analysis of breast cancers, LBC followed by IHC using an autostainer was useful for the standard processing of cytological specimens and showed a good correlation with the results of analysis on the histology specimens.

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Section: Discussionmentioning

confidence: 99%

Usefulness of liquid-based cytology in hormone receptor analysis of breast cancer specimens

et al. 2010

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“…[3][4][5][6][7][8] In our laboratory, ER and PR immunostains are performed on conventional smears using the same steps of fixation and processing that are used for frozen sections. Therefore, special handling at the time of the FNA and good coordination with the staff of the immunohistochemistry laboratory to ensure immediate smearing, fixing, and storing of the specimen are necessary.…”

Section: Discussionmentioning

confidence: 99%

“…studied the effects of several fixatives (including Shandon cytospin collection fluid, ethanol, and formalin) used with various preparations (such as smears and cell blocks) on the performance of ER and PR stains. 8 They reported that the smears fixed in Shandon cytospin collection fluid for ER had the largest number of positive tumor nuclei and the greatest staining intensity, whereas cell blocks fixed in formaldehyde were preferable for PR studies. Liquid-based cytology permits the collection of the FNA sample in a fixative suitable for both cytopathologic diagnosis and ancillary studies.…”

Section: Discussionmentioning

confidence: 99%

The stability of estrogen and progesterone receptor expression on breast carcinoma cells stored as preservCyt suspensions and as thinPrep slides

Tabbara

Sidawy

Frost

et al. 1998

Cancer

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“…These included the use of periodatelysineparaformaldehyde at room temperature (RT), a formalin-acetone sequence at Ϫ10°C, 11 95% alcohol, [12][13][14] 10% buffered formalin at RT, 15 acetone/formalin fixative, 16 and cytospin collection fluid, a mixture of ethanol, isopropanol, and polyoxyethylene. 17 These methods, however, have produced conflicting results. The purpose of the current study was to evaluate the effect of the most commonly used fixation methods on ER detection in cytologic smears of breast carcinoma.…”

mentioning

confidence: 99%

Optimal fixation conditions for immunocytochemical analysis of estrogen receptor in cytologic specimens of breast carcinoma

Gong

Symmans

Krishnamurthy

et al. 2003

Cancer

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Expression of estrogen and progesterone receptors in cytologic specimens using various fixatives

Cited by 26 publications

References 24 publications

Usefulness of liquid-based cytology in hormone receptor analysis of breast cancer specimens

Usefulness of liquid-based cytology in hormone receptor analysis of breast cancer specimens

The stability of estrogen and progesterone receptor expression on breast carcinoma cells stored as preservCyt suspensions and as thinPrep slides

Optimal fixation conditions for immunocytochemical analysis of estrogen receptor in cytologic specimens of breast carcinoma

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