Expression of estrogen induced gene 121-like (EIG121L) during early Xenopus development

Araki, Tetsuro; Kusakabe, Morioh; Nishida, Eisuke

doi:10.1016/j.modgep.2007.03.004

Cited by 3 publications

(12 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RT-PCR-Semi-quantitative RT-PCR and quantitative RT-PCR were performed as described previously (24). The primers for xEIG121L have been described elsewhere (24).…”

Section: Methodsmentioning

confidence: 99%

“…The primers for xEIG121L have been described elsewhere (24). The sequences of other primer pairs used were described in Table 1.…”

Section: Methodsmentioning

confidence: 99%

“…Xenopus development and found that xEIG121L mRNA is expressed in the ventral ectoderm at the gastrula and neurula stages (24). A database search revealed that xEIG121L has orthologs in other vertebrates (24).…”

Section: A Transmembrane Protein Xeig121l Is Expressed In Ventral Ectmentioning

confidence: 99%

“…A database search revealed that xEIG121L has orthologs in other vertebrates (24). Subsequent comparison of the amino acid sequences across the species raised the possibility that the previously deposited sequence of xEIG121L in the public database (Genbank TM /EMBL/DDBJ accession no.…”

Section: A Transmembrane Protein Xeig121l Is Expressed In Ventral Ectmentioning

confidence: 99%

“…We previously reported that Xenopus EIG121L (xEIG121L) mRNA has specific expression patterns during early Xenopus embryogenesis and is expressed in the ventral ectoderm at the gastrula and the neurula stages (24). EIG121L is an evolutionarily conserved gene, but its function has not been elucidated.…”

mentioning

confidence: 99%

See 4 more Smart Citations

A Transmembrane Protein EIG121L Is Required for Epidermal Differentiation during Early Embryonic Development

Araki

Kusakabe

Nishida

2011

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

Epidermal differentiation in the ventral ectoderm of Xenopus embryos is regulated by the bone morphogenetic protein (BMP) pathway. However, it remains unclear how the BMP pathway is activated and induces the epidermal fate in the ventral ectoderm. Here, we identify a novel player in the BMP pathway that is required for epidermal differentiation during Xenopus early embryonic development. We show that Xenopus EIG121L (xEIG121L) protein, an evolutionarily conserved transmembrane protein, is expressed in the ventral ectoderm at the gastrula and neurula stages. Almost complete knockdown of xEIG121L protein with antisense morpholino oligonucleotides in early Xenopus embryos results in severe developmental defects, including the inhibition of epidermal differentiation and the induction of neural genes. Remarkably, our analysis shows that BMP/Smad1 signaling is severely suppressed in the xEIG121L knockdown ectoderm. Moreover, immunoprecipitation and immunostaining experiments suggest that xEIG121L protein physically interacts, and co-localizes, with BMP receptors. Thus, our results identify a novel regulator of the BMP pathway that has a positive role in BMP signaling and plays an essential role in epidermal differentiation during early embryonic development.The bone morphogenetic protein (BMP) 4 signaling pathway has key roles in embryonic development, adult homeostasis, and diseases (1-6). A secreted dimeric ligand binds to a heterotetrameric cell surface complex of two type II and two type I kinase receptors. The type II receptor phosphorylates the type I receptor and thereby activates it. The activated type I receptor phosphorylates receptor-activated Smad (Smad1, Smad5, and Smad8 in mammals) at C-terminal serines. These phosphorylated Smad proteins form heterotrimeric complexes with the common-mediator Smad (Smad4 in mammals), and these complexes accumulate in the nucleus where they participate in the transcriptional control of target genes with sequence-specific transcription factors, co-activators, and co-repressors (1-6).In Xenopus embryos, the BMP pathway determines cell fates at the gastrula stage (7,8). In the ectoderm, the ventral region, in which the BMP pathway is activated, differentiates into epidermal tissues, and the dorsal region, in which the BMP pathway is inhibited, differentiates into neural tissues. Previous studies have shown that various factors regulate the BMP pathway during early embryonic development (7, 9 -18). Secreted factors such as Chordin, Noggin, and Follistatin bind to BMPs (BMP2, BMP4, BMP7, and anti-dorsalizing morphogenetic protein) in the extracellular space and inactivate BMP signaling at the gastrula stage, thereby inducing neural differentiation in the dorsal ectoderm (9,10,12,19). In the ventral region, secreted factors Sizzled (Szl) and Crossveinless-2 (Cv2) are induced by the BMP pathway and serve as BMP feedback inhibitors (15,17). These molecules comprise a network of BMP interacting proteins to establish the dorsoventral body axis in early embryos.In epidermal differ...

show abstract

“…RT-PCR-Semi-quantitative RT-PCR and quantitative RT-PCR were performed as described previously (24). The primers for xEIG121L have been described elsewhere (24).…”

Section: Methodsmentioning

confidence: 99%

“…The primers for xEIG121L have been described elsewhere (24). The sequences of other primer pairs used were described in Table 1.…”

Section: Methodsmentioning

confidence: 99%

Section: A Transmembrane Protein Xeig121l Is Expressed In Ventral Ectmentioning

confidence: 99%

Section: A Transmembrane Protein Xeig121l Is Expressed In Ventral Ectmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

A Transmembrane Protein EIG121L Is Required for Epidermal Differentiation during Early Embryonic Development

Araki

Kusakabe

Nishida

2011

Journal of Biological Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

A new genome scan for primary nonsyndromic vesicoureteric reflux emphasizes high genetic heterogeneity and shows linkage and association with various genes already implicated in urinary tract development

Darlow

Dobson

Darlay

et al. 2013

Molec Gen & Gen Med

View full text Add to dashboard Cite

Primary vesicoureteric reflux (VUR), the retrograde flow of urine from the bladder toward the kidneys, results from a developmental anomaly of the vesicoureteric valve mechanism, and is often associated with other urinary tract anomalies. It is the most common urological problem in children, with an estimated prevalence of 1–2%, and is a major cause of hypertension in childhood and of renal failure in childhood or adult life. We present the results of a genetic linkage and association scan using 900,000 markers. Our linkage results show a large number of suggestive linkage peaks, with different results in two groups of families, suggesting that VUR is even more genetically heterogeneous than previously imagined. The only marker achieving P < 0.02 for linkage in both groups of families is 270 kb from EMX2. In three sibships, we found recessive linkage to KHDRBS3, previously reported in a Somali family. In another family we discovered sex-reversal associated with VUR, implicating PRKX, for which there was weak support for dominant linkage in the overall data set. Several other candidate genes are suggested by our linkage or association results, and four of our linkage peaks are within copy-number variants recently found to be associated with renal hypodysplasia. Undoubtedly there are many genes related to VUR. Our study gives support to some loci suggested by earlier studies as well as suggesting new ones, and provides numerous indications for further investigations.

show abstract

Identification and characterization of Xenopus kctd15, an ectodermal gene repressed by the FGF pathway

Takahashi¹,

Suzuki²,

Nishida³

et al. 2012

Int. J. Dev. Biol.

Self Cite

View full text Add to dashboard Cite

The FGF pathway regulates a variety of developmental processes in animals through activation and/or repression of numerous target genes. Here we have identified a Xenopus homolog of potassium channel tetramerization domain containing 15 (KCTD15) as an FGF-repressed gene. Kctd15 expression is first detected at the gastrula stage and gradually increases until the tadpole stage. Whole-mount in situ hybridization reveals that the spatial expression of kctd15 is tightly regulated during early embryogenesis. While kctd15 is uniformly expressed throughout the presumptive ectoderm at the early gastrula stage, its expression becomes restricted to the non-neural ectoderm and is excluded from the neural plate at the early neurula stage. At the mid-neurula stage, kctd15 shows a more restricted distribution pattern in regions that are located at the anterior, lateral or medial edge of the neural fold, including the preplacodal ectoderm, the craniofacial neural crest and the prospective roof plate. At the tailbud stage, kctd15 expression is mainly detected in neural crest-or placode-derived tissues that are located around the eye, including the mandibular arch, trigeminal ganglia and the olfactory placode. FGF represses kctd15 expression in ectodermal explants, and the inhibition of FGF receptor with a chemical compound dramatically expands the region expressing kctd15 in whole embryos. Dorsal depletion of kctd15 in Xenopus embryos leads to bent axes with reduced head structures, defective eyes and abnormal somites, while ventral depletion causes defects in ventral and caudal morphologies. These results suggest that kctd15 is an FGF-repressed ectodermal gene required for both dorsal and ventral development.

show abstract

Expression of estrogen induced gene 121-like (EIG121L) during early Xenopus development

Cited by 3 publications

References 9 publications

A Transmembrane Protein EIG121L Is Required for Epidermal Differentiation during Early Embryonic Development

A Transmembrane Protein EIG121L Is Required for Epidermal Differentiation during Early Embryonic Development

A new genome scan for primary nonsyndromic vesicoureteric reflux emphasizes high genetic heterogeneity and shows linkage and association with various genes already implicated in urinary tract development

Identification and characterization of Xenopus kctd15, an ectodermal gene repressed by the FGF pathway

Contact Info

Product

Resources

About