Expression of exon 5 deleted estrogen receptor variant messenger RNA in human uterine myometrium and leiomyoma

Mito, Kazuko; Tamura, Takeyuki; Hosokawa, Kiyohito; Kondo, Tokumasa; Yamamoto, Takara; Honjo, Hideo

doi:10.1016/s0960-0760(98)00072-7

Cited by 8 publications

(3 citation statements)

References 42 publications

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“…For example, ER␣⌬5 has been originally detected in ER-positive progesterone receptor-negative breast cancer (5), also confirmed in several malignant tumors like uterine endometrial cancer (31), hepatocellular carcinoma (32), and meningioma (9,33). The isoform is also expressed in normal breast tissue (34) and uterus (35). An isoform of ER␣ that lacked exon 4 (ER⌬4) has been detected in rat brain (36).…”

Section: Discussionmentioning

confidence: 95%

An Estrogen Receptor β Isoform That Lacks Exon 5 Has Dominant Negative Activity on both ERα and ERβ

Inoue

Ogawa

Horie³

et al. 2000

Biochemical and Biophysical Research Communications

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Section: Discussionmentioning

confidence: 95%

An Estrogen Receptor β Isoform That Lacks Exon 5 Has Dominant Negative Activity on both ERα and ERβ

Inoue

Ogawa

Horie³

et al. 2000

Biochemical and Biophysical Research Communications

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“…The character of the smooth muscle proliferation in ferrets in the current study resembles that seen in uterine leiomyomas in women where proliferation waxes and wanes as hormone levels fluctuate. In women, however, fluctuations occur during estrogen hormone supplementation therapy following menopause (Mito et al ., 1998; Kovacs et al ., 2001), during the menstrual cycle (Kornyei et al ., 1986; Marugo et al ., 1989; Kovacs et al ., 2001; Wagner et al ., 2001) or during pregnancy (Andersen & Barbieri, 1995; Benassayag et al ., 1999; Robboy et al ., 2000). In veterinary species, endocrine disruptors such as polychlorinated biphenyls (PCBs) have been implicated in production of, and fluctuation in size of, uterine leiomyomas in seals (Backlin et al ., 2003).…”

Section: Discussionmentioning

confidence: 99%

Characterization of spindle cell component of ferret (Mustela putorius furo) adrenal cortical neoplasms – correlation to clinical parameters and prognosis

Newman

Bergman

Williams

et al. 2004

Vet Comparative Oncology

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Adrenal cortical epithelial tumours are common in ferrets. A variant tumour type with prominent spindle cell proliferation has been identified. We characterized these variant tumours with light microscopy and immunohistochemical analysis and correlate these features to clinical parameters and prognosis. We classified 24 ferret adrenal cortical masses with recognizable spindle cell proliferation obtained from the AMC and AFIP databases, based on percentage of spindle cells present and features of malignancy. These masses were separated into hyperplastic nodules and adenomas (both with 1-24% spindle cells), 'mixed' adenomas (>or=25% spindle cells), adenocarcinomas (1-24%) and 'mixed' adenocarcinomas (>or=25% spindle cells). Tumours were evaluated immunohistochemically for smooth muscle actin (SMA) and estrogen receptor (ER) expression. Disease-free interval (DFI) and survival time (ST) were calculated using Kaplan-Meier product limit method. Of 24 cases of spindle cell variant adrenal tumours, one was a hyperplastic nodule, 10 were adenomas, three were 'mixed' adenomas, six were adenocarcinomas and four were 'mixed' adenocarcinomas. The proliferative spindle cell cytoplasm was SMA-positive (smooth muscle myocyte origin). ER positivity, seen in nine of 24 cases, was restricted to adenocarcinomas, 'mixed' adenomas and 'mixed' adenocarcinomas. DFI and ST were significantly reduced in 'mixed' adenocarcinomas or tumours with ER expression. DFI was significantly reduced in tumours with marked smooth muscle. The spindle cell component of these variant adrenal tumours is smooth muscle in origin. The presence of abundant smooth muscle, a more malignant histologic grade ('mixed' adenocarcinomas) and ER expression are significantly positively correlated to both decreased DFI and decreased ST.

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“…To further substantiate results, a different approach recently described in detail [26] was also used. Briefly, the concentration of plasmid DNA, with cloned inserts of the respective gene segment of interest, was dissolved in distilled water and quantified by spectrophotometry followed by calculation of the number of copies of each plasmid DNA.…”

Section: Semiquantitative Rt-pcr Methodsmentioning

confidence: 99%

A Putative Stimulatory Role of Progesterone Acting via Progesterone Receptors in the Steroidogenic Cells of the Human Corpus Luteum1

Ottander

Hosokawa

Liu

et al. 2000

Biology of Reproduction

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To further explore the proposed auto-regulatory role of progesterone action in the human corpus luteum (CL), the expression and functional roles of progesterone receptor (PR) isoforms A and B during the luteal phase (LP) of the menstrual cycle were investigated. A total of 27 otherwise healthy patients previously scheduled for surgery were recruited after informed consent. An LH rise was detected, and CL were grouped according to age (Days 2-5 post-LH-rise, early LP; Days 6-10, mid LP; Days 11-14, late LP). Using a semiquantitative reverse transcription-polymerase chain reaction assay, the PR-B mRNA levels, which were 100- to 1000-fold lower than PR-A/B mRNA, were 46% lower (P < 0.05, n = 24) in mid LP, compared to early and late LP. CL tissue levels of progesterone and PR-A/B protein levels were inversely correlated to increasing CL age; i.e., significantly reduced levels were observed in the late LP (r(2) = 0.34, P < 0.01, n = 23). Expression of PR-A/B mRNA as well as PR-A/B protein were detected by in situ hybridization and immunohistochemistry, respectively. Both methods revealed a clear and distinct localization to cells in the steroidogenic layer of the CL. Freshly obtained mid-luteal CL cells were cultured in vitro, and media were analyzed for progesterone concentrations after treatment by incremental doses of hCG and the stable PR antagonist mifepristone, alone or in combination. Mifepristone did not per se alter progesterone synthesis, but when it was added in conjunction with hCG, a dose-related inhibitory response was seen, with a maximal 47% reduction in progesterone output at a 10 microM addition (P < 0.05, n = 3). Collectively, these data implicate a stimulatory role of progesterone receptor-mediated action in the steroidogenic cells of the human CL, which may serve as an important pathway for maintaining functional homeostasis during early pregnancy.

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Expression of exon 5 deleted estrogen receptor variant messenger RNA in human uterine myometrium and leiomyoma

Cited by 8 publications

References 42 publications

An Estrogen Receptor β Isoform That Lacks Exon 5 Has Dominant Negative Activity on both ERα and ERβ

An Estrogen Receptor β Isoform That Lacks Exon 5 Has Dominant Negative Activity on both ERα and ERβ

Characterization of spindle cell component of ferret (Mustela putorius furo) adrenal cortical neoplasms – correlation to clinical parameters and prognosis

A Putative Stimulatory Role of Progesterone Acting via Progesterone Receptors in the Steroidogenic Cells of the Human Corpus Luteum1

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