Expression of Extracellular Matrix Proteins in Human Dental Pulp Stem Cells Depends on the Donor Tooth Conditions

Miyagi, Sueli Patricia Harumi; Kerkis, Irina; Maranduba, Carlos Magno da Costa; Gomes, Cícera M.; Martins, Manoela Domingues; Marques, Márcia Martins

doi:10.1016/j.joen.2010.02.020

Cited by 23 publications

(21 citation statements)

References 19 publications

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“…These results, combined with those of a previous study showing other ECM proteins expressed by OM also found in hIDPSCs, 18 strongly suggest that dental pulp stem cells can be the precursors of OM. New studies either searching for other similarities between this neoplasm and dental pulp stem cells, or seeking to reproduce the neoplasm by inducing hIDPSCs, among other research possibilities, may contribute to clarifying the histogenesis of odontogenic tumors.…”

Section: Resultssupporting

confidence: 84%

“…All hIDPSCs expressed HA at the molecular and cellular levels. These results -along with the previous observation of other ECM molecules (e.g., type I collagen, fibronectin and tenascin) expressed by these cells and present in the tumor 18 -are indicative of at least a strong resemblance between hIDPSCs and OM cells.…”

Section: Discussionsupporting

confidence: 82%

“…18 The DL-2 cells derived from a retained permanent tooth had the lowest expression of type I collagen, whereas the DL-1 and DL-4 cells derived from erupted teeth had a significantly higher expression. 18 In this study the highest expression of MMP-2 was observed in DL-1, followed by DL-2, and the lowest was observed in DL-4. The expression of this enzyme declines with donor tooth aging.…”

Section: Discussionmentioning

confidence: 87%

“…18 These differences were observed in the expression of type I collagen and were explained based on the donor tooth conditions (deciduous or permanent, retained or erupted, and degree of root resorption). 18 The DL-2 cells derived from a retained permanent tooth had the lowest expression of type I collagen, whereas the DL-1 and DL-4 cells derived from erupted teeth had a significantly higher expression. 18 In this study the highest expression of MMP-2 was observed in DL-1, followed by DL-2, and the lowest was observed in DL-4.…”

Section: Discussionmentioning

confidence: 99%

“…16,17 Our studies have focused on the expression of ECM proteins in human immature dental pulp stem cells (hIDPCSs). 18 These cells are considered more immature than those studied by Miura et al 19 since they are able to express adult and some embryonic stem cell markers. 20 hIDPSCs are able to express type I collagen, fibronectin and tenascin, 18 all proteins present in the developing tooth 21,22 and in OM; 7 however, there are no data on the expression of HA and MMPs in dental pulp stem cells.…”

Section: Introductionmentioning

confidence: 95%

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Dental pulp stem cells express proteins involved in the local invasiveness of odontogenic myxoma

et al. 2012

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Little is known about the histogenesis of the odontogenic myxoma (OM). Dental pulp stem cells could be candidate precursors of OM because both OM and the dental pulp share the same embryological origin: the dental papilla. For the purpose of comparing OM and stem cells, this study analyzed the expression of two proteins related to OM invasiveness (MMP-2 and hyaluronic acid) in human immature dental pulp stem cells (hIDPSCs). Three lineages of hIDPSCs from deciduous and permanent teeth were used in this study. Immunofluorescence revealed positive reactions for MMP-2 and hyaluronic acid (HA) in all hIDPSCs. MMP-2 appeared as dots throughout the cytoplasm, whereas HA appeared either as diffuse and irregular dots or as short fibrils throughout the cytoplasm and outside the cell bodies. The gene expression profile of each cell lineage was evaluated using RT-PCR analysis, and HA was expressed more intensively than MMP-2. HA expression was similar among the three hIDPSCs lineages, whereas MMP-2 expression was higher in DL-1 than in the other cell lines. The expression of proteins related to OM invasiveness in hIDPSCs could indicate that OM originates from dental pulp stem cells.

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Section: Resultssupporting

confidence: 84%

Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

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Dental pulp stem cells express proteins involved in the local invasiveness of odontogenic myxoma

et al. 2012

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Modified porous scaffolds of silk fibroin with mimicked microenvironment based on decellularized pulp/fibronectin for designed performance biomaterials in maxillofacial bone defect

Sangkert

Kamonmattayakul

Chai

et al. 2017

J Biomedical Materials Res

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Maxillofacial bone defect is a critical problem for many patients. In severe cases, the patients need an operation using a biomaterial replacement. Therefore, to design performance biomaterials is a challenge for materials scientists and maxillofacial surgeons. In this research, porous silk fibroin scaffolds with mimicked microenvironment based on decellularized pulp and fibronectin were created as for bone regeneration. Silk fibroin scaffolds were fabricated by freeze-drying before modification with three different components: decellularized pulp, fibronectin, and decellularized pulp/fibronectin. The morphologies of the modified scaffolds were observed by scanning electron microscopy. Existence of the modifying components in the scaffolds was proved by the increase in weights and from the pore size measurements of the scaffolds. The modified scaffolds were seeded with MG-63 osteoblasts and cultured. Testing of the biofunctionalities included cell viability, cell proliferation, calcium content, alkaline phosphatase activity (ALP), mineralization and histological analysis. The results demonstrated that the modifying components organized themselves into aggregations of a globular structure. They were arranged themselves into clusters of aggregations with a fibril structure in the porous walls of the scaffolds. The results showed that modified scaffolds with a mimicked microenvironment of decellularized pulp/fibronectin were suitable for cell viability since the cells could attach and spread into most of the pores of the scaffold. Furthermore, the scaffolds could induce calcium synthesis, mineralization, and ALP activity. The results indicated that modified silk fibroin scaffolds with a mimicked microenvironment of decellularized pulp/fibronectin hold promise for use in tissue engineering in maxillofacial bone defects. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1624-1636, 2017.

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MSCs-Derived Decellularised Matrix: Cellular Responses and Regenerative Dentistry

Phothichailert,

Samoun,

Fournier

et al. 2024

International Dental Journal

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Expression of Extracellular Matrix Proteins in Human Dental Pulp Stem Cells Depends on the Donor Tooth Conditions

Cited by 23 publications

References 19 publications

Dental pulp stem cells express proteins involved in the local invasiveness of odontogenic myxoma

Dental pulp stem cells express proteins involved in the local invasiveness of odontogenic myxoma

Modified porous scaffolds of silk fibroin with mimicked microenvironment based on decellularized pulp/fibronectin for designed performance biomaterials in maxillofacial bone defect

MSCs-Derived Decellularised Matrix: Cellular Responses and Regenerative Dentistry

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