Expression of Fertility during Morphogenesis in Self-Pollinated Backcrossed Progenies of Barley–Wheat Amphiploids [Hordeum geniculatum All. (2n = 28) × Triticum aestivum L. (2n = 42)] (2n = 70)

Several useful alien gene transfers have been reported from related species into wheat (Triticum aestivum), but very few publications have dealt with the development of wheat/barley (Hordeum vulgare) introgression lines. An overview is given here of wheat 9 barley hybridization over the last forty years, including the development of wheat 9 barley hybrids, and of addition and translocation lines with various barley cultivars. A short summary is also given of the wheat 9 barley hybrids produced with other Hordeum species. The meiotic pairing behaviour of wheat 9 barley hybrids is presented, with special regard to the detection of wheatbarley homoeologous pairing using the molecular cytogenetic technique GISH. The effect of in vitro multiplication on the genome composition of intergeneric hybrids is discussed, and the production and characterization of the latest wheat/barley translocation lines are presented. An overview of the agronomical traits (b-glucan content, earliness, salt tolerance, sprouting resistance, etc.) of the newly developed introgression lines is given. The exploitation and possible use of wheat/barley introgression lines for the most up-to-date molecular genetic studies (transcriptome analysis, sequencing of flow-sorted chromosomes) are also discussed.

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“…(Finch and Bennett 1980), -H. geniculatum All. (Clauss 1983;Pershina et al 1988), -H. pubiflorum Hook. f. (Fedak 1983 (Jiang and Dajun 1987).…”

Section: Introductionmentioning

confidence: 99%

Wheat–barley hybridization: the last 40 years

2013

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“…Earlier in our work, amphiploid H. marinum ssp. gusso nea num-T. aestivum (2n = 70) wild barley cytoplasm was used as a maternal parent in crosses with bread wheat to obtain alloplasmic disomic wheat-barley substitution lines 7H mar (7B), 7H mar (7D), 7H mar L(7D), as well as ditelosomic addition lines 2n = 42 + 2t (7H mar L) (Pershina et al, 2004;Trubacheeva et al, 2019). In this work, individual plants of the incom plete amphiploid H. marinum ssp.…”

Section: Discussionmentioning

confidence: 99%

“…Thell. (2x, 4x) (Pershina et al, 2004;Islam et al, 2007). Due to their high resistance to salinity and waterlogging, these species are able to grow in saline meadows and marshes along sea coasts (Garthwaite et al, 2005;Islam et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Amphiploids carrying wild barley cytoplasm were produced from hybrids between H. marinum ssp. gussoneanum (4x) and T. aestivum (Pershina et al, 2004) and between H. marinum (2x) and T. aestivum (Islam et al, 2007). The salt-and waterlogging tolerances of amphiploids were intermediate to those of their parents (Islam et al, 2007;Malik et al, 2009) and reduced grain yield was observed due to the negative influence of the H. marinum cytoplasm.…”

Section: Introductionmentioning

confidence: 99%

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Development and characterization of a line with substitution of chromosome 4B of wheat Triticum aestivum L. on chromosome 4Hmar of wild barley Hordeum marinum ssp. gussoneanum (4x)

Pershina,

Trubacheeva,

Shumny

et al. 2023

Vestn. VOGiS

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Introgressive hybridization is the main method of broadening the genetic diversity of bread wheat. Wild barley Hordeum marinum ssp. gussoneanum Hudson (2n = 4x = 28) has useful agronomical traits, such as high resistance to stress factors, that could be a potential source of new genes for bread wheat improvement. This study aimed to evaluate the possibility of introgression of H. marinum chromosomes into the genome of bread wheat using an incomplete amphiploid H. marinum ssp. gussoneanum (4x)–T. aestivum (Pyrotrix 28) (2n = 54) carrying the cytoplasm of wild barley. For this purpose, we crossed the line of bread wheat variety Pyrotrix 28 with an incomplete amphiploid, and then selected cytogenetically stable 42chromosome plants with a high level of fertility in hybrid progeny. Genomic in situ hybridization (GISH) revealed a pair of H. marinum chromosomes in the genome of these plants. C banding analysis confirmed that bread wheat chromosome 4B was replaced by wild barley chromosome 4Hmar. SSR markers Xgwm368 and Xgwm6 confirmed the absence of chromosome 4B, and EST markers BAWU808 and BAW112 identified chromosome 4Hmar in the genome of the isolated disomic wheatbarley substitution line. The study of this line showed that the substitution of chromosome 4B with chromosome 4Hmar resulted in a change of some morphological traits. It included intense anthocyanin coleoptile coloration, specific for H. marinum, as well as a lack of purple coloration of the ears in the leaf sheath, specific for Pyrotrix 28. Line 4Hmar(4B) showed increased performance for several traits, including plant height, number of spikes and tillers per plant, spikelet and grain number in the main spike, grain number per plant, but it had decreased values of 1000grain weight compared to wheat. Cytogenetic stability and fertility of line 4Hmar(4B) indicated a high compensation ability of barley 4Hmar for wheat chromosome 4B and confirmed their homeology.

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“…× T. aestivum L., a great variety of selffertile recombinant alloplasmic lines were obtained among self-pollinated backcrossed progeny of hybrids and their amphiploids [12,13]. We established that the gradual replacement of barley nuclear genome with wheat genome upon backcrossing causes the introgression of barley genome fragments into the newly reconstructed wheat genome [14].…”

Section: Introductionmentioning

confidence: 99%

Study of mitochondrial genomes of allopllasmic recombinant wheat lines constructed on the basis of barley-wheat hybrids

2005

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Using RFLP analysis with three probes homologous to specific regions of mitochondrial DNA genes and PCR analysis of the mitochondrial recombining-repeat-sequence 18S/5S region of cereals, five alloplasmic wheat lines of different origin and fertility expression were studied. These lines are self-pollinated progeny of BC 1 -BC 4 generations of barley-wheat hybrids Hordeum geniculatum All. (2 n = 28) × Triticum aestivum L. (2 n = 42). It was found that recombinant alloplasmic lines characterized by partial fertility contain either maternal (barley) DNA fragments or maternal and paternal (wheat) DNA fragments simultaneously (heteroplasmy). In lines with stable expression of self-fertility, fragments of only paternal mitochondrial DNA were detected. It is assumed that in alloplasmic lines, there is the interrelation between the presence of definite fragments of the mitochondrial genome belonging to either parental type and fertility expression. PLANT GENETICS

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Expression of Fertility during Morphogenesis in Self-Pollinated Backcrossed Progenies of Barley–Wheat Amphiploids [Hordeum geniculatum All. (2n = 28) × Triticum aestivum L. (2n = 42)] (2n = 70)

Cited by 10 publications

References 4 publications

Wheat–barley hybridization: the last 40 years

Wheat–barley hybridization: the last 40 years

Development and characterization of a line with substitution of chromosome 4B of wheat <i>Triticum aestivum</i> L. on chromosome 4H<sup><i>mar</i> </sup> of wild barley <i>Hordeum marinum ssp. gussoneanum (4x)</i>

Study of mitochondrial genomes of allopllasmic recombinant wheat lines constructed on the basis of barley-wheat hybrids

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