Domestic Animal Endocrinology

2006

DOI: 10.1016/j.domaniend.2005.06.002

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Expression of foreign genes in chicks by hydrodynamics-based naked plasmid transfer in vivo

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Victoria Simchaev

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The Principle and Safety Of The Liver Targeted Gene Delivery1

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Cited by 6 publications

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“…Actually it has been calculated that ~40% of hepatocytes are transfected when a dose of 10 μg plasmid DNA is administrated into mouse tail vein (Liu et al, 1999 ). Similar distribution of expression and transfection efficiency were also observed in chickens (Hen et al, 2006 ).…”

Section: The Principle and Safety Of The Liver Targeted Gene Deliverysupporting

confidence: 76%

Technical Improvement and Application of Hydrodynamic Gene Delivery in Study of Liver Diseases

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et al. 2017

Front. Pharmacol.

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Development of an safe and efficient in vivo gene delivery method is indispensable for molecular biology research and the progress in the following gene therapy. Over the past few years, hydrodynamic gene delivery (HGD) with naked DNA has drawn increasing interest in both research and potential clinic applications due to its high efficiency and low risk in triggering immune responses and carcinogenesis in comparison to viral vectors. This method, involving intravenous injection (i.v.) of massive DNA in a short duration, gives a transient but high in vivo gene expression especially in the liver of small animals. In addition to DNA, it has also been shown to deliver other substance such as RNA, proteins, synthetic small compounds and even viruses in vivo. Given its ability to robustly mimic in vivo hepatitis B virus (HBV) production in liver, HGD has become a fundamental and important technology on HBV studies in our group and many other groups. Recently, there have been interesting reports about the applications and further improvement of this technology in other liver research. Here, we review the principle, safety, current application and development of hydrodynamic delivery in liver disease studies, and discuss its future prospects, clinical potential and challenges.

“…Actually it has been calculated that ~40% of hepatocytes are transfected when a dose of 10 μg plasmid DNA is administrated into mouse tail vein (Liu et al, 1999 ). Similar distribution of expression and transfection efficiency were also observed in chickens (Hen et al, 2006 ).…”

Section: The Principle and Safety Of The Liver Targeted Gene Deliverysupporting

confidence: 76%

Technical Improvement and Application of Hydrodynamic Gene Delivery in Study of Liver Diseases

¹

,

²

,

³

et al. 2017

Front. Pharmacol.

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Development of an safe and efficient in vivo gene delivery method is indispensable for molecular biology research and the progress in the following gene therapy. Over the past few years, hydrodynamic gene delivery (HGD) with naked DNA has drawn increasing interest in both research and potential clinic applications due to its high efficiency and low risk in triggering immune responses and carcinogenesis in comparison to viral vectors. This method, involving intravenous injection (i.v.) of massive DNA in a short duration, gives a transient but high in vivo gene expression especially in the liver of small animals. In addition to DNA, it has also been shown to deliver other substance such as RNA, proteins, synthetic small compounds and even viruses in vivo. Given its ability to robustly mimic in vivo hepatitis B virus (HBV) production in liver, HGD has become a fundamental and important technology on HBV studies in our group and many other groups. Recently, there have been interesting reports about the applications and further improvement of this technology in other liver research. Here, we review the principle, safety, current application and development of hydrodynamic delivery in liver disease studies, and discuss its future prospects, clinical potential and challenges.

“…With certain modifications, proof-of-concept experiments have been carried out in chickens 26 , rabbits 27 and pigs 28 , although, the in vivo application of this technique in larger animals remains a challenge. When using this method, another common limitation is that many of the available mammalian expression vectors lack the components to achieve a persistent, high level of gene expression.…”

Section: Introductionmentioning

confidence: 99%

Transient Expression of Proteins by Hydrodynamic Gene Delivery in Mice

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2014

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Efficient expression of transgenes in vivo is of critical importance in studying gene function and developing treatments for diseases. Over the past years, hydrodynamic gene delivery (HGD) has emerged as a simple, fast, safe and effective method for delivering transgenes into rodents. This technique relies on the force generated by the rapid injection of a large volume of physiological solution to increase the permeability of cell membranes of perfused organs and thus deliver DNA into cells. One of the main advantages of HGD is the ability to introduce transgenes into mammalian cells using naked plasmid DNA (pDNA). Introducing an exogenous gene using a plasmid is minimally laborious, highly efficient and, contrary to viral carriers, remarkably safe. HGD was initially used to deliver genes into mice, it is now used to deliver a wide range of substances, including oligonucleotides, artificial chromosomes, RNA, proteins and small molecules into mice, rats and, to a limited degree, other animals. This protocol describes HGD in mice and focuses on three key aspects of the method that are critical to performing the procedure successfully: correct insertion of the needle into the vein, the volume of injection and the speed of delivery. Examples are given to show the application of this method to the transient expression of two genes that encode secreted, primate-specific proteins, apolipoprotein L-I (APOL-I) and haptoglobinrelated protein (HPR). Video LinkThe video component of this article can be found at

“…summarizes cases where successful hydrodynamic delivery has been achieved in the liver,[2][3][4]26,28,30,31,39,[48][49][50][51][52][53][54] kidney, 27,55,56 skeletal muscle, 1,25,29,38,57-59 myocardium, 23,60 and tumor cells. 54,61 Animal species tested include fish, 55 chicken, 52 mouse, 2,3,29,35,51 rat, 1,3,27-31,38,48-50,54,58,60-62 rabbit, 25,26,63 dog, 23,29 pig, 38,39,53 monkey, 25,29 and human.…”

mentioning

confidence: 99%

Hydrodynamic Gene Delivery: Its Principles and Applications

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2007

Molecular Therapy

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Efficient and safe methods for delivering genetic materials into cells must be developed before the clinical potential of gene therapy can be fully realized. Recently, hydrodynamic gene delivery using a rapid injection of a relatively large volume of DNA solution has opened up a new avenue for gene therapy studies in vivo. This method is superior to the existing delivery systems because of its simplicity, efficiency, and versatility. Wide success in applying hydrodynamic principles to delivery of DNA, RNA, proteins, and synthetic compounds, into the cells in various tissues of small animals, has inspired the recent attempts at establishing a hydrodynamic procedure for clinical use. In this review, we provide an overview of the theory and practice of hydrodynamic gene delivery so as to aid researchers for the use of this method in their pre-clinical and translational gene therapy studies.

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