Expression of FSH receptor in the hamster ovary during perinatal development

Chakraborty, Prabuddha; Roy, Shyamal K.

doi:10.1016/j.mce.2014.11.014

Cited by 13 publications

(5 citation statements)

References 45 publications

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“…This finding indicated that M-CSF may sensitize GCs to the stimulatory effect of FSH and assist in follicular growth. Chakraborty et al (24) suggested that FSHR is expressed in the foetal hamster ovary to account for FSH-induced primordial follicle formation and cyclic AMP production. It was previously demonstrated that M-CSF facilitated the production of E 2 .…”

Section: Discussionmentioning

confidence: 99%

Molecular mechanism and functional role of macrophage colony-stimulating factor in follicular granulosa cells

Zhang

2017

Molecular Medicine Reports

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Our previous demonstrated that macrophage colony‑stimulating factor (M‑CSF) stimulated the production of estradiol (E2) and progesterone (P) in luteinized granulosa cells (GCs), and that its secretion may be regulated by follicle‑stimulating hormone (FSH). The present study aimed to examine the effect of M‑CSF alone or with Letrozole treatment on the function of non‑luteinizing granulosa cells, using the COV434 cell line, and its interaction with FSH. Human luteinized granulosa cells (LGC) were isolated from the follicular fluid of superovulated infertile patients (average age, 30.8±2.1 years) undergoing an intracytoplasmic sperm injection. The LGC were cultured with various concentrations of recombinant human macrophage colony stimulating factor (rhM‑CSF; 0, 10, 25, 50 or 100 ng/ml), rhM‑CSF+Letrozole (10‑6 mol/l), rhFSH (0, 10, 25, 50 or 100 IU/ml), or rhFSH+Letrozole (10‑6 mol/l). E2 concentrations in the media were measured using ELISA. The expression levels of the FSH receptor and the M‑CSF receptor were detected via reverse transcription‑quantitative polymerase chain reaction. Following COV434 cell treatment with M‑CSF, cell proliferation was quantified using the MTS assay and protein expression was detected by western blotting. It was demonstrated that M‑CSF and FSH stimulated the production of E2. The production of FSH receptors was enhanced by rhM‑CSF or rhM‑CSF+Letrozole in vitro in a dose‑dependent manner. It was observed that rhFSH promoted the expression of the M‑CSF receptor, at a certain concentration. Proliferation of COV434 cells increased in a dose‑dependent manner following treatment with RhM‑CSF. Furthermore, M‑CSF induced the phosphorylation of c‑Jun N‑terminal kinase (JNK) and p38; however, the level of E2 in the medium was not altered when the cells were pretreated with the JNK inhibitor SP600125 or the p38 inhibitor SB203580. The present study suggested that M‑CSF may be important in regulating the response of GCs to gonadotropin and may have a promotive effect in the early phase of follicular development. The biological effects of M‑CSF may partially be attributed to activation of the JNK and p38 signaling pathways. M‑CSF may represent a novel follicular development regulator agent in the future.

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Section: Discussionmentioning

confidence: 99%

Molecular mechanism and functional role of macrophage colony-stimulating factor in follicular granulosa cells

Zhang

2017

Molecular Medicine Reports

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“…As we all know, in the follicular phase, gonadotropins especially FSH can enhance the production of estrogen in granulosa cells (Erickson & Hsueh 1978, Jamnongjit & Hammes 2006. Besides, with the follicle development, the estrogen secretion increases and then activates the FSH receptor expression (Chakraborty & Roy 2015). We observed that, after ovary fat pad removal, the estrogen level decreased and Fshr expression was downregulated (Fig.…”

Section: Discussionmentioning

confidence: 66%

Removal of mouse ovary fat pad affects sex hormones, folliculogenesis and fertility

Wang

Cui

Zhang

et al. 2017

Journal of Endocrinology

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As a fat storage organ, adipose tissue is distributed widely all over the body and is important for energy supply, body temperature maintenance, organ protection, immune regulation and so on. In humans, both underweight and overweight women find it hard to become pregnant, which suggests that appropriate fat storage can guarantee the female reproductive capacity. In fact, a large mass of adipose tissue distributes around the reproductive system both in the male and female. However, the functions of ovary fat pad (the nearest adipose tissue to ovary) are not known. In our study, we found that the ovary fat pad-removed female mice showed decreased fertility and less ovulated mature eggs. We further identified that only a small proportion of follicles developed to antral follicle, and many follicles were blocked at the secondary follicle stage. The overall secretion levels of estrogen and FSH were lower in the whole estrus cycle (especially at proestrus); however, the LH level was higher in ovary fat pad-removed mice than that in control groups. Moreover, the estrus cycle of ovary fat pad-removed mice showed significant disorder. Besides, the expression of FSH receptor decreased, but the LH receptor increased in ovary fat pad-removed mice. These results suggest that ovary fat pad is important for mouse reproduction.

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“…Under these circumstances, the increased expression of FSHR in the POAT-deficient ovaries might be a compensatory response to restore follicle growth. The compensatory increase in FSHR was also found in FSH antiserum-treated hamsters, in which FSH antiserum markedly reduced serum FSH while Fshr mRNA was slightly increased to restore the FSH signaling in ovarian cells (Chakraborty & Roy 2015). It seemed that the compensatory increase of FSHR expression was common in the condition of FSH deficiency.…”

Section: Discussionmentioning

confidence: 82%

Peri-ovarian adipose tissue contributes to intraovarian control during folliculogenesis in mice

Yang

Chen

et al. 2018

Reproduction

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Peri-ovarian adipose tissue (POAT) is a kind of intra-abdominal white adipose tissue that is present surrounding the ovaries in rodents. Recent studies demonstrated that POAT-deficient mice displayed a phenotype of delayed antral follicular development, for which decreases in serum estrogen, serum FSH and FSHR levels were responsible. However, folliculogenesis is regulated by endocrine signals and also modulated by a number of locally produced intraovarian factors whose acts are both autocrine and paracrine. Here, we used a model of surgical removal of POAT unilaterally and contralateral ovaries as controls, as both were under the same endocrine control, to assess the paracrine effect of the POAT on folliculogenesis. Surgical removal of unilateral POAT resulted in delayed antral follicular development and the increased number of atretic follicles, accompanied by decreased levels of intraovarian adipokines and growth factors, lipid accumulation and steroidogenic enzyme expression. POAT-deficient ovaries displayed compensatory increased expressions of intraovarian genes, such as and for angiogenesis, ,, and involved in lipogenesis and in response to FSH stimulation. Furthermore, we demonstrated that removal of POAT promoted follicular apoptosis, caused retention of cytoplasmic YAP and inhibited PTEN-AKT-mTOR activation. These alterations were observed only in the POAT-deficient ovaries but not in the contralateral ovaries (with POAT), which suggests that a paracrine interaction between POAT and ovaries is important for normal folliculogenesis.

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Expression of FSH receptor in the hamster ovary during perinatal development

Cited by 13 publications

References 45 publications

Molecular mechanism and functional role of macrophage colony-stimulating factor in follicular granulosa cells

Molecular mechanism and functional role of macrophage colony-stimulating factor in follicular granulosa cells

Removal of mouse ovary fat pad affects sex hormones, folliculogenesis and fertility

Peri-ovarian adipose tissue contributes to intraovarian control during folliculogenesis in mice

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