Expression of Galectins-1 and Galectin-3 in Stomach and Colorectal Cancer with Tissue Eosinophilia

Kolobovnikova, Yu. V.; Дмитриева, А. И.; Yankovich, K I; Vasil'eva, O A; Пурлик, И. Л.; Полетика, Вадим Сергеевич; Новицкий, В. В.; Уразова, О. И.

doi:10.1007/s10517-018-4142-6

Cited by 3 publications

(2 citation statements)

References 7 publications

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“…Our study showed that blocking GAL-3 with antibodies ( Figure 5 ) reduced the pro-angiogenic ability of HUVECs and downregulated GAL-3 downstream target molecules, including VEGFR2 and JAG-1. Moreover, low expression of GAL-3 is a favorable prognostic factor in stomach and colorectal cancer [ 41 ]. We thus reasoned that BAP31 modulates angiogenesis by influencing the pVHL/HIF-1α axis, which up-regulates the expression of GAL-3 and, consequently, increases secreted GAL-3 and VEGFA and the promotion of angiogenesis by endothelial cells.…”

Section: Discussionmentioning

confidence: 99%

BAP31 Promotes Angiogenesis via Galectin-3 Upregulation in Neuroblastoma

Namusamba,

Wu,

Yang

et al. 2024

IJMS

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Neuroblastoma (NB) is one of the highly vascularized childhood solid tumors, and understanding the molecular mechanisms underlying angiogenesis in NB is crucial for developing effective therapeutic strategies. B-cell receptor-associated protein 31 (BAP31) has been implicated in tumor progression, but its role in angiogenesis remains unexplored. This study investigated BAP31 modulation of pro-angiogenic factors in SH-SY5Y NB cells. Through protein overexpression, knockdown, antibody blocking, and quantification experiments, we demonstrated that overexpression of BAP31 led to increased levels of vascular endothelial growth factor A (VEGFA) and Galectin-3 (GAL-3), which are known to promote angiogenesis. Conditioned medium derived from BAP31-overexpressing neuroblastoma cells stimulated migration and tube formation in endothelial cells, indicating its pro-angiogenic properties. Also, we demonstrated that BAP31 enhances capillary tube formation by regulating hypoxia-inducible factor 1 alpha (HIF-1α) and its downstream target, GAL-3. Furthermore, GAL-3 downstream proteins, Jagged 1 and VEGF receptor 2 (VEGFR2), were up-regulated, and blocking GAL-3 partially inhibited the BAP31-induced tube formation. These findings suggest that BAP31 promotes angiogenesis in NB by modulating GAL-3 and VEGF signaling, thereby shaping the tumor microenvironment. This study provides novel insights into the pro-angiogenic role of BAP31 in NB.

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Section: Discussionmentioning

confidence: 99%

BAP31 Promotes Angiogenesis via Galectin-3 Upregulation in Neuroblastoma

Namusamba,

Wu,

Yang

et al. 2024

IJMS

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“…Some biochemical studies for AML and control. and Malignancies) 17 . Through search in articles, we found similar results were matched with our results 18,19 .…”

Section: Discussionmentioning

confidence: 99%

Role of Vitamin D in the diagnosis of acute Myeloid Leukemia

Tahir

Thamer²,

Mahmood³

2023

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A range of hematological and biochemical markers have been investigated in Acute Myeloid Leukemia (AML) patients to determine the relationship between cancer growth and metabolic problems. This study aimed to determine the effects of vitamin D deficiency in Iraqi patients with acute myeloid leukemia who had recently been diagnosed. There was a significant inverse correlation between the total serum cholesterol (TC) level of acute myeloid leukemia (AML) patients group [(148.77±12.2) for males, (165.29±9.64) for females] and the control group [(164.50±7.26) for males, (180.05±7.31) for females], also an inverse correlation between high-density lipoprotein (HDL) level of acute myeloid leukemia (AML) patients group [(46.00±2.04) for males, (46.18±1.08) for females] and control group [(54.25±1.86) for males,(51.94±1.37) for females]. A significant difference was between the serum triglyceride (TG) level of acute myeloid leukemia (AML) patients group [(128.71±13.07) for males, (152.48±10.6) for females] and control group [85.12±11.30) for male, (90.50±10.90) for females], also between vitamin D level of acute myeloid leukemia (AML) patients group [(17.23±1.18) for males, (12.96±0.74) for females] and control group [(42.62±1.43) for males, (40.76±0.82) for females]. A statistically significant difference was between the serum calcium levels of individuals with acute myeloid leukemia [(8.99±0.32) for males, (8.91±0.23) for females] and the control group [(13.13±1.16) for males, (10.73±0.28) for females]. AML patients can benefit from vitamin D treatment, according to a pairwise analysis of receiver operating characteristic (ROC) curves. The above results are related to concluding that Vitamin D can be utilized as a diagnostic test for AML patients. Keywords; acute myeloid leukemia (AML), Hypereosinophilia, ROC curve, hypocholesterolemia, vitamin D.

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The role of galectin-1 and galectin-3 in the mechanisms of T-cell immune response dysregulation in colon cancer

et al. 2020

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Цель исследования-охарактеризовать особенности субпопуляционного состава и цитокин-секреторной активности Т-лимфоцитов (Th1, Th17 и Treg) во взаимосвязи с концентрацией галектина-1 и галектина-3 в крови у больных раком толстого кишечника. Материалы и методы. Обследованы 26 пациентов (14 мужчин и 12 женщин, средний возраст (62,9 ± 6,7) лет) с диагнозом рака толстого кишечника. В группу контроля вошли 17 здоровых доноров (11 мужчин и 6 женщин, средний возраст (58,2 ± 3,1) лет). Материалом исследования служила цельная периферическая кровь, плазма крови и супернатанты суспензионной культуры мононуклеарных лейкоцитов. Выделенные из крови лимфоциты типировали методом проточной лазерной цитофлуориметрии с использованием моноклональных антител. Методом иммуноферментного анализа определяли содержание галектина-1 и галектина-3 (в плазме крови) и IFNγ, IL-17A и TGFβ (в супернатантах культуры мононуклеарных лейкоцитов in vitro). Полученные результаты анализировали статистическими методами. Результаты. У больных раком толстого кишечника установлено значимое увеличение концентрации галектина-1 и галектина-3 в плазме крови, ассоциированное со снижением содержания CD4+T-bet+ Th1лимфоцитов, CD4+RORC2+ Th17-лимфоцитов в крови и гипосекрецией IL-17 лимфоцитами in vitro. Напротив, выявлена положительная корреляция между концентрацией галектинов 1 и 3, содержанием CD4+FoxP3+Treg клеток в крови и секрецией TGFβ мононуклеарными лейкоцитами in vitro. Заключение. При раке толстого кишечника повышенный уровень галектинов 1 и 3 в крови сопряжен с количественным дефицитом и угнетением секреторной активности эффекторных Т-лимфоцитов, и, напротив, активацией иммуносупрессорных функций регуляторных Т-клеток. Полученные результаты указывают на негативную роль галектина-1 и галектина-3 в механизмах регуляции Т-клеточного звена иммунного ответа при раке толстого кишечника.

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Expression of Galectins-1 and Galectin-3 in Stomach and Colorectal Cancer with Tissue Eosinophilia

Cited by 3 publications

References 7 publications

BAP31 Promotes Angiogenesis via Galectin-3 Upregulation in Neuroblastoma

BAP31 Promotes Angiogenesis via Galectin-3 Upregulation in Neuroblastoma

Role of Vitamin D in the diagnosis of acute Myeloid Leukemia

The role of galectin-1 and galectin-3 in the mechanisms of T-cell immune response dysregulation in colon cancer

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