Expression of gastrin-releasing peptide (GRP) in the bovine uterus during the estrous cycle

Budipitojo, Teguh; Sasaki, Motoki; Matsuzaki, Shinya; Cruzana, Maria Bella C.; Iwanaga, Toshihiko; Kitamura, Nobuo; Yamada, Junzo

doi:10.1679/aohc.66.337

Cited by 8 publications

(9 citation statements)

References 38 publications

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“…higher in UF) have previously been reported in the bovine uterus. This includes three which have been localised to GE [S100G, gastrin‐releasing peptide (GRP) and MIF] [], three which have been identified in uterine tissue (DBI, CTSD and PRDX6) [], and seven which have recently been detected in UF/fluid (RBP4, TPI, AKR1B1, HSPA8, EZR, TXN and PRDX1; where HSP stands for heat shock protein) [].…”

Section: Resultsmentioning

confidence: 99%

“…GRP (6.1‐fold higher in UF compared to plasma) has been reported to modulate ion transport across human endometrial cells in vitro and may therefore influence the intra‐uterine environment []. The presence of GRP in the bovine uterus has been reported where it is localised to the gland cells of the bovine endometrium []. Uterine GRP may also play a role in implantation as it has been reported to affect cell adhesion, morphogenesis, migration and angiogenesis [].…”

Section: Discussionmentioning

confidence: 99%

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A comparison of the bovine uterine and plasma proteome using iTRAQ proteomics

et al. 2012

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Early embryo loss is a key factor affecting fertility in dairy and beef herds. Prior to implantation, the bovine embryo spends around 16 days free-floating in the uterine environment and is dependent on the composition of uterine fluid for normal growth and development. However, there is a lack of information regarding the protein composition of the bovine uterus and how it relates to plasma. In this study, uterine flushings (UF) (n = 6) and blood plasma (n = 4) were collected from beef heifers on day 7 of the oestrous cycle, albumin depleted and compared using iTRAQ proteomics. A total of 35 proteins were higher and 18 were lower in UF including metabolic enzymes, proteins with anti-oxidant activity and those involved in modulation of the immune response. This study confirms the dynamic nature of the bovine uterine proteome and that it differs from plasma. Factors affecting the uterine proteome and how it impacts on embryo survival warrant further study.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A comparison of the bovine uterine and plasma proteome using iTRAQ proteomics

et al. 2012

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“…(2003) revealed in the cow that RT‐PCR showed GRP mRNA fragments in the tissue during all stages of the cycle. Positive staining of GRP immunoreactivity in the uterine gland epithelial cells was also detected in both the uterine horn and body during all stages of the cycle in the cow (Budipitojo et al., 2003). It is also important to show the differences of GRP immunohistochemistry in their different estrous stages in the mouse deer.…”

Section: Discussionmentioning

confidence: 96%

“…It is necessary to evaluate other Ruminantia such as Moschidae, Cervidae, Giraffidae, Antilocapridae, which have polycotyledonary synepitheliochorial placentas; the suborders such as Suiformes and Tylpoda; and other orders such as Pholidota, which has a diffuse and epitheliochorial placenta, to compare and show the immunoreactivity of GRP in their uteroplacenta. Budipitojo et al. (2003) revealed in the cow that RT‐PCR showed GRP mRNA fragments in the tissue during all stages of the cycle.…”

Section: Discussionmentioning

confidence: 98%

Immunolocalization of Gastrin‐Releasing Peptide (GRP) in the Uteroplacenta of the Mouse Deer

Kimura

Budipitojo

Sasaki

et al. 2006

Anat Histol Embryol

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The considerable phylogenetical differences between mouse deer and other ruminants have been established by means of DNA sequence analysis and anatomical observations. To clarify the physiological role of the uteroplacenta of the mouse deer, immunohistochemical observation was attempted by using GRP, which has been suggested as a novel regulatory peptide in the female reproductive tract, as an indicator to compare with other ruminants. Strong positive reactions for the GRP were detected in the uterine glands of the pregnant animals, but not in the non-pregnant ones. Although the placenta of the mouse deer is categorized as a diffuse placenta that is different from other ruminants' polycotyledonary placenta, in terms of GRP immunoreactivity, the mouse deer placenta can be classified as a synepithecholial placenta like the other ruminants'. The secretion of GRP from the uterine glands is of some importance to the fetus in the mouse deer.

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“…It was found primarily in uterine gland epithelium at 20 and 140 days of gestation. Furthermore, GRP immunoreactivity has been described in the bovine uterus and cervix (Budipitojo et al., 2001, 2003, 2004a,b) as well as the placenta (Budipitojo et al., 2001). These investigators have suggested that GRP might play an important role in the autocrine and/or paracrine regulation of the intrauterine environment of both non‐pregnant and pregnant animals (Budipitojo et al., 2001, 2004a).…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical Localization of Gastrin‐releasing Peptide, Neuronal Nitric Oxide Synthase and Neurone‐specific Enolase in the Uterus of the North American Opossum, Didelphis virginiana

Kumano

Sasaki

Budipitojo

et al. 2005

Anat Histol Embryol

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The present study has demonstrated the immunohistochemical localization of gastrin-releasing peptide (GRP), neuronal nitric oxide synthase (nNOS) and neurone-specific enolase (NSE) in the uterus of the North American opossum. Although the presence of GRP, nNOS and NSE has been reported recently in the uterus of eutherian species this is the first description of these peptides in a metatherian species. Metatherian mammals are of interest because in these species it is the prolonged lactation phase of development that is the period of primary reproductive investment rather than intrauterine development as is true of eutherian mammals. The opossum, like other marsupial species, has a very abbreviated gestation period which in Didelphis lasts only 12.5 days. GRP was localized in the cytoplasm of cells forming the surface lining epithelium and the glandular epithelium of the opossum endometrium late in pregnancy, at 11.5 days of gestation. Likewise, immunoreactivities of nNOS and NSE were found primarily within the epithelial cells of the endometrium at 11.5 days of gestation. As these peptides and enzymes appear primarily at the time of establishment of the yolk sac placenta (between day 10 and day 12.5 gestation), the present results strongly suggest that these factors may play a fundamental role in the placentation of the opossum.

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Expression of gastrin-releasing peptide (GRP) in the bovine uterus during the estrous cycle

Cited by 8 publications

References 38 publications

A comparison of the bovine uterine and plasma proteome using iTRAQ proteomics

A comparison of the bovine uterine and plasma proteome using iTRAQ proteomics

Immunolocalization of Gastrin‐Releasing Peptide (GRP) in the Uteroplacenta of the Mouse Deer

Immunohistochemical Localization of Gastrin‐releasing Peptide, Neuronal Nitric Oxide Synthase and Neurone‐specific Enolase in the Uterus of the North American Opossum, Didelphis virginiana

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