Expression of gelatinases (MMP-2 and MMP-9) and tissue inhibitors of metalloproteinases (TIMP-2 and TIMP-3) in the chicken ovary in relation to follicle development and atresia

Hrabia, Anna; Wolak, Dominika; Kwaśniewska, M; Kierońska, Anna; Socha, Joanna K.; Sechman, Andrzej

doi:10.1016/j.theriogenology.2018.11.017

Cited by 34 publications

(20 citation statements)

References 41 publications

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“…Matrix metalloprotease (MMPs) mediates basement membrane and extracellular matrix degradation and cell migration [20,21]. Moreover, MMP-9 is an important member of MMPs and is a key part in the degradation of collagen VI and other matrix proteins in basement membrane [22].…”

Section: Detection Of Related Protein Expression By Wbmentioning

confidence: 99%

Effects of Arsenic Trioxide-Loaded PLGA Nanoparticles on Proliferation and Migration of Human Vascular Smooth Muscle Cells

Zhao

Zhang

et al. 2021

Journal of Nanomaterials

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Backgroud. To evaluate improvement of arsenic trioxide-loaded PLGA nanoparticles (As2O3-PLGA-NPs) to Human Vascular Smooth Muscle Cells (HUVSMCs) in vitro. Methods. As2O3-PLGA-NPs were synthesized and characterized by transmission electron microscopy (TEM), scanning electron microscope (SEM), and energy dispersive spectrometry (EDS), and the cumulative release rates of As2O3-PLGA-NPs were measured in vitro; HUVSMCs were treated with As2O3-PLGA-NPs in vitro. MTT assay and flow cytometry assay (FCM) were performed to examine the inhibitory effect of As2O3-PLGA-NPs on HUVSMCs and compared with As2O3 solution at various concentrations. Optical microscope was used to observe the morphological change of HUVSMCs treated with As2O3-PLGA-NPs. The expression of Bcl-2, Bax, and MMP-9 in HUVSMCs was detected by RT-PCR and Western blot (WB). Results. EDS confirmed that prepared nanoparticles contained elements of arsenic. The surface coating of the eluting stent of As2O3-PLGA-NPs has the same characteristics with our self-prepared As2O3-PLGA-NPs, and it also has a drug sustained-release character. Compared with the control group, cell proliferation and migration cell were significantly suppressed with concentration-dependent ( P < 0.05 , respectively). Meanwhile, in concentration-dependent, As2O3-PLGA-NPs depressed mRNA and protein expression of Bcl-2 and MMP-9 and increased mRNA and protein expression of Bax. Conclusion. As2O3-PLGA-NPs had an inhibitory effect on HUVSMCs’ proliferation and migration, and it may work via regulating Bax, Bcl-2, and MMP-9 expression in vitro.

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Section: Detection Of Related Protein Expression By Wbmentioning

confidence: 99%

Effects of Arsenic Trioxide-Loaded PLGA Nanoparticles on Proliferation and Migration of Human Vascular Smooth Muscle Cells

Zhao

Zhang

et al. 2021

Journal of Nanomaterials

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“…In follicles of the mature hen ovary, the expression of MMP-2, MMP-3, MMP-9, MMP-10, and MMP-13 mRNAs and/or proteins as well as gelatinase (MMP-2 and MMP-9) activities increase along with follicular maturation [ 36 , 38 , 39 , 40 , 41 , 42 , 43 ]. These correlative findings suggest the engagement of some MMPs in the turnover of the ECM/follicular wall structure during the final stage of ovarian follicle maturation.…”

Section: Mmp Roles In Follicle Development and Atresiamentioning

confidence: 99%

“…These correlative findings suggest the engagement of some MMPs in the turnover of the ECM/follicular wall structure during the final stage of ovarian follicle maturation. Moreover, transcript and/or protein abundances of MMP-2, MMP-7, MMP-9, MMP-10, and MMP-13 as well as of TIMP-2 and TIMP-3 differ between white and yellowish follicles and the small yellow follicles just recruited into the preovulatory hierarchy [ 39 , 40 ]. Furthermore, atresia of white follicles is accompanied by an increase in MMP-2 and MMP-9 activities [ 39 ].…”

Section: Mmp Roles In Follicle Development and Atresiamentioning

confidence: 99%

“…Moreover, transcript and/or protein abundances of MMP-2, MMP-7, MMP-9, MMP-10, and MMP-13 as well as of TIMP-2 and TIMP-3 differ between white and yellowish follicles and the small yellow follicles just recruited into the preovulatory hierarchy [ 39 , 40 ]. Furthermore, atresia of white follicles is accompanied by an increase in MMP-2 and MMP-9 activities [ 39 ]. These observations implicate the MMP system members in determination of the prehierarchical follicle destination, i.e., follicle atresia or selection to complete development.…”

Section: Mmp Roles In Follicle Development and Atresiamentioning

confidence: 99%

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Matrix Metalloproteinases (MMPs) and Inhibitors of MMPs in the Avian Reproductive System: An Overview

Hrabia

2021

IJMS

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Many matrix metalloproteinases (MMPs) are produced in the mammalian reproductive system and participate in the regulation of its functions. In birds, the limited information available thus far indicates that MMPs are significant regulators of avian ovarian and oviductal functions, too. Some MMPs and inhibitors of MMPs are present in the hen reproductive tissues and their abundances and/or activities change according to the physiological state. The intraovarian role of MMPs likely includes the remodeling of the extracellular matrix (ECM) during folliculogenesis, follicle atresia, and postovulatory regression. In the oviduct, MMPs are also involved in ECM turnover during oviduct development and regression. This study provides a review of the current knowledge on the presence, activity, and regulation of MMPs in the female reproductive system of birds.

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“…Augmented level of MMP-9 also postulated in the PCOS pathophysiology and is also linked with progression and etiological to many other ailments such as cystic fibrosis, asthma, ulcerative colitis, cardiovascular disorders, atherosclerosis, etc. [23,24].…”

Section: Matrix Metalloproteinasesmentioning

confidence: 99%

Inflammation and Ovulation

Pant¹,

Chitme²

2021

Fibroids

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The ovulation is a complex physiological process which is very commonly affected in patients with PCOS. Understanding inflammatory process involved in ovulation is important with respect to its onset, diagnosis and treatment. There are multiple inflammatory factors are associated with ovulation however anovulation and contraception have not been therapeutically explored in context with inflammatory process. Therefore, this chapter is written to help readers to understand the basics of inflammation in ovulation and role of inflammatory mediators in ovulation. This chapter also describes genetic and molecular aspects linked to ovulation.

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Expression of gelatinases (MMP-2 and MMP-9) and tissue inhibitors of metalloproteinases (TIMP-2 and TIMP-3) in the chicken ovary in relation to follicle development and atresia

Cited by 34 publications

References 41 publications

Effects of Arsenic Trioxide-Loaded PLGA Nanoparticles on Proliferation and Migration of Human Vascular Smooth Muscle Cells

Effects of Arsenic Trioxide-Loaded PLGA Nanoparticles on Proliferation and Migration of Human Vascular Smooth Muscle Cells

Matrix Metalloproteinases (MMPs) and Inhibitors of MMPs in the Avian Reproductive System: An Overview

Inflammation and Ovulation

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