Expression of Genes Encoding Matrilin-3 and Cyclin-I During the Impairment and Recovery of Chicken Growth Plate in Tibial Dyschondroplasia

Abstract

Tibial dyschondroplasia (TD) is a skeletal disease characterized by the disruption ofendochondral bone formation in avian species. The aim of this study was to determine the expression of Matrilin-3 and Cyclin-I genes in chicken growth plate during impairment and recovery from TD. Gene expressions were analyzed by polymerase chain reaction, and proteins by immunohistochemistry and in situ hybridizations. Expression of genes encoding Matrilin-3 and Cyclin-I were diminished with parallel decrease in proteins during TD, with fewer signs of cartilage cell differentiation. In contrast, there was an increase in mRNA expressions and protein levels of both genes during the recovery phase. These findings suggest that the Matrilin-3 and Cyclin-I genes also play a role in chondrocyte differentiation during the impairment and recovery of growth plate in TD.

Tetramethylpyrazine Mitigates Toxicity and Liver Oxidative Stress in Tibial Dyschondroplasia Chickens

“…Normal broilers growth plate cartilage is circular arc with edge tidy, uniform thickness, and edge smooth (Tian et al, 2013). TD occurred on proximal growth plate cartilage cell dysplasia, avascular, non-mineralized and opaque white cartilage wedge in growth plate, eventually led to the difficulties in movement and obstacles in standing (Li and Bi, 2008;Shahzad et al, 2014a;. Current study found that normal GP exhibits regular columns and cells surrounded by blood vessels in proliferative zone (PZ) and hypertrophic zone (HZ).…”

“…It has been testified that thiram markedly prevent the GSH and SOD activity in human which leads to oxidative stress and imbalance (Marikovsky, 2002). This type of oxidative stress effects the lipid peroxidation of cell membrane and ultimately release of MDA as an end product (Perry et al, 2010;Shahzad et al, 2014a). Dalvi and Deoras (1986) reported that thiram administration to rats result in significant increase level of AST which indicated the liver damages.…”

“…After that on day 4 post hatch, chickens were distributed in 2 groups; Control (n=60) and Thiram (n=120) group. Tibial dyschondroplasia was induced by adding thiram @ 50 mg per kg of feed to thiram group ( Shahzad et al, 2014a;Shahzad et al, 2015;Iqbal et al, 2016) while control group was offered normal diet. On day 7 post hatch, after induction of TD, thiram group was equally divided into two groups designated as TD group (n=60) and TMP treatment group (n=60).…”

“…TD is characterized by avascular and non-mineralize cartilage in the tibial growth plate (Tian et al, 2013). The TD is described as abnormal differentiation of GP chondrocytes, those normally lead to cartilage vascularization, mineralization and bone formation (Pines et al, 1998;Shahzad et al, 2014a). § These authors contributed equally to this work.…”

WITHDRAWN: Evaluation of clinical, pathological and genetic indicators in chickens with thiram-induced tibial dyschondroplasia

“…Previous research has indicated that almost 30% of bone disease in chickens and turkeys is due to TD and that the disease leads to greater than 10% morbidity in China, creating serious economic losses in the poultry industry [4][5][6]. Normally, the clinical and subclinical rate of TD is 30% in chickens, which decreases disease resistance, production performance, and carcass quality and induces breast cysts, osteomyelitis, and animal welfare issues [7][8][9].…”