Expression of genes encoding proteins involved in ecdysteroidogenesis in the female mosquito, Aedes aegypti

Sieglaff, Douglas H.; Duncan, Kelli A.; Brown, Mark R.

doi:10.1016/j.ibmb.2005.01.011

Cited by 71 publications

(74 citation statements)

References 119 publications

(190 reference statements)

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“…In addition, transcript levels of the enzymes closely parallel the steroidogenic capacities of ovaries throughout the ovarian cycle, suggesting that steroidogenesis is regulated at the transcriptional level. Similar observations have been reported for the A. aegypti ovary as well as for the prothoracic glands of D. melanogaster and Bombyx mori during larval development (22,28,30).…”

supporting

confidence: 87%

“…Ecdysteroid secretion by head, thorax, gut, Malpighian tubules, or carcass of 24 h PBM females was always below or at the detection limit of the EIA, in contrast to ovaries. Our results indicate that A. gambiae ovaries are the main female steroidogenic organs that produce and secrete 20E after blood feeding, a situation similar to what has been described for Aedes aegypti (11,28,29). In addition, transcript levels of the enzymes closely parallel the steroidogenic capacities of ovaries throughout the ovarian cycle, suggesting that steroidogenesis is regulated at the transcriptional level.…”

supporting

confidence: 86%

“…Whether the situation reported here for A. gambiae could be extended to other mosquito species has yet to be demonstrated. Indeed, although adult males of A. aegypti expressed some of the genes of the ecdysteroid biosynthetic pathway, they did not seem to express the genes encoding key enzymes for producing E and 20E (28), indicating that males of this species likely do not synthesize 20E. Although 20E is known in the female mosquito to stimulate vitellogenesis during a gonotrophic cycle, its endocrine function in adult male is elusive (as described later).…”

Section: Discussionmentioning

confidence: 98%

“…1) provided the opportunity to investigate steroidogenesis in insect species of health or economic interest (21,22,(26)(27)(28). In the present study, we analyzed steroidogenesis in male and female A. gambiae during reproductive processes.…”

mentioning

confidence: 99%

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Anopheles gambiaemales produce and transfer the vitellogenic steroid hormone 20-hydroxyecdysone to females during mating

Pondeville

Maria

Jacques

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

109

127

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In female insects, the steroid hormone 20-hydroxyecdysone (20E) plays a major role in activating vitellogenesis, a process required for egg development. By contrast with vertebrates, production of large amounts of hormonal steroids has not been reported in adult male insects. In the present study, we analyzed steroidogenesis in both male and female adult of the malaria mosquito Anopheles gambiae and we found that A. gambiae male mosquitoes produce high amounts of the steroid hormone 20E. Importantly, we found that male accessory glands, but not testes, are the source of 20E. Moreover, this steroid hormone is stored in male accessory glands and delivered to females during mating. These findings suggest that male 20E may not act as a true male sex steroid, but more likely as an allohormone. Our results give new insights into speciesspecific physiological processes that govern the reproductive success of the malaria mosquito. This could thus lead to the identification of new target genes for manipulating male and/or female reproductive success, a promising way to reduce or eliminate mosquito population and therefore to control malaria transmission.allohormone ͉ ecdysteroids ͉ malaria ͉ male accessory glands ͉ mosquito

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supporting

confidence: 87%

supporting

confidence: 86%

Section: Discussionmentioning

confidence: 98%

mentioning

confidence: 99%

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Anopheles gambiaemales produce and transfer the vitellogenic steroid hormone 20-hydroxyecdysone to females during mating

Pondeville

Maria

Jacques

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

109

127

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“…A small subset of the detoxification genes has been previously described in Ae. aegypti (Sieglaff, Duncan and Brown, 2005;David et al, 2006;Lumjuan et al, 2007).…”

Section: Metabolic Resistancementioning

confidence: 99%

Abbreviations and acronyms

2018

Harmonisation of Regulatory Oversight in Biotechnology

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This work is published under the responsibility of the Secretary-General of the OECD. The opinions expressed and arguments employed herein do not necessarily reflect the official views of OECD member countries.This document, as well as any data and any map included herein, are without prejudice to the status of or sovereignty over any territory, to the delimitation of international frontiers and boundaries and to the name of any territory, city or area.Please cite this publication as: OECD (2018) The statistical data for Israel are supplied by and under the responsibility of the relevant Israeli authorities. The use of such data by the OECD is without prejudice to the status of the Golan Heights, East Jerusalem and Israeli settlements in the West Bank under the terms of international law.Photo credits: Cover © Maks Ershov.Corrigenda to OECD publications may be found on line at: www.oecd.org/about/publishing/corrigenda.htm. © OECD 2018You can copy, download or print OECD content for your own use, and you can include excerpts from OECD publications, databases and multimedia products in your own documents, presentations, blogs, websites and teaching materials, provided that suitable acknowledgment of the source and copyright owner(s) is given. All requests for public or commercial use and translation rights should be submitted to rights@oecd.org. Requests for permission to photocopy portions of this material for public or commercial use shall be addressed directly to the Copyright Clearance Center (CCC) at info@copyright.com or the Centre francais d'exploitation du droit de copie (CFC) at contact@cfcopies.com. FOREWORD │ 3SAFETY ASSESSMENTOF TRANSGENIC ORGANISMSIN THE ENVIRONMENT, VOLUME 8 © OECD 2018 ForewordModern biotechnologies are applied to plants species (crops, flowers, trees), animals and micro-organisms. The safety of the resulting transgenic organisms when released in the environment for their use in agriculture, forestry, the food and feed industry or for other applications represents a challenging issue. Genetically engineered products are rigorously assessed by their developers during their elaboration, and by governments when ready for release, to ensure high safety standards. This remains essential with new biotechnology developments using insects to fight against disease outbreaks: engineered mosquitoes need to be evaluated through a scientifically sound approach to risk/safety assessment that will inform biosafety regulators and support the decision concerning the release of these novel organisms in the environment.The OECD offers a long-standing recognised expertise in biosafety and contributes to facilitating an harmonised approach. The OECD's Working Group on Harmonisation of Regulatory Oversight in Biotechnology (WG-HROB) was established in 1995. The WG-HROB gathers national authorities responsible for the environmental risk/safety assessment of products of modern biotechnology in OECD countries and other economies. International organisations and experts involved in biosafety activities are a...

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STUDY ON ECDYSTEROID LEVELS AND GENE EXPRESSION OF ENZYMES RELATED TO ECDYSTEROID BIOSYNTHESIS IN THE LARVAL TESTIS OF Spodoptera littoralis

Iga

Blais²,

Smagghe

2012

Arch Insect Biochem Physiol

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We investigated here the ecdysteroid titers and the expression of six genes coding for known enzymes of the ecdysteroid biosynthesis in the testes of last instar larvae of the pest cotton leafworm, Spodoptera littoralis. We showed that the timing of the ecdysteroid profile was the same in testes and in hemolymph, with a small peak at day 2 and a large one at day 4 after ecdysis. Ecdysone and 20-hydroxyecdysone (20E) were detected in both tissues. 20E was the major ecdysteroid in testes and in hemolymph from day 4. Interestingly, the gene expression of the steroidogenetic enzymes, Neverland, and the five cytochrome P450 enzymes encoded by the Halloween genes was confirmed in the testes, and varied during the instar. However, from the data obtained so far, we cannot conclude that the measured ecdysteroids in the testes result from the activity of the genes under study. Indeed, it is suggested that the ecdysone produced centrally in the prothoracic glands, could have been transformed into 20E in the testes, where Sl-shade is well expressed.

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Expression of genes encoding proteins involved in ecdysteroidogenesis in the female mosquito, Aedes aegypti

Cited by 71 publications

References 119 publications

Anopheles gambiaemales produce and transfer the vitellogenic steroid hormone 20-hydroxyecdysone to females during mating

Anopheles gambiaemales produce and transfer the vitellogenic steroid hormone 20-hydroxyecdysone to females during mating

Abbreviations and acronyms

STUDY ON ECDYSTEROID LEVELS AND GENE EXPRESSION OF ENZYMES RELATED TO ECDYSTEROID BIOSYNTHESIS IN THE LARVAL TESTIS OF Spodoptera littoralis

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