Expression of genes, encoding the enzymes of cysteine metabolism in human placenta in the first and third trimesters of uncomplicated pregnancy

Korneeva, K. L.; Rodríguez, Rosalía; Ralchenko, Serhii

doi:10.15407/ubj88.01.088

Cited by 8 publications

(5 citation statements)

References 40 publications

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“…Cysteine is a non-essential amino acid required for protein production and other metabolic functions, and is important for collagen production. Cysteine metabolism is closely linked to the maintenance of redox status and protection against free radical oxidation, homocysteine elimination, and detoxification [73]. Overall, the distinctive presence of these proteins in D and LZ compared to JZ is comparable to the known function of these placental compartments in mice.…”

Section: Decidua and Labyrinth Zonementioning

confidence: 90%

Label-free differentiation of functional zones in mature mouse placenta using micro-Raman imaging

Inanc,

Bektas,

Kecoglu

et al. 2023

Preprint

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In histopathology, it is highly crucial to have chemical and structural information about tissues. Additionally, the segmentation of zones within a tissue plays an important role in investigating the functions of these regions for better diagnosis and treatment. The placenta plays an important role in embryonic and fetal development and in the diagnosis of some diseases associated with its dysfunction. This study provides a label-free approach to obtain the images of mature mouse placenta together with the chemical differences between the tissue compartments using Raman spectroscopy. To generate the Raman images, spectra of placental tissue were collected using a custom-built optical setup. The pre-processed spectra were analyzed using statistical and machine learning methods to acquire the Raman maps. We found that the placental regions called decidua and the labyrinth zone are biochemically distinct from the junctional zone. Comparison and evaluation of the Raman maps with histological images of the placental tissue were performed by a histologist and they are found to be in agreement. The results of this study show that Raman spectroscopy offers the possibility of label-free monitoring of the placental tissue from mature mice while revealing important structural information about the zones at the same time.

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Section: Decidua and Labyrinth Zonementioning

confidence: 90%

Label-free differentiation of functional zones in mature mouse placenta using micro-Raman imaging

Inanc,

Bektas,

Kecoglu

et al. 2023

Preprint

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show abstract

“…CDO and CSAD are the enzymes responsible for converting cysteine to cysteine sulfinic acid and hypotaurine for further oxidation to taurine, reactions which are considered to be major steps in the biosynthesis of taurine in mammals (Tappaz 2004;Park et al 2017). Interestingly, a recent study by Korneeva and coworkers has provided evidence of both CDO mRNA and protein expression in the human placenta (Korneeva et al 2016). CDO converts cysteine to cysteine sulfinic acid, which can either be further transaminated by aspartate amino transferase and converted to sulfur and pyruvate, or decarboxylized to hypotaurine for taurine synthesis by CSAD.…”

Section: Discussionmentioning

confidence: 99%

Placental release of taurine to both the maternal and fetal circulations in human term pregnancies

et al. 2018

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Taurine is regarded as an essential amino acid in utero, and fetal taurine supply is believed to rely solely on placental transfer from maternal plasma. Despite its potential role in intrauterine growth restriction and other developmental disturbances, human in vivo studies of taurine transfer between the maternal, placental, and fetal compartments are scarce. We studied placental transfer of taurine in uncomplicated human term pregnancies in vivo in a cross-sectional study of 179 mother-fetus pairs. During cesarean section, we obtained placental tissue and plasma from incoming and outgoing vessels on the maternal and fetal sides of the placenta. Taurine was measured by liquid chromatography-tandem mass spectrometry. We calculated paired arteriovenous differences, and measured placental expression of the taurine biosynthetic enzyme cysteine sulfinic acid decarboxylase (CSAD) with quantitative real-time polymerase chain reaction and western blot. We observed a fetal uptake (p < 0.001), an uteroplacental release (p < 0.001), and a negative placental consumption of taurine (p = 0.001), demonstrating a bilateral placental release to the maternal and fetal compartments. Increasing umbilical vein concentrations and fetal uptake was associated with the uteroplacental release to the maternal circulation (r = - 0.19, p = 0.01/r = - 0.24, p = 0.003), but not with taurine concentrations in placental tissue. CSAD-mRNA was expressed in placental tissue, suggesting a potential for placental taurine synthesis. Our observations show that the placenta has the capacity to a bilateral taurine release, indicating a fundamental role of taurine in the human placental homeostasis beyond the supply to the fetus.

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“…Additionally, identified common gene pathways involved in imprinted gene regulation of placental and fetal development are also shown. In mammals, genetic imprinting is a significant process where many genes undergoes epigenetic modification and imprinted specifically in the placenta [7][8][9][10][11][12][13][14][15][16][17][18]. Placental imprinting appears to be a continuous process that occurs throughout the pregnancy [19].…”

Section: Unique Patterns Of Gene Expression In the Placentamentioning

confidence: 99%

Imprinted Gene Regulation in the Placenta and Fetal Development

Sharifu¹,

Maingi²,

Kibaba³

et al. 2020

BJSTR

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Expression of genes, encoding the enzymes of cysteine metabolism in human placenta in the first and third trimesters of uncomplicated pregnancy

Abstract: the cellular cysteine is highly regulated in a narrow range of concentrations due to its cyto-and neurtoxicity when it is overwhelmed or its deficiency for protein synthesis and other vital metabolic reactions

Cited by 8 publications

References 40 publications

Label-free differentiation of functional zones in mature mouse placenta using micro-Raman imaging

Label-free differentiation of functional zones in mature mouse placenta using micro-Raman imaging

Placental release of taurine to both the maternal and fetal circulations in human term pregnancies

Imprinted Gene Regulation in the Placenta and Fetal Development

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