Expression of genes for B7-H3 and other T cell ligands by nasal epithelial cells during differentiation and activation

Abstract

Epithelial cells of the human respiratory tract express human leukocyte antigen (HLA) and the costimulatory molecules B7-1 and B7-2. Little is known, however, about the constitutive expression of genes encoding for the more recently identified members of the B7 homolog family of costimulatory molecules or about the influence of cellular differentiation and cytokines on their activity or on that of HLA or B7-1 and B7-2. Human nasal epithelial (HNE) cells were grown at the air-liquid interface (ALI) for 2 or 21 days to model in vivo conditions. Expression of genes for HLA-B and HLA-DR1 increased during mucociliary differentiation during this period and became more similar to HNE cells obtained fresh by brush biopsy from nasal turbinates. Gene transcripts for B7-H3 and B7-H2 were abundantly expressed in cells cultured at the ALI, but neither their activities nor that of B7-2 was significantly altered during differentiation. IFN-gamma and TNF-alpha upregulated mRNA encoding for both HLA molecules, but not for the B7 molecules. This study describes, for the first time, the expression of B7-H3 and B7-H2 by HNE cells and thus expands the range of potential costimulatory signals through which these cells may interact with activated mucosal T lymphocytes. In addition, the results suggest that the extent of mucociliary differentiation of cultured cells may influence this capability

B7-H3 expression in donor T cells and host cells negatively regulates acute graft-versus-host disease lethality

“…1 A wide range of cells express B7-H3, including activated T cells, natural killer cells, dendritic cells (DCs), and macrophages [1][2][3] along with nonhematopoietic cells, including fibroblasts, synoviocytes, osteoblasts, and epithelial cells. [4][5][6] Although TLT-2 was identified as a receptor for B7-H3, 7 others have shown no evidence for this in mice or humans, 8 thus confounding elucidation of the biologic response of the B7-H3 pathway.…”

Regulation of Lysophosphatidic Acid-induced Epidermal Growth Factor Receptor Transactivation and Interleukin-8 Secretion in Human Bronchial Epithelial Cells by Protein Kinase Cδ, Lyn Kinase, and Matrix Metalloproteinases

“…Cell Culture-Primary human bronchial epithelial cells were isolated from normal human lung obtained from lung transplant donors following previously described procedures (41,42). The isolated P 0 HBEpCs were seeded at a density of 1.5 ϫ 10 4 cells/cm 2 onto vitrogen-coated (1:75 in sterile water, Cohesion, Palo Alto, CA) P-100 dishes in basal essential growth medium (supplied by Clonetics, BioWhittaker, Walkersville, MD) that was serum-free and supplemented with growth factors.…”

Differential regulation of hyaluronan-induced IL-8 and IP-10 in airway epithelial cells

“…Bronchial airways (2-to 18-mm diameter) from these sources were dissected free of connective tissue and parenchyma. Epithelial cells were prepared as previously described in detail (37) by digesting the tissues overnight at 4°C in 0.1% protease in Ham's F-12 medium containing antibiotics. FCS (10%) was added to neutralize the protease, and the epithelial cells were freed from the tissue by agitation and isolated by centrifugation.…”

“…When confluent, the cells were frozen at Ϫ70°C or immediately reseeded on collagencoated Falcon filter inserts or plastic dishes and grown to confluence under bronchial epithelial growth medium. When confluent, cells grown on inserts were transferred to the lower chamber to establish the cells at the air-liquid interface (37).…”