Expression of genes for Kisspeptin (
 KISS1
 ), Neurokinin B (
 TAC3
 ), Prodynorphin (
 PDYN
 ), and gonadotropin inhibitory hormone (
 RFRP
 ) across natural puberty in ewes

Li, Qun; Smith, Jeremy Troy; Henry, Belinda A.; Rao, Arathi; Pereira, Alda; Clarke, Iain J.

doi:10.14814/phy2.14399

Cited by 11 publications

(7 citation statements)

References 104 publications

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“…It may be that the increase in integrated density of mRNA for dynorphin within the ARC may reflect some type of intracellular feedback loop where decreased levels of peptide may result in increased mRNA expression. An increase in mRNA for dynorphin is consistent with the previous work of Li et al [65], wherein dynorphin mRNA expression was higher in postpubertal than prepubertal ewes. However, this result should be viewed with caution as that study compared prepubertal ovary-intact ewes to postpubertal ewes in the luteal phase of the estrous cycle and progesterone has been shown to increase dynorphin in female sheep [67,68].…”

Section: Discussionsupporting

confidence: 92%

“…Increased NKB synthesis would be consistent with the numerical increase in integrated density of expression per cell for NKB mRNA from five to eight to ten months of age, but these increases were not statistically significant. A lack of significant change in NKB mRNA expression is consistent with a previous study in sheep that reported no change in total cell numbers for NKB or the amount of mRNA for NKB per cell amongst ovary-intact prepubertal female sheep, postpubertal female sheep in the luteal phase of the estrous cycle, and postpubertal sheep that had been ovariectomized for one week [65].…”

Section: Discussionsupporting

confidence: 90%

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Kisspeptin, Neurokinin B, and Dynorphin Expression during Pubertal Development in Female Sheep

Aerts

Harlow

Griesgraber

et al. 2021

Biology

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The neural mechanisms underlying increases in gonadotropin-releasing hormone (GnRH) and luteinizing hormone (LH) secretion that drive puberty onset are unknown. Neurons coexpressing kisspeptin, neurokinin B (NKB), and dynorphin, i.e., KNDy neurons, are important as kisspeptin and NKB are stimulatory, and dynorphin inhibitory, to GnRH secretion. Given this, we hypothesized that kisspeptin and NKB expression would increase, but that dynorphin expression would decrease, with puberty. We collected blood and hypothalamic tissue from ovariectomized lambs implanted with estradiol at five, six, seven, eight (puberty), and ten months of age. Mean LH values and LH pulse frequency were the lowest at five to seven months, intermediate at eight months, and highest at ten months. Kisspeptin and NKB immunopositive cell numbers did not change with age. Numbers of cells expressing mRNA for kisspeptin, NKB, or dynorphin were similar at five, eight, and ten months of age. Age did not affect mRNA expression per cell for kisspeptin or NKB, but dynorphin mRNA expression per cell was elevated at ten months versus five months. Thus, neither KNDy protein nor mRNA expression changed in a predictable manner during pubertal development. These data raise the possibility that KNDy neurons, while critical, may await other inputs for the initiation of puberty.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 90%

Kisspeptin, Neurokinin B, and Dynorphin Expression during Pubertal Development in Female Sheep

Aerts

Harlow

Griesgraber

et al. 2021

Biology

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“…Kisspeptin drives pulsatile GnRH secretion, and the response in terms of LH secretion (which reflects GnRH) increases as calves progress toward puberty (Nakada et al, 2002;Alamilla Rodríguez et al, 2017). The number of Kiss cells in the caudal region of the arcuate nucleus increases during puberty in sheep (Li et al, 2020), so the timing of this neuroendocrine event could be a factor in the difference in age at puberty in POS and NEG cows. Thus, if less Kiss is produced in NEG heifers than in POS heifers in the prepubertal period, this might affect endogenous GnRH and LH secretion.…”

Section: Discussionmentioning

confidence: 99%

Response to kisspeptin and gonadotropin-releasing hormone agonist administration in Holstein-Friesian dairy heifers with positive or negative genetic merit for fertility traits

Flay

Reed

Kuhn-Sherlock

et al. 2022

Journal of Dairy Science

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Previous research has identified that Holstein-Friesian dairy heifers with positive (POS) genetic merit for fertility traits (FertBV) reach puberty earlier than heifers with negative (NEG) FertBV. The hypothalamuspituitary-gonadal (HPG) axis is functional in heifers before the onset of puberty, with increased LH release evident as heifers progress toward puberty. We investigated the functionality of the HPG axis in peripubertal Holstein-Friesian dairy heifers with divergent POS or NEG FertBV, hypothesizing that the earlier puberty onset of POS heifers is associated with earlier activation of the HPG axis than in NEG heifers. In experiment 1, we tested the dose responsiveness of POS heifers to an intravenous injection of either kisspeptin [Kiss; 2, 4, or 8 µg/kg of body weight (BW); n = 3 per dose] or a GnRH agonist (buserelin; 5, 10, or 20 ng/kg of BW; n = 3 per dose). The use of these 2 agonists investigates the status of the HPG axis in both the hypothalamus (Kiss) and pituitary (buserelin) glands. Doses of 4 µg/ kg BW of Kiss and 10 ng/kg BW of buserelin produced submaximal LH responses and were used in experiment 2, in which previously unused POS (n = 22) and NEG (n = 18) FertBV heifers were challenged with both agonists at 10 and 12 mo of age in a partial crossover design. Heifers were randomly allocated to treatment groups, balanced for age and BW. The LH response to buserelin was greater in POS heifers than NEG heifers at 10 mo of age, with no difference in response at 12 mo. The FSH response to buserelin and the LH and FSH responses to Kiss did not differ between the POS and NEG heifers at either age. These results indicate an association between divergent genetic merit for fertility and the LH release to buserelin at 10 mo of age, supporting the hypothesis that gonadotropin respon-siveness to a GnRH agonist is more advanced in POS heifers than in NEG heifers.

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“…KISS1 expression in the arcuate nucleus increases during puberty in ewes and may be a causative factor in pubertal activation of the reproductive axis. Furthermore, the decrease in RFRP expression may be a factor in the initiation of puberty ( Li et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Sequencing-Based Mining of Genes Associated With Pubertal Initiation in Dolang Sheep

et al. 2022

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Improving the fertility of sheep is an important goal in sheep breeding as it greatly increases the productivity. Dolang sheep is a typical representative breed of lamb in Xinjiang and is the main local sheep breed and meat source in the region. To explore the genes associated with the initiation of puberty in Dolang sheep, the hypothalamic tissues of Dolang sheep prepubertal, pubertal, and postpubertal periods were collected for RNA-seq analysis on the Illumina platform, generating 64.08 Gb clean reads. A total of 575, 166, and 648 differentially expressed genes (DEGs) were detected in prepuberty_vs._puberty, postpuberty_vs._prepuberty, and postpuberty_vs._puberty analyses, respectively. Based on Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, the related genes involved in the initiation of puberty in Dolang sheep were mined. Ten genes that have direct or indirect functions in the initiation of puberty in Dolang sheep were screened using the GO and KEGG results. Additionally, quantitative real-time PCR was used to verify the reliability of the RNA-Seq data. This study provided a new approach for revealing the mechanism of puberty initiation in sheep and provided a theoretical basis and candidate genes for the breeding of early-pubertal sheep by molecular techniques, and at the same time, it is also beneficial for the protection, development, and utilization of the fine genetic resources of Xinjiang local sheep.

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Expression of genes for Kisspeptin ( KISS1 ), Neurokinin B ( TAC3 ), Prodynorphin ( PDYN ), and gonadotropin inhibitory hormone ( RFRP ) across natural puberty in ewes

Cited by 11 publications

References 104 publications

Kisspeptin, Neurokinin B, and Dynorphin Expression during Pubertal Development in Female Sheep

Kisspeptin, Neurokinin B, and Dynorphin Expression during Pubertal Development in Female Sheep

Response to kisspeptin and gonadotropin-releasing hormone agonist administration in Holstein-Friesian dairy heifers with positive or negative genetic merit for fertility traits

Transcriptome Sequencing-Based Mining of Genes Associated With Pubertal Initiation in Dolang Sheep

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