Expression of genes related to sweet taste receptors and monosaccharides transporters along the gastrointestinal tracts at different development stages in goats

Tao, Ran; Li, Hengzhi; Liu, Yong; Tang, Shaoxun; Han, Xuefeng; Wang, Min; He, Zhixiong; Kang, Jian; Yan, Qiongxian; Tan, Zhiliang; Zhou, Chuanshe

doi:10.1016/j.livsci.2016.04.010

Cited by 6 publications

(6 citation statements)

References 35 publications

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“…In addition, SGLT1 regulation may occur during translation or post-translation [ 72 ]. The SGLT1 expression and activity exhibits a diurnal rhythm [ 73 ], and glucose transporters are expressed at different magnitudes along the gastrointestinal tract [ 74 ], hence the difference in findings of the different studies.…”

Section: Discussionmentioning

confidence: 99%

Diet Preference, Feed Efficiency and Expression of the Sodium-Dependent Glucose Transporter Isoform 1 and Sweet Taste Receptors in the Jejunum of Lambs Supplemented with Different Flavours

Mwangi

Dallasheh

Kalyesubula

et al. 2023

Animals

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This study investigated the effect of dietary flavour supplements on the preference, feed efficiency and expression of the sweet taste receptor family 1 members 2 and 3 (T1R2 + T1R3), and sodium-glucose linked transporter 1 (SGLT1) genes in the lambs’ small intestines. Eight, five-month-old, Israeli crossbred Assaf lambs were offered 16 different non-nutritive commercial flavours in rolled barley and ground corn. Capsicum and sucram were the most preferred non-aroma flavours (p = 0.020), while milky (p < 0.001) was the most preferred powder-aroma flavour. For the metabolic and relative gene expression study, eight lambs were randomly assigned to either sucram, capsicum, a mix containing sucram and capsicum at 1:1 ratio or no flavour for control in a 4 × 2 cross-over design. The total collection of urine (females only), faeces and refusals was carried out, and T1R2, T1R3 and SGLT1 relative gene expression evaluated from the proximal jejunum biopsies. Flavour had no significant effect on the feed intake (p = 0.934), but capsicum increased the average daily weight gain per metabolic body weight (p = 0.049). The T1R3 gene was expressed highest in the mix treatment (1.7; p = 0.005). Collectively, our findings indicate that flavours can be used to motivate feed acceptance and improve the weight gain in lambs.

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Section: Discussionmentioning

confidence: 99%

Diet Preference, Feed Efficiency and Expression of the Sodium-Dependent Glucose Transporter Isoform 1 and Sweet Taste Receptors in the Jejunum of Lambs Supplemented with Different Flavours

Mwangi

Dallasheh

Kalyesubula

et al. 2023

Animals

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“…GLUTs are a family of proteins involved in carbohydrate transport across the cell membrane in an energy‐free manner. Additionally, dietary concentrate supplementation can contribute to the greater expression of GLUT2 and GLUT5 in the small intestines of goats (Jiao et al, 2019; Ran, Li, Liu, Tang, et al, 2016). These results were in line with those of the present study.…”

Section: Discussionmentioning

confidence: 99%

“…A parallel study indicated similar results regarding the expression of SGLT3 in the rumen epithelium under HG diets (Tian et al, 2020). The function of SGLT3, which is considered a carbohydrate sensing receptor rather than a carbohydrate transporter, remains controversial, and further studies should be implemented (Ran, Li, Liu, Tang, et al, 2016). Parallel studies indicated that the total weight gain of HG group was promoted, which may be attributed to the richer amount of carbohydrate of HG group.…”

Section: Discussionmentioning

confidence: 99%

The expression of nutrient chemosensing gate molecules in the ileum and colon is altered for goats fed on a high‐grain diet

Tian

Jiao

et al. 2022

Animal Science Journal

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Limited knowledge is clarified about alterations in the related expression of nutrient chemosensors in the distal small intestine and hindgut under a high-grain (HG) diet in small ruminants. Herein, this study was performed to investigate the expression changes related to nutrient sensing and transport in the ileal and colonic epithelium of goats in response to feeding an HG diet. Twelve Liuyang black goats (similar age and weight) were randomly assigned into two groups: an HG diet (concentrate: hay = 90:10) and a CON diet (concentrate: hay = 55:45). Immunohistochemistry was applied to detect morphological changes in the gut epithelium together with altered expression of chemosensors in the ileum and colon. The results showed that feeding an HG diet increased ileal villus height and depth and induced mucosal sloughing in the colon. The expressions of the nutrient transporters GLUT2, GLUT5, SGLT2, CD36, rBAT, EAAT3, and LAT2 and sensing receptors GPR43 and T1R1 were promoted in the ileum under HG conditions. Moreover, feeding an HG diet also enhanced the expression of GLUT2, SGLT2, CD36, and GPR43 in the colon. These findings indicate that adaptation of the gastrointestinal tract to the HG diet promoted the absorption of glucose, fatty acids, and amino acids in goats.

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“…Primers used for amplification target genes were either designed using online tools ( and ) based on related gene sequences of goats or extracted from reported references (shown in Table ). β-Actin and 18s-RNA were selected as housekeeping genes to normalize the expression of target genes. , All primers were synthesized by Sangon Biotech (Shanghai, China). The specificity of each of the designed primers was checked via online Primer-BLAST () and subsequent gel electrophoresis analysis, cloning, and sequencing of PCR products of designed primers, as well as melt curve analysis during quantitative real-time PCR.…”

Section: Methodsmentioning

confidence: 99%

“…β-Actin and 18s-RNA were selected as housekeeping genes to normalize the expression of target genes. 7,8 All primers were synthesized by Sangon Biotech (Shanghai, China). The specificity of each of the designed primers was checked via online Primer-BLAST (http://www.ncbi.nlm.nih.gov/tools/primer-blast/) and subsequent gel electrophoresis analysis, cloning, and sequencing of PCR products of designed primers, as well as melt curve analysis during quantitative real-time PCR.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Inferring the Skeletal Muscle Developmental Changes of Grazing and Barn-Fed Goats from Gene Expression Data

Huang

Jiao

Tan

et al. 2016

J. Agric. Food Chem.

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Thirty-six Xiangdong black goats were used to investigate age-related mRNA and protein expression levels of some genes related to skeletal muscle structural proteins, MRFs and MEF2 family, and skeletal muscle fiber type and composition during skeletal muscle growth under grazing (G) and barn-fed (BF) feeding systems. Goats were slaughtered at six time points selected to reflect developmental changes of skeletal muscle during nonrumination (days 0, 7, and 14), transition (day 42), and rumination phases (days 56 and 70). It was observed that the number of type IIx in the longissimus dorsi was increased quickly while numbers of type IIa and IIb decreased slightly, indicating that these genes were coordinated during the rapid growth and development stages of skeletal muscle. No gene expression was affected (P > 0.05) by feeding system except Myf5 and Myf6. Protein expressions of MYOZ3 and MEF2C were affected (P < 0.05) by age, whereas PGC-1α was linearly decreased in the G group, and only MYOZ3 protein was affected (P < 0.001) by feeding system. Moreover, it was found that PGC-1α and MEF2C proteins may interact with each other in promoting muscle growth. The current results indicate that (1) skeletal muscle growth during days 0-70 after birth is mainly myofiber hypertrophy and differentiation, (2) weaning affects the expression of relevant genes of skeletal muscle structural proteins, skeletal muscle growth, and skeletal muscle fiber type and composition, and (3) nutrition or feeding regimen mainly influences the expression of skeletal muscle growth genes.

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Expression of genes related to sweet taste receptors and monosaccharides transporters along the gastrointestinal tracts at different development stages in goats

Cited by 6 publications

References 35 publications

Diet Preference, Feed Efficiency and Expression of the Sodium-Dependent Glucose Transporter Isoform 1 and Sweet Taste Receptors in the Jejunum of Lambs Supplemented with Different Flavours

Diet Preference, Feed Efficiency and Expression of the Sodium-Dependent Glucose Transporter Isoform 1 and Sweet Taste Receptors in the Jejunum of Lambs Supplemented with Different Flavours

The expression of nutrient chemosensing gate molecules in the ileum and colon is altered for goats fed on a high‐grain diet

Inferring the Skeletal Muscle Developmental Changes of Grazing and Barn-Fed Goats from Gene Expression Data

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