Expression of genes responsible for ethylene production and wilting are differently regulated in carnation (Dianthus caryophyllus L.) petals

Kosugi, Yusuke; Shibuya, Kazuhiko; Tsuruno, Nanako; Iwazaki, Yujiro; Yoshioka, T.; Hashiba, Teruyoshi; Satoh, Shigeru

doi:10.1016/s0168-9452(00)00314-9

Cited by 31 publications

(21 citation statements)

References 28 publications

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“…Close relationships between the transcription levels of ACC synthase genes and ACC synthase activity and ethylene production rates have been reported in carnation petal senescence (Iwazaki et al, 2004;Kosugi et al, 2000Kosugi et al, , 2002ten Have and Woltering, 1997;Woodson et al, 1992). It is assumed, therefore, that undetectable levels of DcACS1 transcripts in the petals of 'WC' could contribute to the absence of ethylene production in this cultivar.…”

Section: Role Of the Gynocium In Natural Senescence Of Carnation Flowerssupporting

confidence: 61%

“…It has been believed that ethylene production and wilting are regulated in concert and cannot be separated, since the two events occur in close parallel in senescing carnation petals. Kosugi et al (2000) challenged this problem by using an ACC oxidase-suppressed transgenic carnation line, sACO-1, harboring an sACO transgene, i.e., DcACO1 cDNA in sense orientation under the control of a strong constitutive promoter, CaMV35S promoter, with additional enhancer sequences (Mitsuhara et al, 1996). Cut flowers of the sACO-1 line produced only a trace amount of ethylene during the senescence period, and had a vase life about 2-fold longer than the flowers of the parent 'Nora' carnation.…”

Section: Factors Responsible For Inducing Ethylenementioning

confidence: 99%

“…As described above, Kosugi et al (2000Kosugi et al ( , 2002) generated a transgenic carnation line (sACO-1) transformed with DcACO1 cDNA in sense orientation (sACO transgene) using 'Nora' carnation. The transfor- mation vector was constructed using pMLH2113-GUS, and the transgene was driven by CaMV35S promoter (Mitsuhara et al, 1996).…”

Section: Generation Of Transgenic Carnation Deficient In Ethylene Promentioning

confidence: 99%

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Ethylene Production and Petal Wilting during Senescence of Cut Carnation (Dianthus caryophyllus) Flowers and Prolonging Their Vase Life by Genetic Transformation

Satoh

2011

J. Japan. Soc. Hort. Sci.

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Senescence of carnation flowers is characterized by autocatalytic ethylene production from petals and subsequent wilting of the petals. Recent studies on the regulation of ethylene production and wilting in senescing carnation petals revealed that (1) petal senescence is triggered by ethylene evolved from the gynoecium during natural senescence, (2) ethylene production in the gynoecium is induced by a factor(s) other than pollination signals in carnation flowers lacking anthers, (3) there are two subsets of ethylene responses in the petals, one responsible for autocatalytic ethylene production and the other for wilting, (4) expression of genes involved in the execution of petal withering is differently regulated between ethylene-dependent or -independent senescence. Furthermore, it was revealed that the generation of transgenic carnation without detectable ethylene production is useful to prolong the vase life of cut carnation flowers.

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Section: Role Of the Gynocium In Natural Senescence Of Carnation Flowerssupporting

confidence: 61%

Section: Factors Responsible For Inducing Ethylenementioning

confidence: 99%

Section: Generation Of Transgenic Carnation Deficient In Ethylene Promentioning

confidence: 99%

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Ethylene Production and Petal Wilting during Senescence of Cut Carnation (Dianthus caryophyllus) Flowers and Prolonging Their Vase Life by Genetic Transformation

Satoh

2011

J. Japan. Soc. Hort. Sci.

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“…For the construction of an expression plasmid for carnation DcACO1, the entire coding region with NdeI and BamHI recognition sites at the 5′-and the 3′ ends, respectively, was amplified from DcACO1 cDNA (Kosugi et al, 2000) by PCR with the upstream and downstream primers, 5′-CCCCCATATGGCAAACATTGTC AACTTCCC-3′ and 5′-CCCCGGATCCTCAAGCAGTT GGAATGGGAC-3′, respectively. The amplified product was digested with NdeI and BamHI.…”

Section: Preparation Of Acc Oxidase By Expression Of Carnation Cdna Imentioning

confidence: 99%

2,4-Pyridinedicarboxylic Acid Prolongs the Vase Life of Cut Flowers of Spray Carnations

Satoh

Kosugi

Sugiyama

et al. 2014

J. Japan. Soc. Hort. Sci.

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2,4-Pyridinedicarboxylic acid (PDCA) is a structural analog of 2-oxoglutarate and has been shown to inhibit 2-oxoglutarate-dependent dioxygenases by competing with 2-oxoglutarate, and ethylene production in detached carnation flowers by competing with ascorbate on 1-aminocyclopropane-1-carboxylate (ACC) oxidase action. In the present study, the inhibition of ACC oxidase action by PDCA was confirmed with a recombinant enzyme produced in Escherichia coli from carnation DcACO1 cDNA. PDCA had various effects on ethylene production in cut 'Light Pink Barbara (LPB)' carnation flowers; ethylene production was accelerated or delayed in some flowers, whereas it did not change in others as compared to untreated control flowers. This varied action of PDCA may be caused by its possible combined actions; that is, inhibition of ACC oxidase action as well as its action on unidentified biochemical processes which use 2-oxoglutarate as a co-substrate, such as the biosynthesis and inactivation of gibberellins. Meanwhile, PDCA treatment significantly prolonged the vase life of bunches of cut 'LPB' carnation flowers; the magnitude of the extension of vase life was 53, 111, and 135% at 0.3, 1, and 2 mM PDCA, respectively, as compared with the non-treated control. Also, PDCA lengthened the vase life of 'Mule' carnation flowers. The present findings suggest the potential of PDCA as a preservative for cut flowers of spray carnations.

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“…5). The DcCP1, DcGST1, and DcbGal which lead to cell death during senescence of petals, were previously thought to be related to the induction of inrolling (Hong et al, 2000;Jones et al, 1995;Kosugi et al, 2000). However, recent research has shown that they are upregulated during senescence regardless of whether inrolling is observed (Otsu et al, 2007;Tanase et al, 2013).…”

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confidence: 99%

Regulation of Ethylene- and Senescence-related Genes in Pot Carnation Flowers during Flower Senescence

Tanase

Onozaki

2016

Hortic J

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We investigated differences in flower longevity, ethylene production, and ethylene sensitivity among pot carnation cultivars by quantitative PCR analysis. The flower life of 'Polaris' was significantly longer than that of 'Ariel' (control cultivar), 'Orange Duo', and 'Lemon Soft'. The flowers of 'Polaris' produced little ethylene, and had low expression of the ethylene biosynthesis genes DcACS1 and DcACO1. The transcript levels of senescence-related (SR) genes DcCP1, DcbGal, and DcGST1 increased in petals of 'Ariel', 'Orange Duo', and 'Lemon Soft' at senescence, but were very low in 'Polaris'. These results suggest that the low ethylene production in 'Polaris' is caused by low expression of DcACS1 and DcACO1, as in long-life flowers of the cut carnation cultivars 'Miracle Rouge' and 'Miracle Symphony'. The ethylene sensitivity of 'Orange Duo' and 'Lemon Soft' was lower than that of both 'Ariel' and 'Polaris'. Petals of 'Orange Duo' and 'Lemon Soft' wilted, and inrolled more slowly than those of 'Ariel' and 'Polaris', despite the upregulation of ethylene biosynthesis genes (DcACS1 and DcACO1), DcCP1, DcbGal, DcGST1, and DcEIL3 in petals of all cultivars upon application of exogenous ethylene. These results imply that only the components related to the inrolling of petals are altered in these ethylene-insensitive cultivars.

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Expression of genes responsible for ethylene production and wilting are differently regulated in carnation (Dianthus caryophyllus L.) petals

Cited by 31 publications

References 28 publications

Ethylene Production and Petal Wilting during Senescence of Cut Carnation (Dianthus caryophyllus) Flowers and Prolonging Their Vase Life by Genetic Transformation

Ethylene Production and Petal Wilting during Senescence of Cut Carnation (Dianthus caryophyllus) Flowers and Prolonging Their Vase Life by Genetic Transformation

2,4-Pyridinedicarboxylic Acid Prolongs the Vase Life of Cut Flowers of Spray Carnations

Regulation of Ethylene- and Senescence-related Genes in Pot Carnation Flowers during Flower Senescence

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