Expression of Glucose-6-Phosphate Dehydrogenase and 6-Phosphogluconate Dehydrogenase in Oxidative Stress Induced by Long-Term Iron Toxicity in Rat Liver

Abstract

Reactive oxygen species (ROS) are highly reactive and oxygen-containing molecules that are derived by metabolic activities or from environmental sources. Toxicity of heavy metals including iron has the ability to generate ROS in all living organisms. The pentose phosphate pathway enzymes, which are glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase, produce nicotinamide adenine dinucleotide phosphate (NADPH) that enables cells to counterbalance the oxidative stress via the action of the glutathione system. The results presented here have shown that toxic and nontoxic levels of iron have a strong effect on the expression of both genes. While toxic levels of iron exhibited significant changes in enzyme activity, nontoxic levels had no effect on enzymes in rat liver. Our results are the first evidence to elucidate how oxidative stress induced by long-term iron toxicity affects both enzymes at the enzymatic and molecular level and also to determine any possible correlation between the enzymatic and molecular levels.

Differential proteomic analysis of synovial fluid from hip arthroplasty patients with a pseudotumor vs. Periprosthetic osteolysis

“…In both patient groups, results are consistent with the presence of oxidative stress, a characteristic response to metal products (particularly metal ions) released from metal wear and corrosion . Nevertheless, the absolute specificity and sensitivity for the pseudotumor group of 6‐phosphogluconate dehydrogenase, an enzyme that plays a major protective role against oxidative stress, suggest the presence of greater oxidative stress, which, as proposed by Scharf et al, might explain necrosis and the formation of pseudotumors. 6‐Phosphogluconate dehydrogenase, a rate‐limiting enzyme of the pentose phosphate pathway, catalyzes the reduction of NADP to NADPH and is therefore critical in counterbalancing oxidative stress via the glutathione system .…”

“…Nevertheless, the absolute specificity and sensitivity for the pseudotumor group of 6‐phosphogluconate dehydrogenase, an enzyme that plays a major protective role against oxidative stress, suggest the presence of greater oxidative stress, which, as proposed by Scharf et al, might explain necrosis and the formation of pseudotumors. 6‐Phosphogluconate dehydrogenase, a rate‐limiting enzyme of the pentose phosphate pathway, catalyzes the reduction of NADP to NADPH and is therefore critical in counterbalancing oxidative stress via the glutathione system . Toxic levels of iron, which increase oxidative stress, were recently shown to upregulate the expression of this enzyme .…”

Upregulation of Oxidative Stress Related Genes in a Chronic Kidney Disease Attributed to Specific Geographical Locations of Sri Lanka

“…The study by Jayasekara et al found 20% of the CKDu cases to have some severity of G6PD deficiency whereas only 2% of the healthy controls had the deficiency [13]. This enzyme is the first rate-limiting enzyme in the pentose phosphate pathway and is responsible for the production of NADPH which is needed in the glutathione reduction process [28]. However, the differential expressions seen in our study groups were not statistically significant on the one-way ANOVA.…”

Overexpression, purification and enzymatic characterization of a recombinant Arabian camel Camelus dromedarius glucose-6-phosphate dehydrogenase

“…Furthermore, several studies described significant roles of G6PD during abiotic and biotic stress in higher plants [10][11][12]. At the molecular level, G6PD has been well studied in many eutherian species including human, mouse, rats and plants [4,[12][13][14][15][16]. The domesticated one-humped Arabian camel, C. dromedarius, is one of the most important animals in the Arabian Peninsula.…”