Expression of glutathione-S-transferases isoenzymes and p53 in exfoliated human bladder cancer cells

Oğuztüzün, Serpil; Sezgin, Yasemin; Yazıcı, Sertaç; Firat, Pınar; Özhavzali, Müzeyyen; Özen, Haluk

doi:10.1016/j.urolonc.2009.08.001

Cited by 14 publications

(5 citation statements)

References 24 publications

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“…For example, several studies have explored the role of GST enzyme expression in urologic oncology, especially GSTP and GSTT, but most these studies have focused on bladder cancer. Although GST isoenzymes are not an effective marker of malignancy in urinary cytology, they can be used to construct the molecular biologic database for use in further research (Oğuztüzün et al, 2011). This study compared GSTT1 and GSTP1 expressions in UUT-UC tissue and normal appearing urothelium.…”

Section: Introductionmentioning

confidence: 99%

Glutathione S-Transferase Expression in Upper Urinary Tract Urothelial Carcinomas: a Taiwan Study

Chen

Wu²,

Tu³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Objectives: Glutathione S-transferase (GST) isoenzymes play important roles in resistance to cell apoptosis and carcinogenesis. We aimed to establish the relationship between GST expression and the prognosis of upper urinary tract urothelial carcinoma (UTT-UC) in Taiwan. Methods: This study retrospectively reviewed 46 patients with pathologically confirmed UUT-UC at Kaohsiung Medical University Hospital. In each patient, expression of GSTT1 and GSTP1 was compared between urothelial carcinoma and normal urothelial cells by Western blotting. Results: GSTP1 expression in the UUT-UC cells was significantly higher than that in normal urothelial cells (1.6 fold, p<0.001). Expression of GSTT1 was significantly associated with the invasiveness of the carcinoma (p=0.006). Conclusions: In UUT-UC, GSTP1 might be a potential tumor marker, whereas high GSTT1 expression could be used as an indicator of cancer progression. This study is the first to demonstrate potential applications of different GST isoenzymes for biomolecular analysis of UUT-UCs in Taiwan.

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Section: Introductionmentioning

confidence: 99%

Glutathione S-Transferase Expression in Upper Urinary Tract Urothelial Carcinomas: a Taiwan Study

Chen

Wu²,

Tu³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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“…Glutathione transferase is an important detoxification enzyme and plays the main role in xenobiotics transformation, especially PAHs. Isozyme GST π is particularly involved in carcinogenesis process [40–43]. Our studies showed a significantly higher GST π urine concentration in BC patients than in the healthy group.…”

Section: Discussionmentioning

confidence: 53%

Preliminary Evaluation of the Diagnostic Usefulness of Uroplakin 2 with an Assessment of the Antioxidant Potential of Patients with Bladder Cancer

Matuszewski

Szymańska

Długosz

et al. 2018

BioMed Research International

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Background Urothelial carcinoma is the most common type of bladder cancer (BC). It makes up more than 90% of all bladder cancers. Uroplakins are tissue-specific, glycoproteins, playing a role in the construction and function of urothelium. The emergence of uroplakins in the urine and/or plasma may be of potential importance in the early detection of BC. In our study, the diagnostic value of plasma and urine uroplakin 2 (UP2) concentration in bladder cancer was investigated, with an assessment of the antioxidant potential of BC patients. The correlation between UP2, total antioxidant capacity (TAC), and concentration of glutathione (GSH) was also examined. Materials and Methods This study included 61 BC patients and 33 healthy controls. UP2 concentration was estimated by the immunoenzymatic method (ELISA). TAC and GSH were determined in spectrophotometrically methods. Results UP2 concentration in BC patients was significantly higher (p≤0.001) both in plasma and in urine compared to the control groups (C). TAC concentration in urine (p≤0.001) and GSH concentration in plasma (p=0.047) were significantly lower in BC group compared to the C group. The high specificity and sensitivity for UPK2 in plasma (76%, 80%, respectively) and urine (88%, 84%, respectively) were observed. Positive correlations were observed between concentration of UP2 in plasma and TAC concentration in urine and between UP2 concentration in plasma and GSH concentration in the same material. Conclusion The study showed the early diagnostic value of urine and plasma UP2 in BC. There was a decrease in UP2 concentration in the urine of patients with the development of BC. The decrease of antioxidant systems (TAC, GSH) indicates their relationship with the BC process. Based on the obtained results, it is justified to continue the study in a larger group of patients with BC.

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“…[ 22 – 24 ] Though an article reported that there was no significant difference in staining intensity for GSTM4 between benign and normal epithelium cells in BCa. [ 25 ] Whether GSTM4 plays a critical role in BCa is not certain, considering the limitations of that study. For example, it only investigated the expression of related genes in exfoliated cells and only conducted immunocytochemical methods to show the expression of the genes.…”

Section: Discussionmentioning

confidence: 97%

Shared genetic links between bladder cancer and obesity-related traits: A conjunctional false discovery rate study

Chen,

Li,

et al. 2023

Medicine

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Bladder cancer (BCa) is a common cancer worldwide and is often linked with obesity-related comorbidities, but little is known about the underlying genetic mechanisms. To investigate these mechanisms, we used various quantitative tools, including conditional quantile-quantile (Q-Q) plots, conditional false discovery rate (cFDR), and conjunctional conditional false discovery rate (ccFDR), to explore the pleiotropic enrichment of risk loci between BCa and obesity-related traits. We also performed an expression quantitative trait locus (eQTL) analysis to assess the relationship between shared risk loci and gene expression. Finally, we conducted functional annotation using Kyoto Encyclopedia of Genes and Genomes (KEGG) and gene ontology (GO) analysis. Our findings indicated that there was successive enrichment for a range of obesity-related traits, including body fat percentage, body mass index, fasting insulin, type 2 diabetes mellitus, fasting glucose, high-density lipoprotein cholesterol, total triglycerides, and waist-to-hip ratio. Using the tools mentioned above, we identified 18 significant SNPs and 18 closely related genes (cFDR<0.01) under the condition of 8 obesity-related traits. The SNPs included rs143004880, rs73301337, rs10798572, rs11594929, rs17019138, rs2877, rs149795948, rs142509736, rs12727575, rs1571277, rs12131828, rs635634, rs76895963, rs118081211, rs7044247, rs138895564, rs4135275, and rs148023060. Additionally, we identified 15 novel loci using ccFDR, including rs143004880, rs73301337, rs10798572, rs11594929, rs17019138, rs2877, rs142509736, rs1571277, rs635634, rs76895963, rs12131828, rs118081211, rs7044247, rs138895564, and rs4135275. Of the 2 significant loci that modify gene expression, rs12131828 and rs635634 were identified. The functional annotation indicated that the conditional risk genes mainly participated in the regulation of gene silencing. Our study provided evidence of pleiotropic enrichment between BCa and 8 obesity-related traits, and we identified potential genetic mechanisms underlying this relationship. These findings may help in developing targeted clinical treatments for BCa.

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Expression of glutathione-S-transferases isoenzymes and p53 in exfoliated human bladder cancer cells

Cited by 14 publications

References 24 publications

Glutathione S-Transferase Expression in Upper Urinary Tract Urothelial Carcinomas: a Taiwan Study

Glutathione S-Transferase Expression in Upper Urinary Tract Urothelial Carcinomas: a Taiwan Study

Preliminary Evaluation of the Diagnostic Usefulness of Uroplakin 2 with an Assessment of the Antioxidant Potential of Patients with Bladder Cancer

Shared genetic links between bladder cancer and obesity-related traits: A conjunctional false discovery rate study

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