Expression of gonadotropin-releasing hormone receptor in cerebral cortical neurons of embryos and adult rats

Quintanar, J. Luis; Salinas, Eva; González, Rodolfo

doi:10.1016/j.neulet.2006.06.077

Cited by 19 publications

(12 citation statements)

References 18 publications

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“…GnRH receptors and some isolated GnRH neurons were also observed in cerebral cortex in humans and rats (31,76,97). Interestingly, increases of GnRH levels were detected in the cortex when migration of GnRH neurons toward their natural location was impaired, as in mice overexpressing GAD-67 in GnRH neurons (43) or in netrin 1 knockout mice (81).…”

Section: Gnrh Content and Mrna Expressionmentioning

confidence: 98%

Lack of functional GABA_B receptors alters GnRH physiology and sexual dimorphic expression of GnRH and GAD-67 in the brain

Catalano

Giorgio

Bonaventura

et al. 2010

American Journal of Physiology-Endocrinology and Metabolism

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GABA, the main inhibitory neurotransmitter, acts through GABAA/C and GABAB receptors (GABABRs); it is critical for gonadotropin regulation. We studied whether the lack of functional GABABRs in GABAB1 knockout (GABAB1KO) mice affected the gonadotropin axis physiology. Adult male and female GABAB1KO and wild-type (WT) mice were killed to collect blood and tissue samples. Gonadotropin-releasing hormone (GnRH) content in whole hypothalami (HT), olfactory bulbs (OB), and frontoparietal cortexes (CT) were determined (RIA). GnRH expression by quantitative real-time PCR (qRT-PCR) was evaluated in preoptic area-anterior hypothalamus (POA-AH), medial basal-posterior hypothalamus (MBH-PH), OB, and CT. Pulsatile GnRH secretion from hypothalamic explants was measured by RIA. GABA, glutamate, and taurine contents in HT and CT were determined by HPLC. Glutamic acid decarboxylase-67 (GAD-67) mRNA was measured by qRT-PCR in POA-AH, MBH-PH, and CT. Gonadotropin content, serum levels, and secretion from adenohypophyseal cell cultures (ACC) were measured by RIA. GnRH mRNA expression was increased in POA-AH of WT males compared with females; this pattern of expression was inversed in GABAB1KO mice. MBH-PH, OB, and CT did not follow this pattern. In GABAB1KO females, GnRH pulse frequency was increased and GABA and glutamate contents were augmented. POA-AH GAD-67 mRNA showed the same expression pattern as GnRH mRNA in this area. Gonadotropin pituitary contents and serum levels showed no differences between genotypes. Increased basal LH secretion and decreased GnRH-stimulated gonadotropin response were observed in GABAB1KO female ACCs. These results support the hypothesis that the absence of functional GABABRs alters GnRH physiology and critically affects sexual dimorphic expression of GnRH and GAD-67 in POA-AH.

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Section: Gnrh Content and Mrna Expressionmentioning

confidence: 98%

Lack of functional GABA_B receptors alters GnRH physiology and sexual dimorphic expression of GnRH and GAD-67 in the brain

Catalano

Giorgio

Bonaventura

et al. 2010

American Journal of Physiology-Endocrinology and Metabolism

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“…It was reported that GnRH and GnRH-R are also produced in a variety of tissues outside the pituitary [2,3,7,20]. However, little is known about the molecular mechanisms and physiological settings in which GnRH exerts its activities on extrapituitary cells.…”

Section: Discussionmentioning

confidence: 99%

“…The GnRH expression has been demonstrated in the olfactory placode of 12.5-days-old rat embryo [6]. We have recently reported the expression of GnRH-R in cerebral cortical neurons of 14-days-old embryos and adult rats, and that incubation with GnRH modified GnRH-R mRNA expression [7]. At the present, little information is available on the function and regulation of extrapituitary GnRH.…”

Section: Introductionmentioning

confidence: 99%

Neurotrophic Effects of GnRH on Neurite Outgrowth and Neurofilament Protein Expression in Cultured Cerebral Cortical Neurons of Rat Embryos

Quintanar

Salinas

2007

Neurochem Res

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The presence of GnRH receptor in cerebral cortical neurons of rat embryos and adult rats has been described. In this work, we studied the effects of GnRH on outgrowth and length of neurites and cytoskeletal neurofilament proteins expression (NF-68 and NF-200 kDa) by immunoblot of cultured cerebral cortical neurons of rat embryos. Our results show that GnRH increases both outgrowth and length of neurites accompanied by an increase in neurofilaments expression. It is conceivable that GnRH plays a role in neuronal plasticity parallel to its gonadal function.

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“…Immunohistochemical method: To demonstrate immunoreactivity of the LH, FSH, TSH and PRL hormones, the avidin-biotinperoxidase complex method was practiced (Hsu et al 1981), using the kit (Vectastin ABC kit, Dimension Laboratories inc., CA, U.S.A.) with slight modifications (Quintanar et al 2007b). Primary antibodies LH (AntiRat LH serum for RiA), PRL (Anti-Rat PRL serum for RiA), FSH (Anti-Rat FSH serum for RiA) and TSH (Anti-Rat TSH serum for RiA) were used at 1:20, 1:30, 1:50 and 1:100 dilutions, respectively; which are intermediate values according to manufacturer's suggestion and previous results (Salinas et al 2004).…”

Section: Rotifer Collection and Culturementioning

confidence: 99%

Immunodetection of Luteinizing Hormone (LH), Follicle-Stimulating Hormone (FSH), Thyroid Stimulating Hormone (TSH) and Prolactin (PRL) in Brachionus calyciflorus (Rotifera: Monogononta)

Alvarado-Flores¹,

Montoya-Garcia²,

Ventura-Juárez³

et al. 2008

RBT

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Abstract:The endocrine system controls and coordinates behavioral, biochemical, and physiological processes through signal mechanisms using neuropeptides or products of neurosecretory cells. Among invertebrates, this system is poorly studied in rotifers, in which estrogens and androgens significantly affect sexual reproduction. This is the first report of the presence of the Luteinizing Hormone (LH), Follicle-Stimulating Hormone (FSH), Thyroid Stimulating Hormone (TSH) and Prolactin (PRL) in rotifers. Analyses included the avidin-biotin-peroxidase complex method with primary antibodies LH (Anti-Rat LH serum for RiA), PRL (Anti-Rat PRL serum for RiA), FSH (Anti-Rat FSH serum for RiA) and TSH (Anti-Rat TSH serum for RiA). These hormones were found in females, males and parthenogenetic and sexual eggs of the freshwater Brachionus calyciflorus. The immunoreactivity of FSH, LH, TSH and PRL in females was observed in: ovaries, cerebrum, mastax, stomach, lorica, and the stomach gland. However, in males LH was observed only at the trochal disk and cerebrum. The hormones FSH, TSH and PRL, were observed in testicles, contractil vesicles, and cementary gland of males. Regarding amictic or parthenogenetic eggs, the hormones LH, FSH, TSH, and PRL were located mainly in the micromeres, and the staining in the macromeres was weak. On the other hand, in the mictic or sexual eggs the inner shell is stained for the hormones PRL and LH, opposite to the staining of FSH and TSH, located mainly in the embryo. in general, immuno-reactivity was observed in areas important for the reproductive, excretory, digestive and developmental processes. Rev. Biol. Trop. 57 (4):

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Expression of gonadotropin-releasing hormone receptor in cerebral cortical neurons of embryos and adult rats

Cited by 19 publications

References 18 publications

Lack of functional GABA_B receptors alters GnRH physiology and sexual dimorphic expression of GnRH and GAD-67 in the brain

Lack of functional GABA_B receptors alters GnRH physiology and sexual dimorphic expression of GnRH and GAD-67 in the brain

Neurotrophic Effects of GnRH on Neurite Outgrowth and Neurofilament Protein Expression in Cultured Cerebral Cortical Neurons of Rat Embryos

Immunodetection of Luteinizing Hormone (LH), Follicle-Stimulating Hormone (FSH), Thyroid Stimulating Hormone (TSH) and Prolactin (PRL) in Brachionus calyciflorus (Rotifera: Monogononta)

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Expression of gonadotropin-releasing hormone receptor in cerebral cortical neurons of embryos and adult rats

Cited by 19 publications

References 18 publications

Lack of functional GABAB receptors alters GnRH physiology and sexual dimorphic expression of GnRH and GAD-67 in the brain

Lack of functional GABAB receptors alters GnRH physiology and sexual dimorphic expression of GnRH and GAD-67 in the brain

Neurotrophic Effects of GnRH on Neurite Outgrowth and Neurofilament Protein Expression in Cultured Cerebral Cortical Neurons of Rat Embryos

Immunodetection of Luteinizing Hormone (LH), Follicle-Stimulating Hormone (FSH), Thyroid Stimulating Hormone (TSH) and Prolactin (PRL) in Brachionus calyciflorus (Rotifera: Monogononta)

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Lack of functional GABA_B receptors alters GnRH physiology and sexual dimorphic expression of GnRH and GAD-67 in the brain

Lack of functional GABA_B receptors alters GnRH physiology and sexual dimorphic expression of GnRH and GAD-67 in the brain