Expression of growth and differentiation Factor 9 and cognate receptors during final follicular growth in cattle

Haas, Cristina; Rovani, Monique Tomazele; Oliveira, Fernando Caetano de; Vieira, Arnaldo Diniz; Bordignon, Vilceu; Gonçalves, Paulo Bayard Dias; Ferreira, Rogério; Gasperin, Bernardo Garziera

doi:10.21451/1984-3143-ar789

Cited by 7 publications

(8 citation statements)

References 21 publications

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“…When comparing rodents with an incomplete estrous cycle (mice, rats) to animals with a complete estrous cycle (humans, goats, pigs and cows), GDF9 and BMP15 appear in different tissues. The mRNA expression of the transforming growth factors-beta studied was detected exclusively in oocytes of rodents (13), while in other species, including humans ( 14), goats (15), sheep (8,16), pigs (17) and cows (18,19), BMP15 and GDF9 were expressed in cumulus cells as well as in oocytes, in agreement with the result obtained in this work. Additionally, in the present study, BMP15 and GDF9 were also expressed in the pellet of cells, where granulosa and theca cells were expresed.…”

Section: Discussionsupporting

confidence: 89%

“…GDF9 and BMP15 growth factors bind to specific receptors to trigger its mechanisms of action on the target cell. TGFβ-R1 mRNA expression was detected in granulosa cells of sheep and cows (19,22). BMPR-IB mRNA expression has been observed in goat, sheep, pig and bovine follicles (8,23,24), nevertheless here we report for the first time that TGFβ-R1 and BMPR-IB mRNA are expressed in bovine oocytes.…”

Section: Discussionmentioning

confidence: 99%

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Expresión génica del factor de crecimiento BMP15, GDF9, FGF2 y sus receptores en células foliculares bovinas

Reineri¹,

Coria²,

Barrionuevo³

et al. 2018

Rev MVZ Córdoba

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Introduction. Growth and follicular maturation involve transformations of various components of the follicle, such as the oocyte, granulosa and techa cells. Several growth factors, including differentiation growth factor 9 (GDF9), bone morphogenic protein 15 (BMP15) and basic fibroblast growth factor (FGF2) are important for follicular development and oocyte maturation, by its ability to increase the proliferation of granulosa, techa cells and the ovarian stroma. Objetive. Evaluate mRNA expression of GDF9, BMP15, FGF2 and their main receptors, transforming growth factor beta receptor 1 (TGFβ-R1), bone morphogenetic protein receptor, type IB (BMPR-IB) and fibroblast growth factor receptor 2 (FGFR2) in bovine follicular cells. Materials and methods. Total RNA was isolated from pooled samples of oocytes (OOs), cumulus cells (CCs) of cumulus oocyte complexes (COCs) and follicular cell pellets (PCs) of 70 ovaries obtained from 96 beef heifers, collected at a local abattoir. The expression pattern of growth factors and their receptors in follicular bovine cells was evaluated by reverse transcriptase polymerase chain reaction (RT-PCR). Results. The mRNA transcripts encoding GDF9, BMP15, FGF2, TGFβ-R1, BMPR-IB and FGFR2 genes were detected, by RT-PCR, in all studied cells. This is the first time that the expression of TGFβ-R1 and BMPR-IB receptors is reported in bovine oocytes. Conclusions. The presence of growth factors and receptor transcripts in the studied cells indicate that these factors could act as paracrine and autocrine regulators of folliculogenesis.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Expresión génica del factor de crecimiento BMP15, GDF9, FGF2 y sus receptores en células foliculares bovinas

Reineri¹,

Coria²,

Barrionuevo³

et al. 2018

Rev MVZ Córdoba

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“…Any cow with follicles reduced in diameter by greater than 2 mm, a sign of follicular fluid leakage, should be removed from the experiment. We have obtained 80-100% ovulation rates in control follicles (saline-injected), which confirms that this procedure does not interfere with the normal ovulation process (Ferreira et al, 2007;Haas et al, 2016).…”

Section: Intrafollicular Injection (Ifi)supporting

confidence: 76%

“…2A). Treatments have also been performed in the future dominant follicle during deviation (Ferreira et al, 2011a;Gasperin et al, 2012) or in differentiated dominant follicles (Lv et al, 2009;Haas et al, 2016). Ultrasound monitoring and ovariectomy allow for exploring the effect of treatment on follicular development, steroidogenesis and gene expression.…”

Section: Follicular Development and Atresiamentioning

confidence: 99%

Methods to study ovarian function in monovulatory species using the cow as a model

Rovani¹,

Gasperin

Ferreira³

et al. 2017

Anim Reprod

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In this review, we discuss the utility of the cow as an in vivo model to study the regulation of ovarian functions in monovular species. It is increasingly becoming clear that besides endocrine control, locally produced factors play pivotal roles during dominant follicle selection, oocyte maturation, ovulation and luteolysis. Although in vitro culture systems have been used to study these processes, definitive understanding the interactions between endocrine and local factors requires appropriate in vivo models. Most of the experimental approaches to study ovarian functions in vivo in large animals are based on the use of ultrasonography and considerable progress in this field has been made during the last thirty years. It has been shown that cows are an excellent model to collect samples (e.g., follicular fluid, granulosa cells, oocytes) from live animals at specific stages of follicular development in order to study mechanisms of intrafollicular factors in a physiological endocrine environment. In addition to support fundamental studies, the cow model has contributed immensely to the refinement of assisted reproductive technologies, which are now widely used not only in farm animals but also in humans.

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“…Both GDF-9 and BMP-15 were described to be involved in the communication between oocytes and the adjacent cumulus cells by regulating amino acid uptake, glycolysis, and cholesterol biosynthesis from cumulus cells via gap junctions to the oocyte [5,9]. In addition to the regulation of cumulus cell metabolism, GDF-9 and BMP-15 regulate diverse processes and gene expression during the preovulatory stage [10].…”

Section: Introductionmentioning

confidence: 99%

GDF-9 and BMP-15 mRNA Levels in Canine Cumulus Cells Related to Cumulus Expansion and the Maturation Process

Ramirez

Palomino

Aspee

et al. 2020

Animals

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The competence to undergo expansion is a characteristic of cumulus cells (CCs). The aim was to investigate the expression of GDF-9 and BMP-15 mRNA in canine cumulus cells in relation to cumulus expansion and meiotic development over the estrous cycle. CCs were recovered from nonmatured and in vitro-matured (IVM) dog cumulus oocyte complexes (COCs), which were obtained from antral follicles at different phases of the estrous cycle. Quantitative real-time polymerase chain reaction (q-PCR) was used to evaluate the relative abundance of GDF-9 and BMP-15 transcripts from the CCs with or without signs of expansion. The results were evaluated by ANOVA and logistic regression. The maturity of the oocyte and the expansion process affected the mRNA levels in CCs. There were differences (p < 0.05) in GDF-9 and BMP-15 gene expression in CCs isolated from nonmatured COCs when comparing the reproductive phases. Lower mRNA levels (p < 0.05) were observed in anestrus and proestrus in comparison to those in estrus and diestrus. In contrast, when comparing GDF-9 mRNA levels in IVM COCs, no differences were found among the phases of the estrous cycle in expanded and nonexpanded CCs (p < 0.05). However, the highest (p < 0.05) BMP-15 gene expression in CCs that did not undergo expansion was exhibited in anestrus and the lowest (p < 0.05) expression was observed in estrus in expanded CCs. Although the stage of the estrous cycle did not affect the second metaphase (MII )rates, the expanded CCs obtained at estrus coexisted with higher percentages of MII (p < 0.05). In conclusion, the differential expression patterns of GDF-9 and BMP-15 mRNA transcripts might be related to cumulus expansion and maturation processes, suggesting specific regulation and temporal changes in their expression.

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Expression of growth and differentiation Factor 9 and cognate receptors during final follicular growth in cattle

Cited by 7 publications

References 21 publications

Expresión génica del factor de crecimiento BMP15, GDF9, FGF2 y sus receptores en células foliculares bovinas

Expresión génica del factor de crecimiento BMP15, GDF9, FGF2 y sus receptores en células foliculares bovinas

Methods to study ovarian function in monovulatory species using the cow as a model

GDF-9 and BMP-15 mRNA Levels in Canine Cumulus Cells Related to Cumulus Expansion and the Maturation Process

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