Expression of Heat Shock Protein 70 in the Periodontal Ligament During Orthodontic Tooth Movement

Kaneko-Tanaka, Keiko; Kurokawa, Akihiko; Mitsuhashi, Masami; Fujita, Shoji; Goseki, Takemi; Yamaguchi, Masaru; Kasai, Kazuhiko

doi:10.5466/ijoms.9.115

Cited by 6 publications

(5 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HSPs play pivotal roles in various conditions both in physiological or pathological cellular response [27]. This study result in line with previous research, which demonstrated that the expression of HSP70 expressed in the late phase after OTM [28]. The expression of HSP70 may function in a homeostatic mechanism to compensate for PDL cell changes occurring during orthodontic force [29].…”

Section: Administration Of Egcg During Otm Can Inhibit Excessive Exprsupporting

confidence: 90%

See 1 more Smart Citation

High Mobility Group Box 1 and Heat Shock Protein-70 Expression Post (-)-Epigallocatechin-3-Gallate in East Java Green Tea Methanolic Extract Administration During Orthodontic Tooth Movement in Wistar Rats

Nugraha

Narmada

Sitasari

et al. 2020

Pesqui. Bras. Odontopediatria Clín. Integr.

View full text Add to dashboard Cite

To investigate the expression of High Mobility Group Box 1 (HMGB1) and Heat Shock Protein-70 (HSP-70) during orthodontic tooth movement (OTM) after (-)-Epigallocatechin-3-Gallate (EGCG) in East Java Green Tea (Camelia Sinensis) Methanolic Extract (GTME) administration in vivo. Material and Methods: 28 Wistar rats (Rattus Novergicus) was used and divided into 4 groups accordingly: K-without EGCG and OTM; K+ with OTM, without EGCG for 14 days; T1with OTM for 14 days and EGCG for 7 days; treatment group 2 (T2) with OTM and EGCG for 14 days. OTM animal model was achieved through the installation of the OTM device by means of NiTi close coil spring with 10g force placed between the first incisor and first maxillary molars. The samples were terminated on Day 14. The pre-maxillary was isolated for the immunohistochemical examination. Analysis of Variance (ANOVA) then continued with Tukey Honest Significant Difference (HSD) (p<0.05) was performed to analyze the data. Results: The highest HMGB1 and HSP-70 expression were found in the K+ group pressure side, meanwhile the lowest HMGB1 and HSP-70 expression were found in K-group tension side in the alveolar bone. There was a significant decrease of HMGB1 and HSP-70 expression in T2 compared to T1 and K+ with significant between groups (p<0.05; p=0.0001). Conclusion: The decreased expression of HMGB1 and HSP-70 in alveolar bone of OTM wistar rats due to post administration of GTME that consisted EGCG.

show abstract

Section: Administration Of Egcg During Otm Can Inhibit Excessive Exprsupporting

confidence: 90%

“…The OTM force can cause oxidative stress in PDL, which activated Heat Shock Factor-1 that can lead to HSP-70 increase in the cytoplasm. This increase resulted in the excretion of HSP-70 from cell [28,30]. Meanwhile, EGCG suppressed the over-expression of HSP 70 [31,32].…”

Section: Administration Of Egcg During Otm Can Inhibit Excessive Exprmentioning

confidence: 97%

High Mobility Group Box 1 and Heat Shock Protein-70 Expression Post (-)-Epigallocatechin-3-Gallate in East Java Green Tea Methanolic Extract Administration During Orthodontic Tooth Movement in Wistar Rats

Nugraha

Narmada

Sitasari

et al. 2020

Pesqui. Bras. Odontopediatria Clín. Integr.

View full text Add to dashboard Cite

show abstract

“…Although the PDL is continuously exposed to mechanical stresses such as compression forces or extension forces during occlusion and chewing [ 10 – 12 ], those stress responses are considered to represent a cellular defense mechanism against environmental disturbances due to the up-regulated expression of HSPs [ 17 ]. These studies demonstrated that ERM cells reduce the functions of hard tissue formation and increase alveolar bone resorption and prevent the PDL from being susceptible to dento-alveolar ankylosis [ 3 , 18 ].…”

Section: Discussionmentioning

confidence: 99%

“…If cells are exposed either to a mild or a moderate stress that is sufficient to up regulate the expression of heat shock proteins (HSPs), they are often able to survive subsequent, otherwise lethal stress stimuli. HSPs can be expressed by all types of cells and they play a protective role against a variety of harmful factors, including oxidants, inflammation, hypoxia, hyperthermia and also mechanical stimuli include orthodontic force [ 10 – 14 ]. Furthermore, strong orthodontic force induced apoptosis of PDL fibroblasts, and many ERM cells also fell into apoptosis [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

HSP70 mRNA expression by cells of the epithelial rest of Malassez due to mechanical forces in vitro

et al. 2016

View full text Add to dashboard Cite

BackgroundThe purpose of the present study was to examine the in vitro responses of ERM cells under the combination of centrifugal and compression forces, in terms of their expression of HSP70 mRNA.MethodsThe ERM cells were positive for CK19 indicating that they were derived from the odontogenic epithelium. Cultured ERM cells were applied centrifugal force and compressing force at one to three times as mechanical forces. After addition of forces, cells were observed using scanning electron microscope (SEM) and were measured expression of HSP70 mRNA by RT-PCR.ResultsSEM observations showed the cells were flattened immediately after the application of mechanical force, but nuclear protrusions recovered the same as the control 3 h later. A significantly higher expression of HSP70 mRNA was observed in ERM cells under mechanical force compared with the control, but it gradually decreased with time. No accumulation of HSP70 mRNA expression occurred with intermittent force. However, the expression of HSP70 mRNA with intermittent force repeated 3 times was significantly higher compared with intermittent force applied only once or twice.ConclusionsThese findings suggest that ERM cells express HSP70 mRNA in response to mechanical force, and that intermittent force maintains the level of HSP70 mRNA expression.

show abstract

“…The result is in accordance with the preceding study which argued that the probiotic therapy for 12 days was able to reduce the number of osteoclasts in the periodontal tissues in the compression area of teeth with mechanic load in mice. 2,24…”

Section: Fig 1: Wistar Rat (R Novergicus) With Otm Appliancementioning

confidence: 99%

The effect of bifidobacterium probiotic on heat shock protein-70 expression and osteoclast number during orthodontic tooth movement in rats (Rattus novergicus)

Triwardhani¹,

Oktaviona²,

Narmada³

et al. 2021

Research Journal of Pharmacy and Technology

View full text Add to dashboard Cite

Heat shock protein (HSP)-70 expressed in the fibroblast of periodontal ligament after orthodontic tooth movement (OTM) was applied. Bifidobacterium probiotics regulate the number of osteoclasts through controlling the inflammation via Nuclear Factor Kappa Beta (NFKB) and increased cytoprotection by HSP induction. This study aimed to examine the effect of Bifidobacterium probiotic on HSP-70 expression and osteoclast number during OTM in the compression side of Wistar rats (Rattus novergicus). This research was a true experimental study which used post-test only group design. 42-healthy-Wistar rats (R. novergicus) were blind randomly divided into 6 groups; K1: OTM for 3 days; K2: OTM for 7 days; K3: OTM for 14 days; KP1: OTM and administration of Bifidobacterium probiotic for 3 days; KP2: OTM and administration of Bifidobacterium probiotic for 7 days; KP3: OTM and administration of Bifidobacterium probiotic for 14 days. OTM model was made by installed closed-coil spring with 10g/mm 2 force. Bifidobacterium probiotic was orally administered with 10mL/day by means of oral gavage at 1.5 x 10 8 CFU/mL dose every day. Immunohistochemical analyses were performed in all study groups. One-way ANOVA and LSD test (p<0.05) were performed to compare between groups. The highest HSP-70 expression was discovered in K3 control group and the greatest osteoclast number was found in KP1 control group. The lowest HSP-70 expression and osteoclast number were found in KP3 group with significant between groups. Bifidobacterium probiotic decreased the expression of HSP-70 and osteoclast number during the orthodontic tooth movement in 14 days in the compression side.

show abstract

Expression of Heat Shock Protein 70 in the Periodontal Ligament During Orthodontic Tooth Movement

Cited by 6 publications

References 23 publications

High Mobility Group Box 1 and Heat Shock Protein-70 Expression Post (-)-Epigallocatechin-3-Gallate in East Java Green Tea Methanolic Extract Administration During Orthodontic Tooth Movement in Wistar Rats

High Mobility Group Box 1 and Heat Shock Protein-70 Expression Post (-)-Epigallocatechin-3-Gallate in East Java Green Tea Methanolic Extract Administration During Orthodontic Tooth Movement in Wistar Rats

HSP70 mRNA expression by cells of the epithelial rest of Malassez due to mechanical forces in vitro

The effect of bifidobacterium probiotic on heat shock protein-70 expression and osteoclast number during orthodontic tooth movement in rats (Rattus novergicus)

Contact Info

Product

Resources

About