Expression of heat shock protein 90 genes during early development and infection in Megalobrama amblycephala and evidence for adaptive evolution in teleost

Ding, Zhujin; Wu, Junjie; Su, Li-Na; Zhang, Fengjuan; Zhao, Xiaoheng; Deng, Wei; Zhang, Jie; Liu, Shikai; Wang, Weimin; Liu, Hong

doi:10.1016/j.dci.2013.08.007

Cited by 17 publications

(6 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The level of expression of SpHsp90 mRNA in the hepatopancreas, spleen, kidney, and blood was significantly increased (P < 0.05) after challenge. A similar Hsp90 pattern of expression was observed in M. amblycephala after A. hydrophila infection (Ding et al 2013). In the current study, the blood, hepatopancreas, and kidney rapidly responded initially (after 0 h), but the spleen reaction was late (after 6 h), and related to organ function and route of bacterial infection.…”

Section: Discussionsupporting

confidence: 67%

Molecular characterization and expression analysis of Hsp90 in Schizothorax prenanti

Zhu

Wang

et al. 2016

Cell Stress and Chaperones

View full text Add to dashboard Cite

Aquatic animals suffer from various environmental stresses because the aquatic environment is a very complex system. To monitor the health status of fish, Hsp90 a potential early warning marker was determined in Schizothorax prenanti after infection with a bacterium. In this study, we cloned Hsp90 from S. prenanti for the first time. The full-length cDNA sequence of SpHsp90 was 2663 bp, contains an open reading frame of 2181 bp, and has a gene encoding 726 amino acids, an estimated molecular mass of 83.38 kDa, and a theoretical isoelectric point of 4.91. The SpHsp90 amino acid sequence has five conserved HSP90 family signatures and shares 87.0-95.5 % identity with other vertebrates. Phylogenetic analysis and structure comparison indicated that SpHsp90 should be a β isoform of the HSP90 family. SpHsp90 was ubiquitously expressed in all examined tissues, and the highest level of expression was in the kidney. After Streptococcus agalactiae infection, the level of SpHsp90 expression had significant changes (P < 0.05) in the hepatopancreas, spleen, kidney, and blood. The expression increased to the highest level at 6 h in the blood and at 24 h in the hepatopancreas, spleen, and kidney. The results suggested that the SpHsp90 gene could be induced by S. agalactiae in S. prenanti and that SpHsp90 may be involved in resistance to bacterial infection and provide an early warning information. The kidney is the most suitable for detecting SpHsp90 after bacterial infection.

show abstract

Section: Discussionsupporting

confidence: 67%

Molecular characterization and expression analysis of Hsp90 in Schizothorax prenanti

Zhu

Wang

et al. 2016

Cell Stress and Chaperones

View full text Add to dashboard Cite

show abstract

“…Previous studies in our lab indicated that some immune-related genes are activated at an early stage of A. hydrophila infection in M. amblycephala [31,32,33,34,35]. Therefore, three small RNA libraries were analyzed from M. amblycephala liver at 0 (non-infection), 4, and 24 h post infection (poi) using the Illumina Hiseq 2000 sequencing platform.…”

Section: Resultsmentioning

confidence: 99%

Identification and Characterization of MicroRNAs in the Liver of Blunt Snout Bream (Megalobrama amblycephala) Infected by Aeromonas hydrophila

Cui

Wei

et al. 2016

IJMS

Self Cite

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are small RNA molecules that play key roles in regulation of various biological processes. In order to better understand the biological significance of miRNAs in the context of Aeromonas hydrophila infection in Megalobrama amblycephala, small RNA libraries obtained from fish liver at 0 (non-infection), 4, and 24 h post infection (poi) were sequenced using Illumina deep sequencing technology. A total of 11,244,207, 9,212,958, and 7,939,157 clean reads were obtained from these three RNA libraries, respectively. Bioinformatics analysis identified 171 conserved miRNAs and 62 putative novel miRNAs. The existence of ten randomly selected novel miRNAs was validated by RT-PCR. Pairwise comparison suggested that 61 and 44 miRNAs were differentially expressed at 4 and 24 h poi, respectively. Furthermore, the expression profiles of nine randomly selected miRNAs were validated by qRT-PCR. MicroRNA target prediction, gene ontology (GO) annotation, and Kyoto Encylopedia of Genes and Genomes (KEGG) analysis indicated that a variety of biological pathways could be affected by A. hydrophila infection. Additionally, transferrin (TF) and transferrin receptor (TFR) genes were confirmed to be direct targets of miR-375. These results will expand our knowledge of the role of miRNAs in the immune response of M. amblycephala to A. hydrophila infection, and facilitate the development of effective strategies against A. hydrophila infection in M. amblycephala.

show abstract

“…Specifically, Amhsp70 and Amhsc70 were expressed at high levels in liver, and Amhsp90 mRNA expression was the highest level in heart. In comparison with M. amblycephala, S. senegalensis, S. ocellatus and O. niloticus , there were some different expression patterns with tissue-specific distribution [26–29]. Therefore, the distribution of hsp genes is varied in different tissues owing to different species.…”

Section: Discussionmentioning

confidence: 99%

The role of three heat shock protein genes in the immune response to Aeromonas hydrophila challenge in marbled eel, Anguilla marmorata

et al. 2016

View full text Add to dashboard Cite

Heat shock proteins (HSPs) are highly conserved molecular chaperones that play critical roles in both innate and adaptive immunity. However, little information about HSPs from marbled eel Anguilla marmorata is known. In this study, the full-length Amhsp90 (2527 bp), Amhsp70 (2443 bp) and Amhsc70 (2247 bp) were first cloned from A. marmorata, using rapid amplification of cDNA ends, containing open reading frames of 2181, 1932 and 1950 bp in length, and encoding proteins with 726, 643 and 649 amino acids, respectively. The deduced amino acid sequences of three Amhsps shared a high homology similarity with other migratory fish. Real-time fluorescent quantitative polymerase chain reaction was used to evaluate tissue-specific distribution and mRNA expression levels of three Amhsps subjected to infection with Aeromonas hydrophila. The mRNA expression of three Amhsps in eight tested tissues, namely liver, heart, muscle, gill, spleen, kidney, brain and intestine, of juvenile A. marmorata was evaluated to reveal the major expression distribution in liver, intestine, muscle and heart. After pathogen challenge treatments, mRNA transcriptions of three Amhsps revealed a significant regulation at various time points in the same tissue. All these findings suggest that Amhsps may be involved in the immune response in A. marmorata.

show abstract

Expression of heat shock protein 90 genes during early development and infection in Megalobrama amblycephala and evidence for adaptive evolution in teleost

Cited by 17 publications

References 59 publications

Molecular characterization and expression analysis of Hsp90 in Schizothorax prenanti

Molecular characterization and expression analysis of Hsp90 in Schizothorax prenanti

Identification and Characterization of MicroRNAs in the Liver of Blunt Snout Bream (Megalobrama amblycephala) Infected by Aeromonas hydrophila

The role of three heat shock protein genes in the immune response to Aeromonas hydrophila challenge in marbled eel, Anguilla marmorata

Contact Info

Product

Resources

About