Expression of heat shook protein 27 and proliferating cell nuclear antigen in human retinoblastoma

Abstract

Aim of the studyThis study aimed to observe the expressions of heat shock protein 27 (HSP27) and proliferating cell nuclear antigen (PCNA) in retinoblastoma (Rb) cells and to explore the relationships of the expression with Rb differentiation and optic nerve infiltration.Material and methodsHeat shock protein 27 and PCNA expressions in 36 routine Rb paraffin specimens were observed using PV9000 two-stage immunohistochemical staining. The correlations of the HSP27 and PCNA expressions with Rb differentiation and optic nerve infiltration were analyzed.ResultsHeat shock protein 27 was weakly expressed in the normal retina, specifically in the ganglion cell layer. It was extensively expressed in Rb tissues at a positive rate of 69.4%, and the positive substances were primarily located in the cytoplasm. Proliferating cell nuclear antigen was expressed weakly or not at all expressed in the normal retina and was extensively expressed in Rb tissues at a positive rate of 83.3%, and the positive substances were primarily located in the nucleus. The positive expression rates of HSP27 and PCNA in the differentiated group were significantly higher than in the undifferentiated group (p < 0.05). The positive expression rates of HSP27 and PCNA in the optic nerve-infiltrated group were significantly higher than in the non-infiltrated group (p < 0.05). Heat shock protein 27 expression was positively correlated with PCNA expression in Rb (p < 0.01).ConclusionsHeat shock protein 27 and PCNA expressions are markedly correlated with cell differentiation and optic nerve infiltration in Rb.

Zinc: What Is Its Role in Lung Cancer?

“…Likewise, Hsp70 has a role in iNOS induction [16,31,33]. In addition, proliferating cell nuclear antigen (PCNA) expression was increased in the ZD group [31,34]. Qin and colleagues [31] demonstrated a possible association between PCNA and Hsp27 expression in retinoblastoma tumor.…”

“…Heat shock proteins are chaperones that play a pivotal role in cells survival under stressful conditions. Under normal conditions, Heat shock protein 27(Hsp27) is weakly expressed in cells; however, once stress occurs, Hsp27 expression increases, exerting an anti-oxidative damage function [31]. We analyzed the effect of Zn deficiency on the expression of cytoprotective factors (Hsp27 and Hsp70i) where both chaperones increased and are consistently associated with cellular stress and inflammation in lung [32,33].…”

“…We analyzed the effect of Zn deficiency on the expression of cytoprotective factors (Hsp27 and Hsp70i) where both chaperones increased and are consistently associated with cellular stress and inflammation in lung [32,33]. Likewise, Hsp70 has a role in iNOS induction [16,31,33]. In addition, proliferating cell nuclear antigen (PCNA) expression was increased in the ZD group [31,34].…”

“…Qin and colleagues [31] demonstrated a possible association between PCNA and Hsp27 expression in retinoblastoma tumor. There is a lot of evidence that PCNA expression can be used as an index for evaluating malignant tumor cells proliferation, as well as the malignant potential and prognosis of a tumor [31,35,36].…”