Expression of HSP72 in the gastric mucosa is regulated by gastric acid in rats—Correlation of HSP72 expression with mucosal protection

Wada, Isao; Otaka, Michiro; Jin, Mario; Odashima, Masaru; Komatsu, Kyoji; Konishi, Noriaki; Matsuhashi, Tamotsu; Horikawa, Youhei; Ohba, Reina; Itoh, Hidenori; Watanabe, Sumio

doi:10.1016/j.bbrc.2006.08.088

Cited by 47 publications

(32 citation statements)

References 35 publications

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“…The expression of Hsp70 is induced by several endogenous physiological or environmental stressors, such as heat, ethanol, caloric restriction, and (Musch et al 1999;Ren et al 2001;Wada et al 2006). Hsp70 helps to maintain cells integrity in normal conditions and prevent and repair damages induced in cells and organs by various stresses.…”

Section: Discussionmentioning

confidence: 99%

“…The apoptosis rate of intestinal epithelia inversely correlates with villus height and crypt depth and is accelerated by oxidative stress (Marshman et al 2001). Many studies have shown that the expression of Hsp70 was induced to prevent apoptosis and necrosis by gastrointestinal lesion of stress (Oyake et al 2006;Wada et al 2006). In addition, it has been reported that food restriction can affect Hsp70 expression in rats (Ahmad et al 1995).…”

Section: Discussionmentioning

confidence: 99%

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Heat shock protein 70 is upregulated in the intestine of intrauterine growth retardation piglets

Zhong

Wang

Zhang

et al. 2010

Cell Stress and Chaperones

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The objective of this study is to investigate the expression and distribution of heat shock protein 70 (Hsp70) in the intestine of intrauterine growth retardation (IUGR) piglets. Samples from the duodenum, prejejunum, distal jejunum, ileum, and colon of IUGR and normalbody-weight (NBW) piglets were collected at birth. The results indicated that the body and intestine weight of IUGR piglets were significantly lower than NBW piglets. The villus height and villus/crypt ratio in jejunum and ileum of IUGR piglets were significantly reduced compared to NBW piglets. These results indicated that IUGR causes abnormal gastrointestinal morphologies and gastrointestinal dysfunction. The mRNA of hsp70 was increased in prejejunum (P<0.05), distal jejunum (P<0.05), and colon in IUGR piglets. However, the hsp70 mRNA in ileum of piglets with IUGR was decreased. Similar to hsp70 mRNA, the protein levels of Hsp70 in prejejunum (P<0.05), distal jejunum, and colon (P<0.05) in IUGR piglets were higher than those in NBW piglets. These results indicated that the expression of Hsp70 in the intestinal piglets was upregulated by IUGR, and different intestinal sites had different responses to stress. Meanwhile, the localization of Hsp70 in the epithelial cells of the whole villi and intestinal gland rather than in the lamina propria and myenteron suggested that Hsp70 has a cytoprotective role in epithelial cell function and structure.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Heat shock protein 70 is upregulated in the intestine of intrauterine growth retardation piglets

Zhong

Wang

Zhang

et al. 2010

Cell Stress and Chaperones

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“…Proton pump inhibitors (PPI) also induce HO-1 in endothelial and gastric epithelial cells (34). However, treatment with gastric acid-suppressing agents such as PPI and H 2 -receptor antagonists reduced the expression of HSP70 in the gastric mucosa and the gastric mucosa was vulnerable to HCl-induced AGML, while co-administration with PZ restored the resistance against HCl-induced AGML (35). Our data indicated that PZ was effective for preventing the irritant-induced AGML by a combination of multiple mechanisms, at least with induction of HO-1 as well as HSP70.…”

Section: Discussionmentioning

confidence: 99%

Polaprezinc (Zinc L-Carnosine) Is a Potent Inducer of Anti-oxidative Stress Enzyme, Heme Oxygenase (HO)-1 — a New Mechanism of Gastric Mucosal Protection

et al. 2009

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Abstract. Heme oxygenase (HO)-1 is implicated in cytoprotection in various organs. We tested a possibility that polaprezinc (PZ), an anti-ulcer drug, could induce HO-1 in the gastric mucosa. Male 6-week-old Wistar rats were intragastrically administered PZ. Gastric expression of HO-1 was assessed by real time RT-PCR and western blotting, and localization of HO-1 was observed by in situ hybridization and immunohistochemistry. The levels of HO-1 mRNA were increased in a dose-dependent manner. The levels of HO-1 mRNA were increased 4-fold by PZ at the dose of 200 mg/ kg at 3 h as compared with control levels. The levels of immunoreactive HO-1 were increased 3-fold at 6 h. Signals for HO-1 mRNA and immunoreactivity were detected strongly in the surface gastric mucosal cells and moderately in the gastric macrophages. Treatment with an HO-1 inhibitor, stannous mesoporphyrin (SnMP) significantly worsened the HCl-induced acute gastric mucosal lesions and increased the apoptosis of mucosal cells. Mucosal lesions were decreased by pretreatment with PZ, while they were increased by co-administration with SnMP. These data indicate for the first time that PZ is an effective inducer of HO-1 in the stomach. PZinduced HO-1 functions as a part of the mucosal protective effects of PZ.

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“…It is widely accepted that the HSP70 family has important functions in the gastric mucosal protection although HSP60 has no protective ability [11,17,[27][28][29][30]. On the other hand, in the pancreas, HSP60 has been reported to have a crucial role in cytoprotection whereas HSP70 does not contribute [12].…”

Section: Discussionmentioning

confidence: 99%

“…These functions are considered to be important for living cells to obtain tolerance to adapt to environmental changes [8,9]. Also in the gastrointestinal mucosa, cytoprotective functions of HSP70 and HSP60 have been demonstrated in several experimental models [10][11][12][13][14][15][16][17]. Although HSP90 is a molecular chaperone that is ubiquitously expressed in living cells and plays an important role in cell growth and differentiation, no precise research has been performed to clarify the functional role of HSP90 in the intestinal mucosal cells.…”

Section: Introductionmentioning

confidence: 99%

Evidence for Enhanced Cytoprotective Function of HSP90-Overexpressing Small Intestinal Epithelial Cells

et al. 2011

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Expression of HSP72 in the gastric mucosa is regulated by gastric acid in rats—Correlation of HSP72 expression with mucosal protection

Cited by 47 publications

References 35 publications

Heat shock protein 70 is upregulated in the intestine of intrauterine growth retardation piglets

Heat shock protein 70 is upregulated in the intestine of intrauterine growth retardation piglets

Polaprezinc (Zinc L-Carnosine) Is a Potent Inducer of Anti-oxidative Stress Enzyme, Heme Oxygenase (HO)-1 — a New Mechanism of Gastric Mucosal Protection

Evidence for Enhanced Cytoprotective Function of HSP90-Overexpressing Small Intestinal Epithelial Cells

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