EXPRESSION OF HUMAN IMMUNODEFICIENCY VIRUS ANTIGEN (HIV-Ag) IN SERUM AND CEREBROSPINAL FLUID DURING ACUTE AND CHRONIC INFECTION

Goudsmit, Jaap; Paul, Deborah A.; Lange, J. J. De; Speelman, Hans; Noordaa, J. van der; Helm, HayoJ. Van Der; Wolf, Frank de; Epstein, Leon G.; Krone, WillyJ.A.; Wolters, E. C.; Oleske, James M.; Coutinho, R. A.

doi:10.1016/s0140-6736(86)92485-2

Cited by 494 publications

(146 citation statements)

References 17 publications

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“…Cell culture supernatants were assayed at 3-day intervals for HIV antigen (HIV antigen assay, Abbott Laboratories) (Goudsmit et al, 1986;Newell et al, 1987); this assay detects the HIV p24 protein. We determined that the HIV p24 protein concentration (pg/ml) was proportional to the optical density (OD) units in the ELISA, and the cutoff(threshold) in OD units corresponded to a limit of detection of HIV p24 of 9 pg/ml.…”

Section: Detection and Quantification Of Hiv Infectionmentioning

confidence: 99%

Recombinant interleukin 4 stimulates human immunodeficiency virus production by infected monocytes and macrophages

Kazazi

Mathijs²,

Chang³

et al. 1992

Journal of General Virology

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Section: Detection and Quantification Of Hiv Infectionmentioning

confidence: 99%

Recombinant interleukin 4 stimulates human immunodeficiency virus production by infected monocytes and macrophages

Kazazi

Mathijs²,

Chang³

et al. 1992

Journal of General Virology

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“…Cell culture supernatants were assayed at 3 day intervals for HIV antigen (HIV antigen assay; Abbott Laboratories) (Goudsmit et al, 1986;Newell et al, 1987). This assay detects predominantly the HIV p24 protein.…”

Section: Introductionmentioning

confidence: 99%

Variations in CD4 Expression by Human Monocytes and Macrophages and Their Relationship to Infection with the Human Immunodeficiency Virus

Kazazi

Mathijs

Foley

et al. 1989

Journal of General Virology

135

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SUMMARYThe expression of CD4 antigen on the surface of LeuM3-positive human blood monocytes was found to be variable with 65 to 90~ of cells from 46 normal human volunteers being positive by dual staining flow cytometry. When monocytes adhered to plastic (but not when cultured on Teflon), a marked decrease in CD4 expression was observed between 1 and 24 h post-adherence. CD4 expression could not be detected in macrophages adhered to plastic for 5 days by using four anti-CD4 monoclonal antibodies in flow cytometry or direct immunofluorescence. Conversely an increasing proportion of adherent cells expressed LeuM3 and OKM5 surface antigens over the 5 days. CD4 mRNA levels were measured by slot-blot and Northern hybridization, and total cellular CD4 protein levels by immunoprecipitation. Both cellular mRNA and 'CD4 levels remained constant throughout the 5 day period but membrane CD4 protein levels were greatly reduced indicating that the down-regulation of CD4 was posttranslational. Infection with two of six fresh human immunodeficiency virus (HIV) isolates showed different kinetic patterns when tested on purified monocytes recently adhered to plastic and macrophages adherent for 5 days. HIV antigen and reverse transcriptase levels in infected monocyte cultures remained high for 3 to 4 weeks before detachment and necrosis of the cells occurred. Infection of macrophages generated much lower levels of antigen and reverse transcriptase which declined to very low or undetectable levels over 2 weeks, leaving persisting viable macrophages. One week after infection HIV nucleic acid was detected in 69 + 7~ of monocytes and 6 + 3~ of macrophages by in situ hybridization. Blocking experiments with anti-Leu3a monoclonal antibody suggested that HIV infection of 5 day adherent macrophages occurred mainly by a mechanism other than binding to CD4.

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“…Soon after infection, when neutralizing antibodies appear, free virus antigens disappear from the serum (Goudsmit et al, 1986). After an asymptomatic period of variable duration, HIV-1 antigenaemia may reappear even in the presence of high neutralizing antibody activity (Goudsmit et al, 1988d).…”

Section: Introductionmentioning

confidence: 99%

Specificity and Function of the Individual Amino Acids of an Important Determinant of Human Immunodeficiency Virus Type 1 that Induces Neutralizing Activity

Meloen

Liskamp²,

Goudsmit

1989

Journal of General Virology

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EXPRESSION OF HUMAN IMMUNODEFICIENCY VIRUS ANTIGEN (HIV-Ag) IN SERUM AND CEREBROSPINAL FLUID DURING ACUTE AND CHRONIC INFECTION

Cited by 494 publications

References 17 publications

Recombinant interleukin 4 stimulates human immunodeficiency virus production by infected monocytes and macrophages

Recombinant interleukin 4 stimulates human immunodeficiency virus production by infected monocytes and macrophages

Variations in CD4 Expression by Human Monocytes and Macrophages and Their Relationship to Infection with the Human Immunodeficiency Virus

Specificity and Function of the Individual Amino Acids of an Important Determinant of Human Immunodeficiency Virus Type 1 that Induces Neutralizing Activity

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