Expression of Human Scavenger Receptor B1 on and in Human Platelets

Imachi, Hitomi; Murao, Koji; Cao, Wen-Ming; Tada, Satoshi; Taminato, Tomohiko; Wong, Norman C.W.; Takahara, Jiro; Ishida, Toshihiko

doi:10.1161/01.atv.0000067429.46333.7b

Cited by 68 publications

(61 citation statements)

References 45 publications

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“…HDL has also been shown to have antithrombotic effects. Platelets express the full repertoire of HDL/apoA-I receptors (including ABCA1 [152] and SR-B1 [187]) and interact with HDL and apoA-I [188][189][190]. HDL can inhibit thrombin induced platelet aggregation and fibrinogen binding [151].…”

Section: 13mentioning

confidence: 99%

High Density Lipoprotein: Assembly, Structure, Cargo, and Functions

Murphy

2013

ISRN Physiology

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Cardiovascular disease (CVD) is the leading cause of death globally. For close to four decades, we have known that high density lipoprotein (HDL) levels are inversely correlated with the risk of CVD. HDL is a complex particle that consists of proteins, phospholipids, and cholesterol and has the ability to carry micro-RNAs. HDL is constantly undergoing remodelling throughout its life-span and carries out many functions. This review summarizes many of the different aspects of HDL from its assembly, the receptors it interacts with, along with the functions it performs and how it can be altered in disease. While HDL is a key cholesterol efflux particle, this review highlights the many other important functions of HDL in the innate immune system and details the potential therapeutic uses of HDL outside of CVD.

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Section: 13mentioning

confidence: 99%

High Density Lipoprotein: Assembly, Structure, Cargo, and Functions

Murphy

2013

ISRN Physiology

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“…4 Our previous reports show that human homologue of SR-BI, CD36, and LIMPII analogous-1 (hSR-BI/CLA-1), like mouse SR-BI, functions as a receptor for HDL. 5,6 However, the mechanisms by which HDL is atheroprotective are complex and not well understood.Endothelial dysfunction plays a pivotal role in the initiation and progression of atherosclerosis. In fact, the vascular endothelium modulates the vessel tone by releasing both relaxing and contractile factors, 7 regulates the adherence of mononuclear cells to its surface and the vascular permeability, 8,9 and produces substances involved in the regulation of hemostasis and tissue proliferation.…”

mentioning

confidence: 99%

Regulation of Scavenger Receptor Class BI Gene Expression by Angiotensin II in Vascular Endothelial Cells

Murao

Imachi

et al. 2007

Hypertension

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Abstract-High-density lipoprotein mediates a normal physiological process called reverse cholesterol transport. In this process, a scavenger receptor of the B class (SR-BI)/human homologue of SR-BI, CD36, and LIMPII analogous-1 (hSR-BI/CLA-1) facilitates the cellular uptake of cholesterol from high-density lipoprotein. 1,2 In this process, HDL particles shuttle cholesterol from extrahepatic tissues to the liver for further metabolism and excretion. 1 Enhanced reverse cholesterol transport lowers total body cholesterol and thereby reduces the risk of developing coronary artery disease. Thus, the plasma concentration of HDL is inversely related to the incidence of coronary artery disease. 3 The mouse scavenger receptor class BI (SR-BI) mediates selective uptake of HDL cholesterol ester into transfected Chinese hamster ovary cells. This finding provides an important link between a specific cell surface receptor and a pathway for the uptake of HDL. 4 Our previous reports show that human homologue of SR-BI, CD36, and LIMPII analogous-1 (hSR-BI/CLA-1), like mouse SR-BI, functions as a receptor for HDL. 5,6 However, the mechanisms by which HDL is atheroprotective are complex and not well understood.Endothelial dysfunction plays a pivotal role in the initiation and progression of atherosclerosis. In fact, the vascular endothelium modulates the vessel tone by releasing both relaxing and contractile factors, 7 regulates the adherence of mononuclear cells to its surface and the vascular permeability, 8,9 and produces substances involved in the regulation of hemostasis and tissue proliferation. 10 The release of NO by endothelial NO synthase (eNOS) is important in the regulation of cardiovascular homeostasis. Recent reports indicated that SR-BI and eNOS are colocalized in vascular endothelial cells. Thus, HDL activates eNOS via SR-BI, and the resulting

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“…5 Using this method, we found a significant increase in the cholesterol ester (CE) content in platelets from patients with atherosclerotic disease. 6 Although this technique is fully automated and reliable, the use of this assay has limitations due to the need for special equipment and its ability to perform only a confined menu of tests. It is therefore difficult to apply this assay for the routine determination of cellular cholesterol.…”

Section: Introductionmentioning

confidence: 99%

Automated assay for determining cellular cholesterol using a random access chemistry analyser

Tada¹,

Kitanaka²,

Kubota

et al. 2010

Ann Clin Biochem

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Background: We have previously reported an ultrasensitive fluorometric assay for measuring cellular cholesterol. Although this technique is reliable, the use of the assay has limitations due to the requirement for special equipment. It is therefore difficult to apply this assay for the routine determination of cellular cholesterol. Methods: A colorimetric assay to measure cellular cholesterol was established that utilizes reagents widely used for the measurement of cholesterol in blood samples in conjunction with a random access chemistry analyser ARCHITECT c8000 that is also common in clinical laboratories. Results: This colorimetric assay showed excellent linearity and recovery. The within-run coefficients of variation were less than 2.5%. The sensitivity of this method, with its detection limit of 1.29 mmol/L, was found to be superior to that of the fluorometric assay we have developed previously. In platelets obtained from patients with diabetes, both the free cholesterol and cholesterol ester content were significantly increased. Conclusions: Using this technique, measurement of cellular cholesterol could be performed routinely without the requirement for special reagents and equipment.

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Expression of Human Scavenger Receptor B1 on and in Human Platelets

Cited by 68 publications

References 45 publications

High Density Lipoprotein: Assembly, Structure, Cargo, and Functions

High Density Lipoprotein: Assembly, Structure, Cargo, and Functions

Regulation of Scavenger Receptor Class BI Gene Expression by Angiotensin II in Vascular Endothelial Cells

Automated assay for determining cellular cholesterol using a random access chemistry analyser

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