Expression of hunchback protein in a subset of ectodermal teloblasts of the oligochaete annelid Tubifex

Shimizu, Takashi; Savage, Robert M.

doi:10.1007/s00427-002-0278-4

Cited by 17 publications

(8 citation statements)

References 11 publications

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“…Although important differences have been highlighted in the roles of hunchback in segmentation (Liu and Kaufman 2004a;Mito et al 2005), hunchback appears to play a role in segmental specialization in all insects studied to date. In contrast, data from Caenorhabditis elegans and annelids suggest that hunchback is unlikely to play any role in segmental/axial patterning in these organisms (Fay et al 1999;Savage and Shankland 1996;Iwasa et al 2000;Werbrock et al 2001;Shimizu and Savage 2002).…”

Section: Introductionmentioning

confidence: 74%

Expression of hunchback during trunk segmentation in the branchiopod crustacean Artemia franciscana

2005

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Comparative studies have shown that some aspects of segmentation are widely conserved among arthropods. Yet, it is still unclear whether the molecular prepatterns that are required for segmentation in Drosophila are likely to be similarly conserved in other arthropod groups. Homologues of the Drosophila gap genes, like hunchback, show regionally restricted expression patterns during the early phases of segmentation in diverse insects, but their expression patterns in other arthropod groups are not yet known. Here, we report the cloning of a hunchback orthologue from the crustacean Artemia franciscana and its expression during the formation of trunk segments. Artemia hunchback is expressed in a series of segmental stripes that correspond to individual thoracic/trunk, genital, and postgenital segments. However, this expression is not associated with the segmenting ectoderm but is restricted to mesodermal cells that associate with the ectoderm in a regular metameric pattern. All cells in the early segmental mesoderm appear to express hunchback. Later, mesodermal expression fades, and a complex expression pattern appears in the central nervous system (CNS), which is comparable to hunchback expression in the CNS of insects. No regionally restricted expression, reminiscent of gap gene expression, is observed during trunk segmentation. These patterns suggest that the expression patterns of hunchback in the mesoderm and in the CNS are likely to be ancient and conserved among crustaceans and insects. In contrast, we find no evidence for a conserved role of hunchback in axial patterning in the trunk ectoderm.

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Section: Introductionmentioning

confidence: 74%

Expression of hunchback during trunk segmentation in the branchiopod crustacean Artemia franciscana

2005

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“…Immunocytochemical whole-mounts of fixed embryos (stages 1-17) were prepared according to Shimizu (1993) and Shimizu and Savage (2002), with some modifications.…”

Section: Whole-mount Immunocytochemistrymentioning

confidence: 99%

Transient occurrence of vasa-expressing cells in nongenital segments during embryonic development in the oligochaete annelid Tubifex tubifex

Oyama

Shimizu

2007

Dev Genes Evol

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“…Furthermore, hbl-1 is also expressed in many neurones, as well as in the pharynx (Fay et al 1999). In addition to arthropods and nematodes that belong to the ecdysozoans, hb orthologs have been isolated in annelids, the leech Helobdella (Iwasa et al 2000;Savage and Shankland 1996), the polychaete Capitella (Werbrock et al 2001) and the oligochaete Tubifex (Shimizu and Savage 2002). In these species, hb is expressed in a subset of neurones of the ventral nerve cord, as well as in a very broad domain comprising ectodermal and endodermal cells.…”

Section: Introductionmentioning

confidence: 98%

The expression of a hunchback ortholog in the polychaete annelid Platynereis dumerilii suggests an ancestral role in mesoderm development and neurogenesis

et al. 2006

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Orthologs of the Drosophila gap gene hunchback have been isolated so far only in protostomes. Phylogenetic analysis of recently available genomic data allowed us to confirm that hunchback genes are widely found in protostomes (both lophotrochozoans and ecdysozoans). In contrast, no unequivocal hunchback gene can be found in the genomes of deuterostomes and non-bilaterians. We cloned hunchback in the marine polychaete annelid Platynereis dumerilii and analysed its expression during development. In this species, hunchback displays an expression pattern indicative of a role in mesoderm formation and neurogenesis, and similar to the expression found for hunchback genes in arthropods. These data suggest altogether that these functions are ancestral to protostomes.

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Expression of hunchback protein in a subset of ectodermal teloblasts of the oligochaete annelid Tubifex

Cited by 17 publications

References 11 publications

Expression of hunchback during trunk segmentation in the branchiopod crustacean Artemia franciscana

Expression of hunchback during trunk segmentation in the branchiopod crustacean Artemia franciscana

Transient occurrence of vasa-expressing cells in nongenital segments during embryonic development in the oligochaete annelid Tubifex tubifex

The expression of a hunchback ortholog in the polychaete annelid Platynereis dumerilii suggests an ancestral role in mesoderm development and neurogenesis

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