Expression of hyaluronan synthases and hyaluronidases in the MG63 osteoblast cell line

Adams, Julian; Sander, Guy R.; Byers, Sharon

doi:10.1016/j.matbio.2005.08.007

Cited by 14 publications

(10 citation statements)

References 54 publications

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“…This was not consistent with a study reporting no change in Has2 expression in mineralizing MG63 cells. 37 Early expression level of bone differentiation related genes such as cbfa1 and ALP gene was decreased on day 7 and increased on day 14, which is different from previous results that found that HA inhibited ALP activity on day 2 and increased it on day 7. 24 This difference may be due to the different isolation methods of BMSC used.…”

Section: Discussioncontrasting

confidence: 93%

Effect of hyaluronan on osteogenic differentiation of porcine bone marrow stromal cells in vitro

Zou

Chen

et al. 2007

Journal Orthopaedic Research

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Hyaluronan (HA) plays a predominant role in tissue morphogenesis, cell migration, proliferation, and cell differentiation. The aims of the present study were to investigate whether (i) prolonged presence of high concentration (4.0 mg/mL) 800 KDa HA and (ii) pretreatment with HA can modify osteogenic differentiation of pig bone marrow stromal cells (pBMSC). Cell proliferation and mineralization were measured. Expression of differentiation-related genes was evaluated by means of real-time reverse transcription polymerase chain reaction (RT-PCR). HA increased cell proliferation on day 7. HA decreased the basal level of bone-related gene expression and increased the basal level of sox9 marginally during 7-day pretreatment with HA. HA increased calcium deposit on day 21. cbfa1, ALP, and type 1a collagen (Col1) expression was increased when pBMSC were cultivated in osteogenic medium, whereas their expression was decreased in the presence of HA on day 7. On day 14, the addition of HA upregulated cbfa1 and ALP expression compared to osteogenic medium group; there was no significant difference in Col1 expression. At day 21, osteocalcin (OC) expression showed 2.5-fold upregulation over osteogenic medium. These results suggest that exogenous HA stimulates endogenous HA, which together may play a synergetic role in osteogenic differentiation under osteoinducing conditions although gene expression was inhibited at the early stage. ß

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Section: Discussioncontrasting

confidence: 93%

Effect of hyaluronan on osteogenic differentiation of porcine bone marrow stromal cells in vitro

Zou

Chen

et al. 2007

Journal Orthopaedic Research

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“…HAS2 knockdown in vitro disrupts HA accumulation, coat formation, cell motility and cell invasiveness [188]. HAS2 expression is not altered during mineralisation in the MG-63 cell line and the observed decrease in HA production corresponds to a decrease in HAS3 expression [187]. Thus, endogenous HA synthesis may restrict mineralisation, and its regulation at the level of the HAS is important for the modulation of mineral deposition during osteogenesis.…”

Section: Effects Of Ha On Bone Remodellingmentioning

confidence: 99%

“…High M r HA increases osteoblast proliferation and mineralisation, whereas low M r HA (60 kDa) increases proliferation but not mineralisation [142]. HA synthesis by osteoblasts is mediated by HAS2 and HAS3 [187,188]. HAS2 knockdown in vitro disrupts HA accumulation, coat formation, cell motility and cell invasiveness [188].…”

Section: Effects Of Ha On Bone Remodellingmentioning

confidence: 99%

“…At the time, the different mammalian HYALs were unknown, but it is now clear that osteoblasts express HYAL2, HYAL3 and HYAL4 [187,197] and are distinct from most other cells for their lack of HYAL1 expression. HYAL activity in osteoclasts has not been characterised.…”

Section: Hyal In Bonementioning

confidence: 99%

“…HYAL activity in osteoclasts has not been characterised. HYAL gene expression is increased during mineralisation: HYAL2 increases by 62-fold, HYAL3 by 13-fold and HYAL4 expression increasing by 3-fold [187]. Since HYAL2 is thought to degrade HA tõ 20-kDa intermediates, increased HYAL2 expression might produce intermediate-sized HA fragments with potential roles in intracellular signalling during mineralisation to promote bone formation [141,198].…”

Section: Hyal In Bonementioning

confidence: 99%

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Hyaluronan synthesis and degradation in cartilage and bone

Bastow

Byers

Golub

et al. 2007

Cell. Mol. Life Sci.

179

106

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Hyaluronan (HA) is a large but simple glycosaminoglycan composed of repeating D-glucuronic acid, beta1-3 linked to N-acetyl-D-glucosamine beta1-4, found in body fluids and tissues, in both intra- and extracellular compartments. Despite its structural simplicity, HA has diverse functions in skeletal biology. In development, HA-rich matrices facilitate migration and condensation of mesenchymal cells, and HA participates in joint cavity formation and longitudinal bone growth. In adult cartilage, HA binding to aggrecan immobilises aggrecan, retaining it at the high concentrations required for compressive resilience. HA also appears to regulate bone remodelling by controlling osteoclast, osteoblast and osteocyte behaviour. The functions of HA depend on its intrinsic properties, which in turn rely on the degree of polymerisation by HA synthases, depolymerisation by hyaluronidases, and interactions with HA-binding proteins. HA synthesis and degradation are closely regulated in skeletal tissues and aberrant synthetic or degradative activity causes disease. The role and regulation of HA synthesis and degradation in cartilage, bone and skeletal development is discussed.

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Hyaluronan-based heparin-incorporated hydrogels for generation of axially vascularized bioartificial bone tissues: in vitro and in vivo evaluation in a PLDLLA–TCP–PCL-composite system

Rath

Pryymachuk²,

Bleiziffer³

et al. 2011

J Mater Sci: Mater Med

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Smart matrices are required in bone tissue-engineered grafts that provide an optimal environment for cells and retain osteo-inductive factors for sustained biological activity. We hypothesized that a slow-degrading heparin-incorporated hyaluronan (HA) hydrogel can preserve BMP-2; while an arterio-venous (A-V) loop can support axial vascularization to provide nutrition for a bio-artificial bone graft. HA was evaluated for osteoblast growth and BMP-2 release. Porous PLDLLA-TCP-PCL scaffolds were produced by rapid prototyping technology and applied in vivo along with HA-hydrogel, loaded with either primary osteoblasts or BMP-2. A microsurgically created A-V loop was placed around the scaffold, encased in an isolation chamber in Lewis rats. HA-hydrogel supported growth of osteoblasts over 8 weeks and allowed sustained release of BMP-2 over 35 days. The A-V loop provided an angiogenic stimulus with the formation of vascularized tissue in the scaffolds. Bone-specific genes were detected by real time RT-PCR after 8 weeks. However, no significant amount of bone was observed histologically. The heterotopic isolation chamber in combination with absent biomechanical stimulation might explain the insufficient bone formation despite adequate expression of bone-related genes. Optimization of the interplay of osteogenic cells and osteo-inductive factors might eventually generate sufficient amounts of axially vascularized bone grafts for reconstructive surgery.

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Expression of hyaluronan synthases and hyaluronidases in the MG63 osteoblast cell line

Cited by 14 publications

References 54 publications

Effect of hyaluronan on osteogenic differentiation of porcine bone marrow stromal cells in vitro

Effect of hyaluronan on osteogenic differentiation of porcine bone marrow stromal cells in vitro

Hyaluronan synthesis and degradation in cartilage and bone

Hyaluronan-based heparin-incorporated hydrogels for generation of axially vascularized bioartificial bone tissues: in vitro and in vivo evaluation in a PLDLLA–TCP–PCL-composite system

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