Expression of<i>cry3A</i>gene and its toxicity against Asian Gray Weevil<i>Myllocerus undecimpustulatus undatus</i>Marshall (Coleoptera: Curculionidae)

Swamy, H. M. Mahadeva; Asokan, R.; Thimmegowda, Geetha G.; Mahmood, Riaz

doi:10.1002/jobm.201200272

Cited by 2 publications

(2 citation statements)

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“…israelensis) which stems from a Bt strain used against Dipteran species. [17][18][19][20] To our knowledge, no tests of Bt toxicity specifically against Hylobius spp. have been previously conducted.…”

Section: Introductionmentioning

confidence: 99%

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A major forest insect pest, the pine weevil Hylobius abietis, is more susceptible to Diptera‐ than Coleoptera‐targeted Bacillus thuringiensis strains

Tudoran

Nordlander

Karlberg

et al. 2020

Pest Management Science

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BACKGROUND The pine weevil (Hylobius abietis) is a major forest regeneration pest causing high levels of seedling mortality and economic losses. Current management relies on silviculture, stem coatings and insecticides. Here we evaluated for the first time the effects of Bacillus thuringiensis (Bt) strains on H. abietis adults: two producing the Coleoptera‐targeted toxins Cry3Aa (Bt tenebrionis NB‐176) and Cry8Da (Bt galleriae SDS‐502), and one producing the Diptera‐targeted Cry10A (Bt israelensis AM65‐52). Choice and nonchoice assays using individual and mixtures of Bt formulations, containing these strains respectively, were conducted. RESULTS We found that Bt had toxic and lethal effects on H. abietis, but effects varied with strain and formulation concentration. The Diptera‐targeted Bt israelensis had the most negative effects on weevil weight, feeding and mortality (70–82% feeding reduction, 65–82% greater mortality than control), whereas the effect was lower for the Coleoptera‐specific Bt tenebrionis (38–42%; 37–42%) and Bt galleriae (11–30%; 15–32%). Reduced weevil feeding was observed after 3 days, and the highest mortality occurred 7–14 days following Bt exposure. However, we found no synergistic toxic effects, and no formulation combination was better than Bt israelensis alone at reducing consumption and survival. Also, pine weevils were not deterred by Bt, feeding equally on Bt‐treated and non‐Bt treated food. CONCLUSION There is potential to develop forest pest management measures against H. abietis that include Bt, but only the Diptera‐targeted Bt israelensis would provide effective seedling protection. Its Diptera‐specificity may need reconsideration, and evaluation of other Bt strains/toxins against H. abietis would be of interest. © 2020 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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Section: Introductionmentioning

confidence: 99%

“…More recently, however, there have been reports of certain Curculionidae species being susceptible to Cry10A ( Bt ssp. israelensis ) which stems from a Bt strain used against Dipteran species 17–20 . To our knowledge, no tests of Bt toxicity specifically against Hylobius spp.…”

Section: Introductionmentioning

confidence: 99%

A major forest insect pest, the pine weevil Hylobius abietis, is more susceptible to Diptera‐ than Coleoptera‐targeted Bacillus thuringiensis strains

Tudoran

Nordlander

Karlberg

et al. 2020

Pest Management Science

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Insecticidal Activity of Bacillus thuringiensis Proteins against Coleopteran Pests

Domínguez-Arrizabalaga

Villanueva

Escriche

et al. 2020

Toxins

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Bacillus thuringiensis is the most successful microbial insecticide agent and its proteins have been studied for many years due to its toxicity against insects mainly belonging to the orders Lepidoptera, Diptera and Coleoptera, which are pests of agro-forestry and medical-veterinary interest. However, studies on the interactions between this bacterium and the insect species classified in the order Coleoptera are more limited when compared to other insect orders. To date, 45 Cry proteins, 2 Cyt proteins, 11 Vip proteins, and 2 Sip proteins have been reported with activity against coleopteran species. A number of these proteins have been successfully used in some insecticidal formulations and in the construction of transgenic crops to provide protection against main beetle pests. In this review, we provide an update on the activity of Bt toxins against coleopteran insects, as well as specific information about the structure and mode of action of coleopteran Bt proteins.

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Expression ofcry3Agene and its toxicity against Asian Gray WeevilMyllocerus undecimpustulatus undatusMarshall (Coleoptera: Curculionidae)

Cited by 2 publications

References 32 publications

A major forest insect pest, the pine weevil Hylobius abietis, is more susceptible to Diptera‐ than Coleoptera‐targeted Bacillus thuringiensis strains

A major forest insect pest, the pine weevil Hylobius abietis, is more susceptible to Diptera‐ than Coleoptera‐targeted Bacillus thuringiensis strains

Insecticidal Activity of Bacillus thuringiensis Proteins against Coleopteran Pests

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