Expression of <i>Paracoccidioides brasiliensis CHS3</i> in a <i>Saccharomyces cerevisiae chs3</i> null mutant demonstrates its functionality as a chitin synthase gene

Barreto, Laura; Sorais, Françoise; Salazar, Víctor; San-Blas, Gioconda; Niño-Vega, Gustavo

doi:10.1002/yea.1748

Cited by 16 publications

(10 citation statements)

References 23 publications

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“…The chitin synthase encoded by chsE shows upregulated expression in P. marneffei yeast cells compared to hyphae. The ortholog in P. brasiliensis also has higher expression in yeast cells compared to hyphae, and this correlates with an increase in chitin production (Barreto et al 2009). …”

Section: Resultsmentioning

confidence: 99%

Cell-Type–Specific Transcriptional Profiles of the Dimorphic Pathogen Penicillium marneffei Reflect Distinct Reproductive, Morphological, and Environmental Demands

Pasricha

Payne

Cánovas

et al. 2013

G3 Genes|Genomes|Genetics

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Penicillium marneffei is an opportunistic human pathogen endemic to Southeast Asia. At 25° P. marneffei grows in a filamentous hyphal form and can undergo asexual development (conidiation) to produce spores (conidia), the infectious agent. At 37° P. marneffei grows in the pathogenic yeast cell form that replicates by fission. Switching between these growth forms, known as dimorphic switching, is dependent on temperature. To understand the process of dimorphic switching and the physiological capacity of the different cell types, two microarray-based profiling experiments covering approximately 42% of the genome were performed. The first experiment compared cells from the hyphal, yeast, and conidiation phases to identify “phase or cell-state–specific” gene expression. The second experiment examined gene expression during the dimorphic switch from one morphological state to another. The data identified a variety of differentially expressed genes that have been organized into metabolic clusters based on predicted function and expression patterns. In particular, C-14 sterol reductase–encoding gene ergM of the ergosterol biosynthesis pathway showed high-level expression throughout yeast morphogenesis compared to hyphal. Deletion of ergM resulted in severe growth defects with increased sensitivity to azole-type antifungal agents but not amphotericin B. The data defined gene classes based on spatio-temporal expression such as those expressed early in the dimorphic switch but not in the terminal cell types and those expressed late. Such classifications have been helpful in linking a given gene of interest to its expression pattern throughout the P. marneffei dimorphic life cycle and its likely role in pathogenicity.

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Section: Resultsmentioning

confidence: 99%

Cell-Type–Specific Transcriptional Profiles of the Dimorphic Pathogen Penicillium marneffei Reflect Distinct Reproductive, Morphological, and Environmental Demands

Pasricha

Payne

Cánovas

et al. 2013

G3 Genes|Genomes|Genetics

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“…Genome analysis revealed the existence of seven chitin synthases in Paracoccidioides , one of each class, including a functional class III (Barreto et al, 2010), not described previously (Niño-Vega et al, 2000), and Pbrchs6 originally detected in transcriptome studies only in mycelia (Tomazett et al, 2005). Microarray hybridization analysis identified some chitin synthases up-regulated during the mycelium-to-yeast transition, while chitinases and endochitinases were downregulated (Nunes et al, 2005).…”

mentioning

confidence: 87%

The Paracoccidioides Cell Wall: Past and Present Layers Toward Understanding Interaction with the Host

Puccia¹,

Vallejo²,

Matsuo³

et al. 2011

Front. Microbio.

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The cell wall of pathogenic fungi plays import roles in the interaction with the host, so that its composition and structure may determine the course of infection. Here we present an overview of the current and past knowledge on the cell wall constituents of Paracoccidioides brasiliensis and P. lutzii. These are temperature-dependent dimorphic fungi that cause paracoccidioidomycosis, a systemic granulomatous, and debilitating disease. Focus is given on cell wall carbohydrate and protein contents, their immune-stimulatory features, adhesion properties, drug target characteristics, and morphological phase specificity. We offer a journey toward the future understanding of the dynamic nature of the cell wall and of the changes that may occur when the fungus infects the human host.

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“…It is therefore expected that CSIII function would depend on very precise interactions of Chs3p at the ER, such interactions not necessarily being evolutionarily conserved between S. cerevisiae and C. albicans. During the preparation of this manuscript, the functional expression of the Paracoccidiodes brasiliensis CHS3 in S. cerevisiae has been reported (Barreto et al, 2010). Unfortunately, the results reported do not confirm the functional complementation of the Scchs3D mutant.…”

Section: Structural Differences Between Cs Classes IV and V Determinementioning

confidence: 81%

Amino acid divergence between the CHS domain contributes to the different intracellular behaviour of Family II fungal chitin synthases in Saccharomyces cerevisiae

Jiménez

Sacristán

Roncero

et al. 2010

Fungal Genetics and Biology

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Expression of Paracoccidioides brasiliensis CHS3 in a Saccharomyces cerevisiae chs3 null mutant demonstrates its functionality as a chitin synthase gene

Abstract: Abstract

Cited by 16 publications

References 23 publications

Cell-Type–Specific Transcriptional Profiles of the Dimorphic Pathogen Penicillium marneffei Reflect Distinct Reproductive, Morphological, and Environmental Demands

Cell-Type–Specific Transcriptional Profiles of the Dimorphic Pathogen Penicillium marneffei Reflect Distinct Reproductive, Morphological, and Environmental Demands

The Paracoccidioides Cell Wall: Past and Present Layers Toward Understanding Interaction with the Host

Amino acid divergence between the CHS domain contributes to the different intracellular behaviour of Family II fungal chitin synthases in Saccharomyces cerevisiae

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