Expression of Ia antigens on the surface of human oligodendrocytes and astrocytes in culture

Kim, Seung Up; Moretto, Giuseppe; Shin, Doo H.

doi:10.1016/0165-5728(85)90004-9

Cited by 44 publications

(24 citation statements)

References 21 publications

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“…In vivo studies (not shown) demonstrated that, in fetal human brain sections, only a small percentage of astrocytes were spontaneously positive for HLA-DR. These findings are in agreement with several other authors who also observed a low percentage of HLA-DR positive astrocytes in vivo (Natali et al, 1981;Hauser et al, 1983;Gerosa et al, 1984), but a high percentage in culture (Kim et al, 1985;Pulver et al, 1987;Mauerhoff et al, 1988).…”

Section: Macrophage Markerssupporting

confidence: 95%

Immunocompetent cell markers in human fetal astrocytes and neurons in culture

Ennas

Cocchia

Silvetti

et al. 1992

J of Neuroscience Research

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During the past few years, evidence has accumulated that interaction with peripheral immune cells as well as immunoregulatory functions in the central nervous system (CNS) can be played by several types of brain resident cells. Since very little information is available in man, however, we investigated the presence of markers so far considered typical of immunocompetent cells in in vitro cultures of human fetal brain. Immunocytochemistry at the light, scanning, and transmission electron microscopic levels revealed positivity for a very restricted range of macrophage antigens in astrocytes, which, however, were incapable of phagocytosis. In particular, expression of the major histocompatibility complex-class II antigen HLA-DR was observed in the cytoplasm and on the cell surface of GFA-P+ astrocytes and increased with time in culture and cell passages. Among the T-lymphocyte markers tested, Thy.1 and CD4 were positive. Both neurons and astrocytes carried Thy.1 from early cell passages. Noteworthy was the presence of CD4, which serves as the receptor for AIDS virus, in neurons from the first 2 weeks, whereas astrocytes became positive after only 4-6 weeks. Even if most staining was in the cytoplasm, some was exposed on cell surface. Astrocytes were found positive for the B-lymphocyte marker CD21, the cellular receptor for Epstein-Barr virus, whereas CD24 was detected in both neurons and astrocytes. Both antigens are related to B-cell proliferation. Results are in favour of the hypothesis of human brain cells being actively involved in CNS immunological events.

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Section: Macrophage Markerssupporting

confidence: 95%

Immunocompetent cell markers in human fetal astrocytes and neurons in culture

Ennas

Cocchia

Silvetti

et al. 1992

J of Neuroscience Research

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“…A major difficulty with this hypothesis has been the inability to convincingly demonstrate MHC class II expression on oligodendrocytes, although one study (53) does report expression of MHC class II antigens on a minority of cultured human oligodendrocytes. We have now shown that in the presence of glucocorticoids, rat oligodendrocytes can readily be induced to express MHC class II mRNA and antigens by exposure to IFN-y, a factor known to be present in multiple sclerosis lesions (5).…”

Section: Discussionmentioning

confidence: 99%

In the presence of dexamethasone, gamma interferon induces rat oligodendrocytes to express major histocompatibility complex class II molecules.

Bergsteindottir

Brennan

Jessen

et al. 1992

Proc. Natl. Acad. Sci. U.S.A.

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Cells that express major histocompatibility complex (MHC) class H molecules can interact directly with CD4 T lymphocytes and either activate immune actions or become the targets of T-cell-mediated cytotoxic attack. Using rat optic nerve cultures combined with immunocytochemistry and in situ hybridization, we have shown that oligodendrocytes, the major myelin-forming cells of the central nervous system and the main casualty of the immune attacks asated with multiple sclerosis and experimental allergic encephalomyelitis, can be readily induced to express MHIC class II mRNA and surface antigens in vito by exposure to y interferon, provided the glucocorticoid dexamethasone is included in the culture medium. Oligodendrocytes exposed to v interferon without dexamethasone fail to express MHC class H molecules, which may account for the failure of previous attempts to induce expression in these cells. In the central nervous system (CNS), the major conditions of this type are multiple sclerosis and experimental allergic encephalomyelitis (EAE). In the normal CNS, cells express MHC class II molecules at low levels, if at all (4). During immunological assault, such as in multiple sclerosis or EAE, MHC class II expression is seen in association with invading monocytes/macrophages and on microglia, which are endogenous to the brain parenchyma but originate from the monocyte/macrophage lineage (4-6). Neurons themselves are considered unable to express MHC class II molecules, and the only macroglial cells implicated in antigen presentation under these circumstances are astrocytes (5-9). These cells and perinatal 0-2A progenitor cells can also be induced to express MHC class II molecules in response to y interferon (IFN-y) in vitro (4,7,(10)(11)(12). In the peripheral nervous system, IFN-y also induces MHC class II expression on Schwann cells, the glial cells of peripheral nerves (13,14). This effect may be of particular relevance in immune-related diseases, such as leprosy (15) Recombinant rat IFN-y and mouse anti-rat IFN-'y antibody (DB1) were provided by P. Van der Meide (24)

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“…More recently, however, several studies have shown that some cells of the nervous system express class I and class 11 molecules (Hauser et al, 1983;Hirsh et al, 1983;De Tribolet et al, 1984;Cadoni et al, 1986). Our own experience shows that both class I and class I1 antigens can be detected on adult human oligodendrocytes and astrocytes in culture (Kim, 1985;Kim et al, 1985b). In the present experiment, HLA-A,B,C was localized to the surface of over 80% of Schwann cells from dl four fetuses, while HLA-DR was found on two fetuses (about 50% of Schwann cells) and was absent in the other two.…”

Section: Discussionmentioning

confidence: 47%

Human fetal schwann cells in culture: Phenotypic expressions and proliferative capabibility

Kim

Yong

Watabe

et al. 1989

J of Neuroscience Research

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In this report we examined the phenotypic expressions and the proliferative capability of cultured human fetal Schwann cells. Antigens that were expressed included laminin, nerve growth factor receptor, neural cell adhesion molecule, S-100 protein, and that recognized by the monoclonal antibody HNK-1. In addition, HLA-A,B,C and HLA-DR, respectively, class I and class II antigens of the major histocompatibility complex, were demonstrated on Schwann cells. Mitotic capability was high, with an average of 34% of Schwann cells undergoing proliferation over a 2-day period.

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Expression of Ia antigens on the surface of human oligodendrocytes and astrocytes in culture

Cited by 44 publications

References 21 publications

Immunocompetent cell markers in human fetal astrocytes and neurons in culture

Immunocompetent cell markers in human fetal astrocytes and neurons in culture

In the presence of dexamethasone, gamma interferon induces rat oligodendrocytes to express major histocompatibility complex class II molecules.

Human fetal schwann cells in culture: Phenotypic expressions and proliferative capabibility

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