Expression of Il-17 in Human Memory Cd45ro+t Lymphocytes and Its Regulation by Protein Kinase a Pathway

Shin, Hyun Chul K.; Benbernou, N.; Esnault, Stéphane; Guenounou, Moncef

doi:10.1006/cyto.1998.0433

Cited by 138 publications

(101 citation statements)

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“…We report that LPMC isolated from the human gastric mucosa express IL-17 at both RNA and protein levels. This appears to be consistent with the demonstration that the vast majority of lymphocytes infiltrating the gastrointestinal mucosa bear the phenotype of Ag-experienced memory T cells (20,(23)(24)(25)(26)(27)(28)(29)(30). When RNA transcripts for IL-17 were analyzed by a semiquantitative RT-PCR, an increased accumulation was found in both mucosal tissue and LPMC samples from Hp-infected patients compared with that in uninfected subjects.…”

Section: Discussionsupporting

confidence: 88%

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Up-Regulation of IL-17 Is Associated with Bioactive IL-8 Expression inHelicobacter pylori-Infected Human Gastric Mucosa

Luzza

Parrello

Monteleone

et al. 2000

The Journal of Immunology

246

192

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Helicobacter pylori (Hp)-associated gastritis is characterized by an increased number of acute and chronic inflammatory cells secreting cytokines that contribute to maintain and expand the local inflammation. Locally induced IL-8 is believed to play a major role in the Hp-associated acute inflammatory response. Factors/mechanisms that regulate IL-8 induction are, however, not fully understood. In the present study we investigated whether Hp infection is associated with an increased production of IL-17, a T cell-derived cytokine capable of modulating IL-8 gene expression. We showed that both IL-17 RNA transcripts and protein were expressed at a higher level in the whole gastric mucosal and lamina propria mononuclear cell samples from Hp-infected patients than in those from uninfected subjects. Hp eradication was associated with a marked down-regulation of IL-17 expression. The addition of a neutralizing anti-IL-17 Ab to the gastric lamina propria mononuclear cell cultures resulted in a significant inhibition of IL-8 secretion, indicating that IL-17 contributes to enhance IL-8 in the Hp-colonized gastric mucosa. Consistently, stimulation of MKN 28 cells, a gastric epithelial cell line, with IL-17 increased IL-8 secretion. Finally, conditioned medium from the IL-17-stimulated MKN 28 cell cultures promoted the in vitro polymorphonuclear leukocyte migration. This effect was inhibitable by a neutralizing IL-8 but not IL-17 Ab. Together, these data indicate that biologically active IL-17 production is increased during Hp infection, suggesting the possibility that this cytokine may play an important role in the inflammatory response to the Hp colonization.

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Section: Discussionsupporting

confidence: 88%

“…Of the large number of cell types analyzed for IL-17 expression, it is the T lymphocyte that appears to be the only producer of IL-17 (10,11). In particular, IL-17 is produced by CD45RO ϩ , but not CD45RA ϩ , T cells (27). We report that LPMC isolated from the human gastric mucosa express IL-17 at both RNA and protein levels.…”

Section: Discussionmentioning

confidence: 75%

Up-Regulation of IL-17 Is Associated with Bioactive IL-8 Expression inHelicobacter pylori-Infected Human Gastric Mucosa

Luzza

Parrello

Monteleone

et al. 2000

The Journal of Immunology

246

192

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“…It was described initially as a product of activated and memory CD4 + T cells [23,30,30,31] but it is now known that the production of IL-17A is more ubiquitous and has been demonstrated in gd T cells [32], CD8 + memory T cells [31,33], eosinophils [34], neutrophils [31] and monocytes [35]. Nevertheless, the predominant source of IL-17A (henceforth referred to as IL-17) remains the CD4 + memory T cell population [4,33].…”

Section: Il-17mentioning

confidence: 99%

The role of T helper 17 (Th17) and regulatory T cells (Treg) in human organ transplantation and autoimmune disease

Afzali

Lombardi

Lechler

et al. 2007

Clinical and Experimental Immunology

655

470

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“…However, human memory CD8 1 T cells have been shown to secrete IL-17 in vitro following stimulation of PBMC with PMA and ionomycin [24] and murine CD8 1 T cells produce IL-17 in response to or conditioned medium from DC activated with Klebseilla pneumoniae [26].…”

Section: T-cell Subtypes That Secrete Il-17mentioning

confidence: 99%

Induction, function and regulation of IL‐17‐producing T cells

2008

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Recent reports have provided convincing evidence that IL-17-producing T cells play a key role in the pathogenesis of organ-specific autoimmune diseases, a function previously attributed exclusively to IFN-c-secreting Th1 cells. Furthermore, it appears that IL-17-producing T cells can also function with Th1 cells to mediate protective immunity to pathogens. Although much of the focus has been on IL-17-secreting CD4 1 T cells, termed The main function of IL-17-secreting T cells is to mediate inflammation, by stimulating production of inflammatory cytokines, such as TNF-a, IL-1b and IL-6, and inflammatory chemokines that promote the recruitment of neutrophils and macrophages.Key words: Autoimmunity . IL-17 . Infection . Inflammation IntroductionMore than 20 years ago Mosmann and Coffman reported that distinct CD4 1 T-cell subtypes, termed Th1 and Th2 cells, could be distinguished on the basis of cytokine secretion and function [1]. The Th1-Th2 model provided a useful but simplistic basis for our understanding of the mechanisms of immunity to infection and also attempted to explain the role of T cells in the pathogenesis of autoimmunity and allergy/asthma. The original hypothesis was that IFN-g-secreting CD4 1 cells mediated protective immunity to intracellular pathogens by promoting macrophage activation and stimulating production of complement fixing and virus neutralizing antibodies, but also promoted inflammatory responses and mediated the pathology in certain autoimmune diseases. In contrast, CD4 1 T cells that secreted IL-4, IL-5, IL-10 and IL-13 were considered to be the main helper T cells providing help for B-cell production of antibody, especially IgG1 in mice, and mediated protective immunity to extracellular pathogens. These Th2 cells were also anti-inflammatory, controlling Th1 responses, but at the same time mediated allergic reactions and had an inflammatory and pathological role in asthma.The identification of additional T-cell subtypes has led to a shift in the Th1-Th2 paradigm and has helped to explain some anomalies in the original model. In the mid 1990s suppressor T cells were re-discovered as Treg cells and were shown to have a major role in controlling immune responses to self-antigens, thereby preventing autoimmunity. Furthermore, these cells rather than the reciprocal Th1 or Th2 populations were shown to be the major regulator of effector T cells, functioning to maintain self-tolerance, prevent autoimmunity and limit collateral damage during immune responses to pathogens [2,3].There were a number of other observations which could not be explained on the basis of the two Th1 and Th2 subtypes. Mini-ReviewDespite the assumption that Th1 cells mediated autoimmunity, mice deficient in IFN-g or IFN-g receptors were found to have increased susceptibility to EAE and collagen-induced arthritis (CIA) [4,5]. Furthermore, EAE was exacerbated in mice deficient in the Th1 polarizing cytokine, 7]. It was then discovered that mice deficient in the novel IL-12 family member, IL-23 were resistant to EAE [6]....

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Expression of Il-17 in Human Memory Cd45ro+t Lymphocytes and Its Regulation by Protein Kinase a Pathway

Cited by 138 publications

References 0 publications

Up-Regulation of IL-17 Is Associated with Bioactive IL-8 Expression inHelicobacter pylori-Infected Human Gastric Mucosa

Up-Regulation of IL-17 Is Associated with Bioactive IL-8 Expression inHelicobacter pylori-Infected Human Gastric Mucosa

The role of T helper 17 (Th17) and regulatory T cells (Treg) in human organ transplantation and autoimmune disease

Induction, function and regulation of IL‐17‐producing T cells

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