Expression of IL-1α, IL-6, TGF-β, FasL and ZNF265 During Sertoli Cell Infection by Ureaplasma Urealyticum

Rongping, Li; Yang, Xi; Liu, Xiuzhi; Chen, Guangjie; Wang, Baoguo; Li, Jiang; Li, Weiyi

doi:10.1038/cmi.2009.29

Cited by 11 publications

(7 citation statements)

References 25 publications

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“…TGF- β is capable of recognizing itself adequately, foreign or tumor-associated antigens, and inhibits the production and/or effects of proinflammatory cytokines (such as IL-1 α , IL-6, and TNF- α ) [ 29 , 30 ]. Li et al [ 31 ] indicate that expression levels of IL-1 α , IL-6, and TGF- β are provoked in the second week after the urinary bladder is injected with UU.…”

Section: Discussionmentioning

confidence: 99%

Zhibai Dihuang Pill Alleviates Ureaplasma urealyticum-Induced Spermatogenic Failure and Testicular Dysfunction via MAPK Signaling Pathway

Zhang

Guo²,

Zhu³

et al. 2022

Computational and Mathematical Methods in Medicine

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The testicles and sperm are extremely susceptible to inflammation and oxidative stress. Although Zhibai Dihuang Pill (ZDP) has been reported to treat various infertilities including male infertility induced by Ureaplasma urealyticum (UU) infection, its mechanism is still poorly understood. This study is aimed at clarifying the underlying mechanism of ZDP to protect against UU-infected male infertility. We found that UU-infected infertile rats exhibited weight loss, reduced food intake, and decreased sperm count and vitality. The administration of ZDP improved the general state and sperm motility of rats. In addition, UU infection led to spermatogenesis disorders, impaired secretory function and blood-testis barrier (BTB) of Sertoli cells, and elevated inflammation and oxidative stress. As expected, ZDP suppressed inflammation and oxidative stress to alleviate spermatogenesis disorders. Our research showed that ZDP could improve spermatogenesis disorders and testicular function primarily through the mitogen-activated protein kinase (MAPK) signaling pathway. ZDP exerts its anti-inflammatory and antioxidant effects via the MAPK signaling pathway, thus playing an important role in ameliorating spermatogenesis failure and testicular dysfunction.

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Section: Discussionmentioning

confidence: 99%

Zhibai Dihuang Pill Alleviates Ureaplasma urealyticum-Induced Spermatogenic Failure and Testicular Dysfunction via MAPK Signaling Pathway

Zhang

Guo²,

Zhu³

et al. 2022

Computational and Mathematical Methods in Medicine

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“…IL-6 production under physiological conditions is lowest at stages VII-VIII of the seminiferous epithelium cycle 18 . However, testicular IL-6 levels are up-regulated in the setting of injury and inflammation 25 32 33 34 35 11 . Herein, IL-6 could affect the contractility of BTB by changing the localization and amount of BTB-constituent proteins, while the mRNA levels of BTB-constituent proteins were not changed.…”

Section: Discussionmentioning

confidence: 99%

“…In the testis, IL-6 inhibits meiotic DNA synthesis during the cycle of the seminiferous epithelium 18 , influences the secretion of transferrin and inhibin B by Sertoli cells 19 20 and reduces sperm motility 21 . During testicular infection and inflammation in response to agents such as lipopolysaccharide or Ureaplasma urealyticum , the level of IL-6 expression increases 22 23 24 25 . In this study, the roles of IL-6 in the regulation of the Sertoli cell barrier were investigated.…”

mentioning

confidence: 99%

Interleukin-6 disrupts blood-testis barrier through inhibiting protein degradation or activating phosphorylated ERK in Sertoli cells

Zhang

Yin

Wang

et al. 2014

Sci Rep

116

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It has been recently ascribed to several inflammatory cytokines (i.e. TGF-β3, TNF-α, and IL-1) a functional role in regulating Sertoli cell blood-testis barrier (BTB) dynamics. In the testis, IL-6 inhibits meiotic DNA synthesis during the seminiferous epithelium cycle, reduces sperm motility and influences the secretion of transferrin and inhibin B by Sertoli cells. Also, it has been shown that IL-6 affects tight junction permeability in Sertoli cells, but, little is known about its role in regulating the BTB. The aim of this study was to investigate the molecular mechanisms by which IL-6 affects BTB dynamics. We show that IL-6 perturbs the integrity of the BTB, and alters the normal localization and steady-state levels of BTB integral membrane proteins. We demonstrated that IL-6 regulates the BTB by inhibiting the degradation of BTB constitutive proteins and activating ERK-MAPK pathways. Our results provide mechanistic insight into the roles of IL-6 in regulating BTB dynamics.

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“…IL-6 is expected to cooperate with IL-1alpha to strengthen the local inflammatory reaction directly or through affecting some immune cells response in testis during early infection. (11) .IL-6 is a 26 Dka protein produced by a number of cell types, including Sertoli cells (12). Several evidences indicates that cytokines are involved in male infertility; they are secreted by different parts of the male genetic tract and may exert effects on the steroidogenesis, spermatogenesis and sperm function (13,14) .In response to inflammation of the testis, Sertoli cells play their regulatory role via secretion of a series of cytokines (15).…”

Section: Effect Of Body Mass Index On Interleukin2 6 and Soluble Fibr...mentioning

confidence: 99%

Effect of Body mass index on interleukin2, 6 and soluble fibroblast associated surface antigen in infertile men

Jaafar¹,

Ahmed²,

Alsalihi³

2015

JFacMedBagdad

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Background: obesity is an important cause of adverse health problems, including male infertility. Testosterone is essential for spermatogenesis and permits the release of mature sperms. In the absence of testosterone stimulation, spermatogenesis does not proceed beyond the meiosis stage. Aromatase expression is directly related to the degree of adiposity; it is dependent on cytokine stimulation and requires the presence of glucocorticoids, 17β-estradiol in the plasma of adult men is formed by aromatization convert testosterone and androstinedione to 17β-estradiol Apoptosis is an important process in the context of germ cells since they undergo both mitosis and meiosis, and this process is affected by interleukin 6and 2 (IL6 and IL2).Objectives: To assess the effect of body mass index on serum sex steroidal hormones, seminal antiapoptotic factor soluble fibroblast associated surface antigen (sFas) and inflammatory markers (IL6, IL-2), with conventional semen parameters in infertile men.Patients and methods: One hundred and six male partners of infertile couples were involved in the study. Height and weight of them were measured to calculate the body mass index (BMI). Serum testosterone and estradiol 2.were measured for all.Semen sample was taken after 2-7 days of abstinence. Conventional semen analysis was done for each sample according to the protocol of (WHO) 2010, after incubation and liquefaction period (30-60 min). Semen plasma was collected for analysis of interleukins (2 and 6) and sFAS .by specific kits Patients with severe oligospermia (below million sperm/ml) were excluded from the study. The rest (77) subjects were divided into three groups according to BMI (WHO, 2010) as follows: Normal (BMI < 25 kg/ m2) (n=24): Overweight (BMI ≥ 25 and < 30 kg/m2) (n=30): Obese (BMI > 30 kg/m2) (n=23).Results: The results showed a significant decrease in Testosterone level and Testosterone/E2 ratio in overweigh and obese subjects as compared to normal. The results of conventional semen analysis showed significant difference in pH and no. of round cell between the two groups. There was positive correlation between IL2 with sFAS in the two groups, and negative correlation of IL2 with total progressive cell and motility in both overweigh and obese groups. There was positive correlation of IL6 with no. of round cells in both studied groups.Conclusion: BMI affects Testosterone level and Testosterone/E2 ratio (they were significantly decreased in overweigh and obese patients). A positive correlation between IL2 and sFAS in obese patients as well as positive correlation of IL6 with round cells in obese patients indicate the role of apoptosis which is essential for normal fertility to remove abnormal and unwanted cells in ejaculate, in infertile overweight and obese patients.

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Expression of IL-1α, IL-6, TGF-β, FasL and ZNF265 During Sertoli Cell Infection by Ureaplasma Urealyticum

Abstract: To investigate immunoregulatory mechanisms of Sertoli cells in the testis

Cited by 11 publications

References 25 publications

Zhibai Dihuang Pill Alleviates Ureaplasma urealyticum-Induced Spermatogenic Failure and Testicular Dysfunction via MAPK Signaling Pathway

Zhibai Dihuang Pill Alleviates Ureaplasma urealyticum-Induced Spermatogenic Failure and Testicular Dysfunction via MAPK Signaling Pathway

Interleukin-6 disrupts blood-testis barrier through inhibiting protein degradation or activating phosphorylated ERK in Sertoli cells

Effect of Body mass index on interleukin2, 6 and soluble fibroblast associated surface antigen in infertile men

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