Expression of immune genes in Indian major carp, Catla catla challenged with Flavobacterium columnare

Ravindra,; Pradhan, Pravata Kumar; Paria, Anutosh; Pande, Veena; Verma, Dev Kumar; Arya, Prabhat; Rathore, Gaurav; Sood, Neeraj

doi:10.1016/j.fsi.2019.09.052

Cited by 14 publications

(6 citation statements)

References 60 publications

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“…In the present study, the upregulation of TGF-β was most notable in the head kidney, spleen, and hindgut—particularly in the fish from the BI vaccine group after infection with S. iniae . These findings are consistent with the previous studies on TGF-β expression in systemic and mucosal organs infected by bacteria and parasites [ 40 , 41 ]. Furthermore, in this study, the TGF-β increased in post-challenge fish, suggesting that BI-vaccinated fish maintain the dynamic balance between the inflammatory reactions and avoid triggering needless damage to the host [ 28 ].…”

Section: Discussionsupporting

confidence: 94%

Effect of an Oral Bivalent Vaccine on Immune Response and Immune Gene Profiling in Vaccinated Red Tilapia (Oreochromis spp.) during Infections with Streptococcus iniae and Aeromonas hydrophila

Monir

Yusoff

Zamri‐Saad

et al. 2022

Biology

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Streptococcosis and aeromonasis inflicted by Streptococcus iniae and Aeromonas hydrophila, respectively, have affected tilapia industries worldwide. In this study, we investigated antibody responses and explored the mechanisms of protection rendered by an oral bivalent vaccine in red tilapia following challenges with S. iniae and A. hydrophila. The results of specific IgM antibody response revealed that the IgM titers against S. iniae and A. hydrophila in the bivalent incorporated (BI) vaccine group were significantly higher (p < 0.05) than those in the bivalent spray (BS) vaccine fish and unvaccinated control fish throughout the experiment. Real-time qPCR results also showed that the gene expression of CD4, MHC-I, MHC-II, IgT, C-type lysozyme, IL-1β, TNF-α, and TGF-β remained significantly higher (p < 0.05) than that of the controls between 24 and 72 h post-infection (hpi) in both mucosal (hindgut) and systemic (spleen and head–kidney) organs of BI vaccinated fish. Furthermore, the highest relative expression of the TGF-β, C-type lysozyme, and IgT genes in the BI vaccinated group was observed in the challenged fish’s spleen (8.8-fold), head kidney (4.4-fold), and hindgut (19.7-fold) tissues, respectively. The present study suggests that the bivalent incorporated (BI) vaccine could effectively improve the immune function and activate both humoral and cell-mediated immunities in vaccinated red tilapia following the bacterial challenges.

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Section: Discussionsupporting

confidence: 94%

Effect of an Oral Bivalent Vaccine on Immune Response and Immune Gene Profiling in Vaccinated Red Tilapia (Oreochromis spp.) during Infections with Streptococcus iniae and Aeromonas hydrophila

Monir

Yusoff

Zamri‐Saad

et al. 2022

Biology

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“…These findings supported the vital role of SC in activating the immune responses in sea bream and improving its resistance to pathogens. Fish fed with 4 g/kg SC-supplemented diet in this study exhibited significant upregulation of the pro-inflammatory cytokine IL-1β , which has a vital role in adjusting the immune response of fish [ 85 ] similar to what have been reported by Yang et al [ 86 ] in the orange-spotted grouper. Furthermore, the 4 g/kg SC diet-fed fish showed significant downregulation of HSP70 , which is expressed in fish exposed to environmental stressors [ 87 ], pinpointing the anti-stress activity of SC.…”

Section: Discussionsupporting

confidence: 83%

The Effect of Dietary Saccharomyces cerevisiae on Growth Performance, Oxidative Status, and Immune Response of Sea Bream (Sparus aurata)

El-Bab

Saghir

El-Naser

et al. 2022

Life

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The objective of this study was to evaluate the beneficial effect of Saccharomyces cerevisiae (SC) on growth, intestinal morphometric characteristics, blood indices, redox balance, expression of immune-related genes, and their involvement in disease resistance in sea bream (Sparus aurata). Three hundred healthy sea bream fingerlings were allocated into equal four groups (15 fish per hapa). The first group was served as a control and received a basal diet, while the other three groups were fed diets containing 1, 2, and 4 g/kg diet SC, respectively. At the end of week 16, the daily weight gain, specific growth rate, and feed utilization were significantly higher in the SC2 and SC4 groups than the control (p < 0.05). SC dose-dependently improved intestinal morphology, and the 4 g/kg diet significantly increased dry matter, crude fat, and crude protein percentage of body composition when compared with the control group. The 4 g/kg SC boosted innate immune response and phagocytic activity, and all SC-supplemented diets improved total protein, glucose, triglycerides, and urea concentrations, as well as intestinal digestive enzymatic activities. All estimated oxidative markers were significantly enhanced in the group that received 4 g/kg SC when compared with the control and other SC groups (p < 0.05). Feeding the fish a diet supplemented with 4 g/kg SC markedly regulated the expression of HSP70, IGF1, and IL-1β genes. In addition, the 4 g/kg SC-supplemented diet was the most effective in protecting the fish against Vibrio parahaemolyticus challenge. In conclusion, SC-enriched diet improved growth performance, intestinal morphology, redox homeostasis, and immune response of S. aurata with the 4 g/kg concentration as the most effective.

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“…Among the cytokines detected as up-regulated earlier in WB (i.e., at 1 h or 4 h post-infection) compared to HSB (up-regulated at 24 h post-infection), several have well-characterized roles in immune response mechanisms and include: interleukin-1 beta (IL-1β), interleukin-17C-like (IL-17C), tumor necrosis factor alpha (TNF-α), granulocyte colony-stimulating factor-like (G-CSF), interleukin 8 (IL-8), C—C motif chemokine 16-like (CCL16), and C-X-C motif chemokine 9-like (CXCL9) ( Table 4 ). Pro-inflammatory IL-1β is an activator of a major immune signaling effector, nuclear factor-κB (NF-κB), and, among other functions, facilitates a prompt response to infection or injury via inflammation [33] . As such, increased expression of IL-1β earlier in the presence of a pathogen, such as that detected here, is presumably advantageous, and, moreover, the down-regulation of IL-1β in HSB at 4 h post-infection, disadvantageous.…”

Section: Discussionmentioning

confidence: 99%

Gene expression profiles of white bass (Morone chrysops) and hybrid striped bass (M. chrysops x M. saxatilis) gill tissue following Flavobacterium covae infection

Andersen,

Abernathy,

Farmer

et al. 2024

Comparative Immunology Reports

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Expression of immune genes in Indian major carp, Catla catla challenged with Flavobacterium columnare

Cited by 14 publications

References 60 publications

Effect of an Oral Bivalent Vaccine on Immune Response and Immune Gene Profiling in Vaccinated Red Tilapia (Oreochromis spp.) during Infections with Streptococcus iniae and Aeromonas hydrophila

Effect of an Oral Bivalent Vaccine on Immune Response and Immune Gene Profiling in Vaccinated Red Tilapia (Oreochromis spp.) during Infections with Streptococcus iniae and Aeromonas hydrophila

The Effect of Dietary Saccharomyces cerevisiae on Growth Performance, Oxidative Status, and Immune Response of Sea Bream (Sparus aurata)

Gene expression profiles of white bass (Morone chrysops) and hybrid striped bass (M. chrysops x M. saxatilis) gill tissue following Flavobacterium covae infection

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