Expression of Indica rice OsBADH1 gene under salinity stress in transgenic tobacco

Hasthanasombut, Supaporn; Ntui, Valentine Otang; Supaibulwatana, Kanyaratt; Mii, Masahiro; Nakamura, Ikuo

doi:10.1007/s11816-009-0123-6

Cited by 54 publications

(22 citation statements)

References 35 publications

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“…However, there was a 10-fold (0.15-0.25 lmol g -1 FW) increase in the GB levels when salt stress was imposed. Although the preliminary experiment in transgenic tobacco showed higher amounts of GB accumulation (0.14-0.56 lmol g -1 FW; Hasthanasombut et al 2010), it has been shown that the maize ubiquitin promoter induces stronger gene expression in monocots than in dicots (McElroy and Brettell 1994), therefore, this result suggests that the Indica OsBADH1 mRNA was also partially degraded in transgenic Japonica rice.…”

Section: Discussionmentioning

confidence: 87%

“…Then, the XbaI-XhoI fragment from pET29 was linked between the promoter of the maize ubiquitin 1 gene and the terminator of the nopaline synthase (nos) gene in pSub221s plasmid. Agrobacterium strain EHA101 harboring binary plasmid vector (pEKH2/ Ubi::OsBADH1) was constructed as described previously (Hasthanasombut et al 2010).…”

Section: Production Of Transgenic Osbadh1 Rice and Growth Conditionsmentioning

confidence: 99%

“…Then, RNA was treated with DNase I (Takara Bion Inc., Shiga, Japan) to remove DNA contamination. Reverse transcription PCR (RT-PCR) was performed as described previously by Hasthanasombut et al (2010).…”

Section: Analysis Of Osbadh1 Gene Expression By Rt-pcrmentioning

confidence: 99%

“…There was a significant increase in salt resistance in OsBADH1 transgenic tobacco plants under salinity-stress conditions (Hasthanasombut et al 2010). However, there have been no reports of OsBADH1 overexpression in rice plants.…”

Section: Introductionmentioning

confidence: 97%

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Genetic manipulation of Japonica rice using the OsBADH1 gene from Indica rice to improve salinity tolerance

Hasthanasombut

Supaibulwatana

Mii

et al. 2010

Plant Cell Tiss Organ Cult

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Betaine aldehyde dehydrogenase (BADH) is a major oxidative enzyme that converts betaine aldehyde to glycine betaine (GB), an osmoprotectant compound in plants. Japonica rice (salt-sensitive) was genetically engineered to enhance salt tolerance by introducing the OsBADH1 gene from Indica rice (salt-tolerant), which is a GB accumulator. We produced transgenic rice plants overexpressing the modified OsBADH1 gene under the control of the maize ubiquitin promoter. The transgenic rice showed increased OsBADH1 gene expression and OsBADH1 enzyme production, resulting in the accumulation of GB. It also exhibited enhanced salt tolerance in immature and mature transgenic rice seedlings. The adverse effect of salt stress on seed germination, the growth of immature and mature seedlings, water status, and photosynthetic pigments was alleviated in transgenic seedlings.

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Section: Discussionmentioning

confidence: 87%

Section: Production Of Transgenic Osbadh1 Rice and Growth Conditionsmentioning

confidence: 99%

Section: Analysis Of Osbadh1 Gene Expression By Rt-pcrmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

See 2 more Smart Citations

Genetic manipulation of Japonica rice using the OsBADH1 gene from Indica rice to improve salinity tolerance

Hasthanasombut

Supaibulwatana

Mii

et al. 2010

Plant Cell Tiss Organ Cult

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“…Thus, our transgenic plants possibly also accumulated GABA and gained improved salt stress tolerance, since GABA can function as an osmoprotectant in plants (Mazzucotelli et al 2006). Most recently, transgenic tobacco (Nicotiana tabacum L.) plants expressing OsBADH1 from indica rice showed improved salt stress tolerance (Hasthanasombut et al 2010). Those plants might also accumulate GABA, although no data have been presented in the literature.…”

Section: Discussionmentioning

confidence: 89%

Production of transgenic Italian ryegrass expressing the betaine aldehyde dehydrogenase gene of zoysiagrass

Takahashi

Oishi²,

Ebina

et al. 2010

Breed. Sci.

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Transgenic Italian ryegrass plants expressing the betaine aldehyde dehydrogenase gene of zoysiagrass (ZBD1) were produced via particle bombardment of embryogenic calluses. Although the growth of both non-transgenic and transgenic plants expressing ZBD1 was inhibited under salt stress, isolated tillers of the transgenic plants showed better re-rooting ability in vitro in the presence of 300 mM NaCl. Chlorophyll fluorescence values of the transgenic plants declined under salt stress, but were significantly higher than those of non-transgenic plants under salt stress, indicating the improved salt stress tolerance of transgenic plants.

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Metabolic Profiling to Elucidate Genetic Elements Due to Salt Stress

Ahmad

Jamil

Shahzad

et al. 2017

CLEAN Soil Air Water

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Soil salinity is a prevalent abiotic stress and affects around 20% of the irrigated land worldwide. Saline soil affects plant mineral nutrition and water uptake capacity. Plants adopt several strategies to combat salt stress such as synthesis of compatible solutes, control of ions uptake and transportation, production of enzymes and plant growth hormones, and ions accumulation or exclusion. Moreover, plants can also adapt to salt stress through a change in the whole metabolomics to overcome salt stress over a period of time. Metabolites, such as sugars, for instance, are important targets for mitigation of symptoms of salt stress. In spite of the scientific progress to date, still massive research work is to be done to investigate the actual physiological and molecular mechanism of metabolome and the underlying involved genes associated with salt resistance. In addition, expression, regulation, and function of genes and genetic pathways of metabolites, for example, proline, glycine betaine, abscisic acid, jasmonates, and flavonoids, that lead to the reestablishment of proper cellular ionic and osmotic homeostasis during salt stress, are not yet completely understood. This review presents an overview of past and current research on how plants, especially important crop plants, combat salt stress. Further, an attempt has been made to evaluate the gaps in current research and explore the role of genetic studies involved in metabolomics in order to enhance crop yield of salt‐affected soils in future.

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Expression of Indica rice OsBADH1 gene under salinity stress in transgenic tobacco

Cited by 54 publications

References 35 publications

Genetic manipulation of Japonica rice using the OsBADH1 gene from Indica rice to improve salinity tolerance

Genetic manipulation of Japonica rice using the OsBADH1 gene from Indica rice to improve salinity tolerance

Production of transgenic Italian ryegrass expressing the betaine aldehyde dehydrogenase gene of zoysiagrass

Metabolic Profiling to Elucidate Genetic Elements Due to Salt Stress

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