Expression of insulin-like growth factor-II (IGF-II) messenger ribonucleic acid (mRNA), but not IGF-I mRNA, in human preovulatory granulosa cells

Geisthövel, F.; Moretti-Rojas, Ines; Asch, Ricardo H.; Rojas, Francisco J.

doi:10.1093/oxfordjournals.humrep.a137007

Cited by 75 publications

(19 citation statements)

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“…IGF-I and IGF-II have been detected in FFs from women with natural men strual cycles and from patients who underwent IVF-ET [17,18], Oocytes and granulosa cells from human ovaries have been shown to contain IGF receptors [19]. It has been suggested that IGFs stimulate follicular growth, dif ferentiation and steroidogenesis.…”

Section: Discussionmentioning

confidence: 99%

Insulin-Like Growth Factor-Binding Protein-1 in Human Follicular Fluid: A Marker for Oocyte Maturation

Kawano¹,

Narahara²,

Matsui³

et al. 1997

Gynecol Obstet Invest

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In order to investigate the role of insulin-like growth factors (IGFs) in human ovulation, we evaluated the concentrations of IGF-binding proteins (IGFBPs) in human follicular fluid (FF). The concentrations of IGFBP-1 in the FFs of 15 women undergoing in vitro fertilization and embryo transfer were measured and related to those of 17Β-estradiol (E₂), progesterone and androstenedione in the FFs. IGFBP-1 levels in the FFs were positively correlated with those of E₂ and progesterone. No correlation was found between the IGFBP-1 and androstenedione levels in FFs. The concentrations of IGFBP-1 were significantly increased in the FFs which contained mature oocytes compared with those of immature oocytes, whereas IGFBP-3 in FFs tended to decrease as oocytes matured. It is suggested that IGFs may play important roles in human preovulatory processes, and that IGFBP-1 may be a valuable biochemical marker in the evaluation of oocytes.

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Section: Discussionmentioning

confidence: 99%

Insulin-Like Growth Factor-Binding Protein-1 in Human Follicular Fluid: A Marker for Oocyte Maturation

Kawano¹,

Narahara²,

Matsui³

et al. 1997

Gynecol Obstet Invest

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“…In rat and bovine GCs, IGF-I activates PI3-K and Akt, with IGF-I driving phosphorylation, indicating that IGF-I plays an anti-apoptotic role in GCs by sustaining PI3-K-Akt signaling [100]. Although IGF-I has an essential role in the development of ovarian follicles in many species, IGF-II is more abundant and likely more important in the human and non-human primate ovary, where it appears to act similarly to IGF-I [144][145][146]. All these observations led to the suggestion that the ultimate fate of the follicle depends on intrafollicular synthesis and bioavailability of IGF, and ultimately to its interaction with its cognate receptor [147].…”

Section: Gonadotropins and Intraovarian Regulatorsmentioning

confidence: 99%

Life and death of female gametes during oogenesis and folliculogenesis

et al. 2008

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The vertebrate ovary is an extremely dynamic organ in which excessive or defective follicles are rapidly and effectively eliminated early in ontogeny and thereafter continuously throughout reproductive life. More than 99% of follicles disappear, primarily due to apoptosis of granulosa cells, and only a minute fraction of the surviving follicles successfully complete the path to ovulation. The balance between signals for cell death and survival determines the destiny of the follicles. An abnormally high rate of cell death followed by atresia can negatively affect fertility and eventually lead irreversibly to premature ovarian failure. In this review we provide a short overview of the role of programmed cell death in prenatal differentiation of the primordial germ cells and in postnatal folliculogenesis. We also discuss the issue of neo-oogenesis. Next, we highlight molecules involved in regulation of granulosa cell apoptosis. We further discuss the potential use of scores for apoptosis in granulosa cells and characteristics of follicular fluid as prognostic markers for predicting the outcome of assisted reproduction. Potential therapeutic strategies for combating premature ovarian failure are also addressed.

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“…In addition, primary granulosa cells cultured in vitro undergo a spontaneous luteinization that makes it difficult to identify and characterize growth factors, cytokines, and other possible factors regulating the proliferation and differentiation of granulosa cells, or to study how cytokines interact to manifest various biological responses. Previously, the granulosa cell has been shown to be a source and target cell for growth and differentiation factors, such as members of the transforming growth factor 9 (TGF3) family of proteins [1,[9][10][11][12][13], epidermal growth factor (EGF)-like proteins [14][15][16][17], fibroblast growth factors (FGFs) [18][19][20], insulin-like growth factors (IGFs) [1,[21][22][23][24][25][26], and interleukins [27]. However, conflicting data were published concerning the exact location or subtype expressed within ovarian tissues.…”

Section: Introductionmentioning

confidence: 99%

Secretion of Steroids, Growth Factors, and Cytokines by Immortalized Mouse Granulosa Cell Lines1

Vanderstichele

Delaey

Winter

et al. 1994

Biology of Reproduction

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The expression and function of gonadotropin receptors, and the secretion of steroids, transferrin, and cytokines were investigated in three immortalized (single transfection with v-myc) mouse granulosa cell lines (GRM01, GRM01L, and GRM02). A dose-dependent increase in progesterone production was obtained in GRM01 and GRM02 cells after addition of LH, FSH, modulators of the adenylate cyclase enzyme system, and cAMP analogues. The LH-induced release of progesterone was already detectable in GRM02 cells after 8 h and was related to incubation time and cell number. Both epidermal growth factor (EGF) and transforming growth factor alpha (TGF alpha) induced the secretion of progesterone in GRM02 cells, while no effect was obtained with TGF beta. LH receptor concentration was highest in the GRM02 cell line. FSH receptor mRNA was visualized in GRM01 and GRM02 cells. Aromatase activity in GRM02 cells was induced by androgens and inhibited by aromatase inhibitors. Whereas all cell lines were able to secrete transferrin, only in GRM01 cells was transferrin secretion increased significantly by LH. FSH did not affect transferrin secretion in the three cell lines, in contrast to forskolin or 8-bromo-cAMP. The immortalized mouse granulosa cell lines were able to express and release several growth factors. The expression and secretion of activin, inhibin, TGF beta, EGF, TGF alpha, insulin-like growth factor II, fibroblast growth factor (acidic and basic), platelet-derived growth factor, and interleukin-6 suggest an autocrine or paracrine role for these factors in follicular differentiation and function. In conclusion, these cells, derived from mural granulosa cells and immortalized in a preovulatory state, can be used to study granulosa cell physiology or to study the role of granulosa cells and their derivatives in the process of follicular maturation, fertilization, and early embryonic development.

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Expression of insulin-like growth factor-II (IGF-II) messenger ribonucleic acid (mRNA), but not IGF-I mRNA, in human preovulatory granulosa cells

Cited by 75 publications

References 0 publications

Insulin-Like Growth Factor-Binding Protein-1 in Human Follicular Fluid: A Marker for Oocyte Maturation

Insulin-Like Growth Factor-Binding Protein-1 in Human Follicular Fluid: A Marker for Oocyte Maturation

Life and death of female gametes during oogenesis and folliculogenesis

Secretion of Steroids, Growth Factors, and Cytokines by Immortalized Mouse Granulosa Cell Lines1

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