Expression of Intercellular Adhesion Molecule-1 (ICAM-1) During Ischemia–Reperfusion in Human Liver Tissue Allograft: Image Analysis by Confocal Laser Scanning Microscopy

Benkoël, L; Dodero, F; Hardwigsen, Jean; Benoliel, Anne‐Marie; Bongrand, Pierre; Botta‐Fridlund, Danielle; Treut, Yves Patrice Le; Chamlian, A; Lagadic‐Gossmann, Dominique

doi:10.1023/b:ddas.0000004521.88660.fd

Cited by 13 publications

(7 citation statements)

References 36 publications

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“…However, given that these lesions are typically examined by means of FS, they are probably incorporated in other more general studies about digital pathology for intraoperative consultation. Most studies on organ assessment (14/19, 73.7%) were mainly about liver and kidney biopsy,[414151617181920212223242526] while only a small proportion (5/19, 26.3%)[2728293031] dealt with pancreatic islet preparations for transplant. With regard to the type of digital pathology technology used, 12/19 (63.2%) studies discussed DIA applied to LM-acquired images, 4/19 (21.1%) studies used WSI,[15162526] one study involved only static telepathology without DIA,[4] another referred generally to using a “virtual microscope,”[24] and one did not clarify the type of digital pathology used.…”

Section: Methodsmentioning

confidence: 99%

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The Landscape of Digital Pathology in Transplantation: From the Beginning to the Virtual E-Slide

Girolami¹,

Parwani

Barresi³

et al. 2019

Journal of Pathology Informatics

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Background: Digital pathology has progressed over the last two decades, with many clinical and nonclinical applications. Transplantation pathology is a highly specialized field in which the majority of practicing pathologists do not have sufficient expertise to handle critical needs. In this context, digital pathology has proven to be useful as it allows for timely access to expert second-opinion teleconsultation. The aim of this study was to review the experience of the application of digital pathology to the field of transplantation. Methods: Papers on this topic were retrieved using PubMed as a search engine. Inclusion criteria were the presence of transplantation setting and the use of any type of digital image with or without the use of image analysis tools; the search was restricted to English language papers published in the 25 years until December 31, 2018. Results: Literature regarding digital transplant pathology is mostly about the digital interpretation of posttransplant biopsies (75 vs. 19), with 15/75 (20%) articles focusing on agreement/reproducibility. Several papers concentrated on the correlation between biopsy features assessed by digital image analysis (DIA) and clinical outcome (45/75, 60%). Whole-slide imaging (WSI) only appeared in recent publications, starting from 2011 (13/75, 17.3%). Papers dealing with preimplantation biopsy are less numerous, the majority (13/19, 68.4%) of which focus on diagnostic agreement between digital microscopy and light microscopy (LM), with WSI technology being used in only a small quota of papers (4/19, 21.1%). Conclusions: Overall, published studies show good concordance between digital microscopy and LM modalities for diagnosis. DIA has the potential to increase diagnostic reproducibility and facilitate the identification and quantification of histological parameters. Thus, with advancing technology such as faster scanning times, better image resolution, and novel image algorithms, it is likely that WSI will eventually replace LM.

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Section: Methodsmentioning

confidence: 99%

“…examined the expression of different IHC markers in a subset of paired preimplantation and postreperfusion liver biopsies, using DIA of confocal laser scanning microscope images, without comparison to conventional LM IHC. [141920]…”

Section: Methodsmentioning

confidence: 99%

The Landscape of Digital Pathology in Transplantation: From the Beginning to the Virtual E-Slide

Girolami¹,

Parwani

Barresi³

et al. 2019

Journal of Pathology Informatics

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“…(Benkoel et al, 2003) ↑ Acute phase C-reactive protein ↑ (Watchorn et al, 2001) n/e Angiotensinogen ↑ (Li and Brasier, 1996) -? Apoptosis Bcl2 - (Imose et al, 2003) …”

Section: Genesmentioning

confidence: 99%

A mechanism for the anti-fibrogenic effects of the pregnane X receptor (PXR) in the liver: Inhibition of NF-κB?

et al. 2008

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“…ICAM-1 has been shown to be of particular importance in the adhesion of polymorphonuclear neutrophils (PMNs) to SECs and hepatocytes. After transendothelial migration, PMNs are responsible for the induction and progression of hepatic injury (7,10,12,28). We have shown that ICAM-1 expression is negligible around the central vein (zone III) in the HP group compared with the C group.…”

Section: Discussionmentioning

confidence: 86%

Downregulation of Cytokine Release by Heat Preconditioning of Livers Used for Transplantation in Rats

et al. 2005

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We evaluated the changes in the levels of released cytokines following heat preconditioning of the livers used in rat liver transplantation. The donor rats in the heat preconditioning (HP) group were subjected to heat preconditioning 48 h before graft harvesting. The liver isografts were preserved in Euro-Collins solution for 8 h, and then transplanted orthotopically. The one-week survival rate of the HP group was significantly better than that of the control (C) group. The serum levels of interleukin-6 and interleukin-10 were significantly lower in the HP group than in the C group. Histological staining revealed that the stagnation of red blood cells and infiltration of neutrocytes were reduced in the HP group. The expression of intercellular adhesion molecule-1 was decreased around the central vein in the HP group, as revealed by immunohistochemistry. These results indicate that heat preconditioning downregulates cytokine release and reduces the frequency of microcirculation disorders.

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Expression of Intercellular Adhesion Molecule-1 (ICAM-1) During Ischemia–Reperfusion in Human Liver Tissue Allograft: Image Analysis by Confocal Laser Scanning Microscopy

Cited by 13 publications

References 36 publications

The Landscape of Digital Pathology in Transplantation: From the Beginning to the Virtual E-Slide

The Landscape of Digital Pathology in Transplantation: From the Beginning to the Virtual E-Slide

A mechanism for the anti-fibrogenic effects of the pregnane X receptor (PXR) in the liver: Inhibition of NF-κB?

Downregulation of Cytokine Release by Heat Preconditioning of Livers Used for Transplantation in Rats

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