Expression of Interferon‐tau mRNA in Bovine Embryos Derived from Different Procedures

N, Yao; Pc, Wan; Zd, Hao; Ff, Gao; Yang, Ling; Ms, Cui; Wu, Yiling; Jh, Liu; S, Liu; Chen, H; Sm, Zeng

doi:10.1111/j.1439-0531.2007.01009.x

Cited by 39 publications

(24 citation statements)

References 55 publications

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“…We saw no difference in SOX2, CDX2, and IFNT expression levels, which agreed with our previous results and those described by others Ross et al, 2009;Wang et al 2011;Yao et al, 2009). NANOG gene expression was slightly upregulated in SCNT groups versus IVF, as shown previously .…”

supporting

confidence: 95%

Effects of Donor Fibroblasts Expressing OCT4 on Bovine Embryos Generated by Somatic Cell Nuclear Transfer

Goissis

Suhr

Cibelli

2013

Cellular Reprogramming

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The production of healthy, live, cloned animals by somatic cell nuclear transfer (SCNT) has been hampered by low efficiencies. Significant epigenetic changes must take place to ensure proper chromatin remodeling in SCNT. We hypothesized that exogenous expression of OCT4 in donor fibroblasts prior to its fusion with enucleated oocytes would facilitate SCNT reprogramming. We infected bovine adult fibroblasts with retroviral vectors containing yellow fluorescent protein (YFP) only, or the OCT4 gene fused to YFP (YO). We found that development to the blastocyst stage was not different between NT-YFP and NT-YO groups. NT-YFP embryos had the fewest trophoblast cells, measured by numbers of CDX2-positive cells. Fibroblasts expressing OCT4 had reduced levels of histone 3 lysine 9 or 27 trimethylation (H3K9me3 and H3K27me3, respectively). NT-YO blastocysts displayed higher H3K9me3 levels than IVF and NT-YFP embryos; however, they did not have different H3K27me3 levels. Levels of XIST mRNA expression in NT-YO and NT-YF were higher when compared to in vitro-fertilized blastocysts. We observed no differences in the expression of SOX2, NANOG, and CDX2. Although overexpression of OCT4 in donor cells increased H3K9me3 and did not reduce XIST gene expression, we show that a single transcription factor can affect the number of trophectoderm cells in bovine SCNT embryos.

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supporting

confidence: 95%

Effects of Donor Fibroblasts Expressing OCT4 on Bovine Embryos Generated by Somatic Cell Nuclear Transfer

Goissis

Suhr

Cibelli

2013

Cellular Reprogramming

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“…However, another team reported no significant difference between IVF and parthenogenetic bovine embryos at Day 7 with semiquantitative RT-PCR. In this study, it appears that TP-1 mRNA was detected as early as the 16-cell stage for IVF embryos and at early blastocyst stage for PA embryos (Yao et al 2009). This finding also suggests that PA embryos are probably delayed in their development compared with their IVF counterparts.…”

Section: Discussionmentioning

confidence: 89%

Gene expression analysis of bovine blastocysts produced by parthenogenic activation or fertilisation

Labrecque

Sirard

2011

Reprod. Fertil. Dev.

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The processes underlying the very first moments of embryonic development are still not well characterised in mammals. To better define the kinetics of events taking place following fertilisation, it would be best to have perfect synchronisation of sperm entry. With fertilisation occurring during a time interval of 6 to 12h in the same group of fertilised oocytes, this causes a major variation in the time of activation of embryonic development. Bovine parthenogenesis could potentially result in better synchronisation and, if so, would offer a better model for studying developmental competence. In the present study, bovine oocytes were either parthenogenetically activated or fertilised and cultured in vitro for 7 days. Gene expression analysis for those two groups of embryos at early and expanded stages was performed with BlueChip, a customised 2000-cDNA array developed in our laboratory and enriched in clones from various stages of bovine embryo development. The microarray data analysis revealed that only a few genes were differentially expressed, showing the relative similarity between those two kinds of embryos. Nevertheless, the fact that we obtained a similar diversity of developmental stages with parthenotes suggests that synchronisation is more oocyte-specific than sperm entry-time related. We then analysed our data with Ingenuity pathway analysis. Networks of genes involved in blastocyst implantation but also previous stages of embryo development, like maternal-to-embryonic transition, were identified. This new information allows us to better understand the regulatory mechanisms of embryonic development associated with embryo status.

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“…The relative expression of POU5F1 transcript was quantified in in vitro‐cultured bovine embryos as a proxy to assess embryo quality (Niemann and Wrenzycki, ; Yao et al, ). POU5F1 expression was increased 0.87‐fold by the presence of L ‐arginine when compared with the control group cultured in the absence of L ‐arginine ( P < 0.05) (Fig.…”

Section: Resultsmentioning

confidence: 99%

Supplementation of bovine embryo culture medium with L‐arginine improves embryo quality via nitric oxide production

Santana

Silva

Costa

et al. 2014

Molecular Reproduction Devel

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Nitric oxide (NO) is a cell-signaling molecule that regulates a variety of molecular pathways. We investigated the role of NO during preimplantation embryonic development by blocking its production with an inhibitor or supplementing in vitro bovine embryo cultures with its natural precursor, L-arginine, over different periods. Endpoints evaluated included blastocyst rates, development kinetics, and embryo quality. Supplementation with the NO synthase inhibitor N-Nitro-L-arginine-methyl ester (L-NAME) from Days 1 to 8 of culture decreased blastocyst (P < 0.05) and hatching (P < 0.05) rates. When added from Days 1 to 8, 50 mM L-arginine decreased blastocyst rates (P < 0.001); in contrast, when added from Days 5 to 8, 1 mM L-arginine improved embryo hatching rates (P < 0.05) and quality (P < 0.05) as well as increased POU5F1 gene expression (P < 0.05) as compared to the untreated control. Moreover, NO levels in the medium during this culture period positively correlated with the increased embryo hatching rates and quality (P < 0.05). These data suggest exerts its positive effects during the transition from morula to blastocyst stage, and that supplementing the embryo culture medium with L-arginine favors preimplantation development of bovine embryos.

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Expression of Interferon‐tau mRNA in Bovine Embryos Derived from Different Procedures

Cited by 39 publications

References 55 publications

Effects of Donor Fibroblasts Expressing OCT4 on Bovine Embryos Generated by Somatic Cell Nuclear Transfer

Effects of Donor Fibroblasts Expressing OCT4 on Bovine Embryos Generated by Somatic Cell Nuclear Transfer

Gene expression analysis of bovine blastocysts produced by parthenogenic activation or fertilisation

Supplementation of bovine embryo culture medium with L‐arginine improves embryo quality via nitric oxide production

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