Expression of interleukin‐1α and interleukin‐1 receptor type I genes in murine brown adipose tissue

Burýšek, Ladislav; Tvrdí, Petr; Houštěk, J

doi:10.1016/0014-5793(93)81717-e

Cited by 14 publications

(6 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The role of adipose tissues in immune response and inflammation has been suggested by previous studies (Choy et al, 1992; White et al, 1992; Burysek et al, 1993; Cianflone and Maslowska, 1995; Peake et al, 1997; Pond, 1999) including our recent demonstration that preadipocytes present macrophage‐like functions (Cousin et al, 1999). The present work is the first evidence for a modification of these functions in preadipocytes under inflammatory stimuli such as thioglycollate treatment and/or pathological situation such as obesity.…”

Section: Discussionsupporting

confidence: 54%

Altered macrophage-like functions of preadipocytes in inflammation and genetic obesity

et al. 2001

View full text Add to dashboard Cite

We recently demonstrated that preadipocytes exhibit functional features of macrophages, such as phagocytosis and anti-microbial activity, suggesting that preadipose cells could play a role in the inflammatory process or immune response. The aim of this study was to compare these functions of both macrophages and cells from stroma-vascular fraction (SVF) of the adipose tissue in two different situations, obesity and inflammation, characterized by alterations in immune responsiveness. We demonstrated that ob/ob mice exhibited strong decrease in antimicrobial activity of both macrophages and SVF. This defect is compensated in SVF, at least in part, by an enhancement of phagocytosis that does not seem to be due to an increased macrophage number. In vitro leptin treatment of SVF and macrophages from obese mice did not restore their immune defects. Thioglycollate treatment of lean and obese mice induced an inflammatory process that led to an increase in macrophage activity in both strains. This stimulation also observed in SVF from lean mice is not present in obese ones. This work demonstrated that SVF immune functions could be modified in different pathological situations such as inflammation and obesity and sustained the new physiological role of preadipocytes in these processes.

show abstract

Section: Discussionsupporting

confidence: 54%

Altered macrophage-like functions of preadipocytes in inflammation and genetic obesity

et al. 2001

View full text Add to dashboard Cite

show abstract

“…Cultured cells were washed with PBS prior to total RNA isolation according to the guanidinium/phenol/chloroform procedure [12]. Northern blots (20 μg total RNA aliquots) were sequentially hybridized with cDNA probes radiolabeled with [α‐ 32 P]dCTP (3000 Ci/mmol) using the Random Priming Kit (Boehringer Mannheim) essentially as before [13]. Mouse IL‐1α cDNA was 1.6‐kbp fragment cut by Pst I and Eco RI from the pIL1301 plasmid provided by P.T.…”

Section: Methodsmentioning

confidence: 99%

“…Mouse IL‐6 cDNA (1.1‐kbp Eco RI fragment) was kindly provided by Van Snick (Ludwig Institute for Cancer Research, Brussels, Belgium) [15]. The probe for β‐actin was identical with that used earlier [13]. Radioactivity on the membranes was quantified by PhosphorImager analysis system (Molecular Dynamics).…”

Section: Methodsmentioning

confidence: 99%

β‐Adrenergic stimulation of interleukin‐1α and interleukin‐6 expression in mouse brown adipocytes

Burýšek

Houštěk

1997

FEBS Letters

View full text Add to dashboard Cite

Mouse brown adipocytes in primary culture were shown to contain high levels of mRNA for interleukin-la (IL-la) which could be further stimulated up to 9-fold by norepinephrine (NE). Even higher stimulation by NE, up to 40-fold, was found in case of interleukin-6 (IL-6). Time-course of activation of both genes was biphasic, but the response of IL-6 gene was slower than of IL-la gene. IL-la mRNA level reached the maximum after 1 h and the second, lower increase, occurred after 8 h. IL-6 mRNA level showed first maximum after 2 h, but the highest level was found after 8 h. Similarly to NE, the expression of ILla and IL-6 genes was stimulated by selective P-adrenergic agonist isoproterenol, p 3 -selective agonist CGP-12117, forskoline and db-cAMP. The activation of both genes by CGP-12177 was dose-dependent with the optimum at 100 nM concentration. Stimulation of oc-adrenergic receptors by cirazoline and oxymetazoline was without any effect. When the expression of IL-6 was studied at the protein level, the stimulation of IL-6 gene via Pareceptors resulted in secretion of IL-6 up to the concentration 10 ng/ml culture media in 24 h. The results indicate a new type of regulation of expression of IL-la and IL-6 genes in brown adipocytes by catecholamines acting via p 3 -adrenergic receptors. The resulting increase in IL-6 production by brown adipocytes could significantly contribute to systemic levels of IL-6.

show abstract

“…The intracellular pathway involved is unknown, but it is probable that the response is mediated by the IL‐1R1 receptor that is found in the tissue. The summary is based on data by Burysek et al 65–67…”

Section: Novel Scheme: More Than An Effector Organ?mentioning

confidence: 99%

Brown Adipose Tissue: More Than an Effector of Thermogenesis?^a

Cannon

Houštěk

Nedergaard

1998

Annals of the New York Academy of Sciences

115

View full text Add to dashboard Cite

Brown adipose tissue (BAT) produces heat by oxidation of fatty acids. This takes place when the tissue is stimulated by norepinephrine; the molecular background for the ability of BAT to produce heat is the tissue-specific mitochondrial protein UCP1. In the classic view of BAT with respect to fever, BAT is an effector organ, producing heat especially during the onset phase of the fever. There is good evidence that BAT thermogenesis is stimulated via a lipopolysaccharide (LPS), interleukin (IL)-1 beta, IL-6, prostaglandin E cascade. Under physiologic conditions of constantly stimulated activity, BAT is expected to be recruited, but in fevers this is only evident in thyroxine fever. However, BAT may be more than merely an effector. There are indications of a correlation between the amount of BAT and the intensity of fevers, and brown adipocytes can indeed produce IL-1 alpha and IL-6. Furthermore, brown adipocytes are directly sensitive to LPS; this LPS sensitivity is augmented in brown adipocytes from IL-1 beta-deficient mice. Thus, BAT may also have a controlling role in thermoregulation. The existence of transgenic mice with ablations of proteins central in fever and in BAT thermogenesis opens up possibilities for identification and elucidation of this putative new role for brown adipose tissue as an endocrine organ involved in the control of fever.

show abstract

Expression of interleukin‐1α and interleukin‐1 receptor type I genes in murine brown adipose tissue

Cited by 14 publications

References 31 publications

Altered macrophage-like functions of preadipocytes in inflammation and genetic obesity

Altered macrophage-like functions of preadipocytes in inflammation and genetic obesity

β‐Adrenergic stimulation of interleukin‐1α and interleukin‐6 expression in mouse brown adipocytes

Brown Adipose Tissue: More Than an Effector of Thermogenesis?^a

Contact Info

Product

Resources

About

Expression of interleukin‐1α and interleukin‐1 receptor type I genes in murine brown adipose tissue

Cited by 14 publications

References 31 publications

Altered macrophage-like functions of preadipocytes in inflammation and genetic obesity

Altered macrophage-like functions of preadipocytes in inflammation and genetic obesity

β‐Adrenergic stimulation of interleukin‐1α and interleukin‐6 expression in mouse brown adipocytes

Brown Adipose Tissue: More Than an Effector of Thermogenesis?a

Contact Info

Product

Resources

About

Brown Adipose Tissue: More Than an Effector of Thermogenesis?^a