Expression of Intermediate Filament Proteins and Neuronal Markers in the Human Fetal Gut

Rauch, Ulrich; Klotz, Markus; Maas-Omlor, Silke; Wink, Elvira; Hänsgen, Andrea; Hagl, Cornelia Irene; Holland‐Cunz, Stefan; Schäfer, Karl‐Herbert

doi:10.1369/jhc.4a6495.2005

Cited by 34 publications

(39 citation statements)

References 22 publications

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“…Nestin+ cells co-expressing either PGP 9.5 or S100 retain pluripotent characteristics and are competent to differentiate along the neuronal or glial lineages, respectively (Chalazonitis et al 1998;Rauch et al 2006b;Shi et al 2008). Decreasing nestin expression reflects neuronal and glial lineage progression and differentiation during development (Chalazonitis et al 1998;Rauch et al 2006b;Young et al 2003;Shi et al 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Progenitor cells that co-express nestin and the panneuronal marker protein gene product 9.5 (PGP 9.5) appear in the human fetal ENS at the 14th week of gestation and disappear by the 30th week (Rauch et al 2006b). In the normal adult human GI tract, nestin expression has been recognized in both glial cells and interstitial cells of Cajal of the myenteric plexus (Vanderwinden et al 2002), but nestin-positive (nestin+) neurons have not been identified in situ in human adult GI tract tissue.…”

Section: Introductionmentioning

confidence: 99%

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Evidence for neural progenitor cells in the human adult enteric nervous system

et al. 2011

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Putative neural stem cells have been identified within the enteric nervous system (ENS) of adult rodents and cultured from human myenteric plexus. We conducted studies to identify neural stem cells or progenitor cells within the submucosa of adult human ENS. Jejunum tissue was removed from adult human subjects undergoing gastric bypass surgery. The tissue was immunostained, and confocal images of ganglia in the submucosal plexus were collected to identify protein gene product 9.5 (PGP 9.5) - immunoractive neurons and neuronal progenitor cells that coexpress PGP 9.5 and nestin. In addition to PGP-9.5-positive/nestin-negative neuronal cells within ganglia, we observed two other types of cells: (1) cells in which PGP 9.5 and nestin were co-localized, (2) cells negative for both PGP 9.5 and nestin. These observations suggest that the latter two types of cells are related to a progenitor cell population and are consistent with the concept that the submucosa of human adult ENS contains stem cells capable of maintenance and repair within the peripheral nervous system.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evidence for neural progenitor cells in the human adult enteric nervous system

et al. 2011

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“…At present, nestin protein is generally used as a reliable biological marker of NPCs of CNS, to distinguish the ''ripe'' neural cells from NPCs in vitro and vivo (Lendahl, 1997;Yaworsky and Kappen, 1999;Aoki et al, 2000;Mitsuhashi et al, 2001;Englund et al, 2002;Schwartz et al, 2003;Takahashi et al, 2003;Pau and Wolf, 2004;Fujiwara et al, 2004;Wiese et al, 2004;Mazurová et al, 2006). However, nestin expression has also been reported outside of CNS, such as in the developing myotome (Zimmerman et al, 1994), skeletal muscle precursors (Lendahl et al, 1990;Zimmerman et al, 1994), mesenchymal pancreatic cells (Selander and Edlund, 2002), intestine (Rauch et al, 2006), and cranial ganglia (Elshamy and Ernfors, 1996). Because our study was focused on observing nestin expression in the mouse brain, we did not pay more attention to detecting whether nestin expression might be correlated with nestin expression of the other cells outside of the CNS reported in the previous research.…”

Section: Discussionmentioning

confidence: 99%

Description of distributed features of the nestin‐containing cells in brains of adult mice: A potential source of neural precursor cells

Tao

et al. 2009

J of Neuroscience Research

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The distribution of neural precursor cells (NPCs) in adult mice brain has so far not been described. Therefore, we investigated the distribution of NPCs by analyzing the nestin-containing cells (NCCs) in distinct brain regions of adult nestin second-intron enhancer-controlled LacZ reporter transgenic mice through LacZ staining. Results showed that NCCs existed in various regions of adult mouse brain. In cerebellum, the greatest number of NCCs existed in cortex of the simple lobule, followed by cortex of the cerebellar lobule. In olfactory bulb, NCCs were most numerous in the granular cell layer, followed by the mitral cell layer and the internal plexiform, glomerular, and external plexiform layers. In brain nuclei (nu), NCCs were most numerous in the marginal nu, followed by the brainstem and diencephalon nu. NCCs in sensory nu of brainstem were more numerous than in motor nu, and NCCs in the dorsal of sensory nu were more numerous than in the ventral part. In brain ventricle systems, NCCs were largely distributed in the center of and external to the lateral ventricle, the inferior part of the third ventricle, the dorsal and inferior parts of the fourth ventricle, and the gray matter around the cerebral aqueduct. NCCs in the left vs. right brain were not significantly different. These data collectively indicate that NCCs were extensively distributed in the cerebellum and olfactory bulb, the partial nu of the marginal system, the partial brain nu adjacent to brain ventricle systems, the subependymal zone, and the cerebral cortex around the marginal lobe and were a potential source of NPCs.

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“…In this double-transgenic mouse, all neural crest cell progeny are efficiently and stably marked, even in the postnatal animals (Chai et al 2000;Jiang et al 2000). In addition, because many markers that identify developing neural crest-derived cells in mouse embryos have been discovered to date (Sommer et al 1995;Kim et al 2003;Young et al 2003;Rauch et al 2006), we also analyzed mouse embryo immunoreactivity with the TuJ1, NF160, nestin, P75 NTR , and Sox10 antibodies. In the Cx43-lacZ embryos, the ectomesenchymal neural crest cells that were distributed in the pharyngeal arches were intensely immunoreactive for b-galactosidase.…”

Section: Discussionmentioning

confidence: 99%

Expression of the Epithelial Marker E-Cadherin by Thyroid C Cells and Their Precursors During Murine Development

Kameda

Nishimaki

Chisaka

et al. 2007

J Histochem Cytochem.

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S U M M A R Y Studies of chick-quail chimeras have reported that avian ultimobranchial C cells originate from the neural crest. It has consequently been assumed, without much supporting evidence, that mammalian thyroid C cells also originate from the neural crest. To test this notion, we employed both Connexin43-lacZ and Wnt1-Cre/R26R transgenic mice, because their neural crest cells can be marked. We also examined the immunohistochemical expression of a number of markers that identify migratory or postmigratory neural crest cells, namely, TuJ1, neurofilament 160, nestin, P75NTR, and Sox10. Moreover, we examined the expression of E-cadherin, an epithelial cell marker. At embryonic day (E)10.5, the neural crest cells densely populated the pharyngeal arches but were not distributed in the pharyngeal pouches, including the fourth pouch. At E11.5, the ultimobranchial rudiment formed from the fourth pouch and was located close to the fourth arch artery. At E13.0, this organ came into contact with the thyroid lobe, and at E13.5, it fused with this lobe. However, the ultimobranchial body was not colonized by neural crest-derived cells at any of these developmental stages. Instead, all ultimobranchial cells, as well as the epithelium of the fourth pharyngeal pouch, were intensely immunoreactive for E-cadherin. Furthermore, confocal microscopy of newborn mouse thyroid glands revealed colocalization of calcitonin and E-cadherin in the C cells. The cells, however, were not marked in the Wnt-Cre/R26R mice. These results indicated that murine thyroid C cells are derived from the endodermal epithelial cells of the fourth pharyngeal pouch and do not originate from neural crest cells.

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Expression of Intermediate Filament Proteins and Neuronal Markers in the Human Fetal Gut

Cited by 34 publications

References 22 publications

Evidence for neural progenitor cells in the human adult enteric nervous system

Evidence for neural progenitor cells in the human adult enteric nervous system

Description of distributed features of the nestin‐containing cells in brains of adult mice: A potential source of neural precursor cells

Expression of the Epithelial Marker E-Cadherin by Thyroid C Cells and Their Precursors During Murine Development

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