Expression of intracellular calcium-binding proteins in cultured skin fibroblasts from Alzheimer and normal aged donors

Föhr, Ursula G.; Gibson, Gary E.; Tofel-Grehl, Beth; Schäfer, Beat W.; Heizmann, Claus W.

doi:10.1016/0167-4889(94)90100-7

Cited by 9 publications

(3 citation statements)

References 51 publications

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“…However, there is substantive information to show the action of calcium on keratinocyte and fibroblast proliferation, migration, and functional morphogenesis 95,157 . Both cell types are actively involved in wound reconstruction at this time and are highly sensitive to extracellular calcium concentrations through cell surface receptors 96,158 . Keratinocytes remain in a proliferative and motile state at calcium concentrations at least 100 times lower than fibroblasts, but “excessive” calcium in the wound environment leading to a depression in proliferation and chemotactic responses is a cause of delayed healing 159 .…”

Section: The Inflammatory Phasementioning

confidence: 99%

Calcium: a potential central regulator in wound healing in the skin

Lansdown

2002

Wound Repair Regeneration

316

201

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Calcium has an established role in the normal homeostasis of mammalian skin and serves as a modulator in keratinocyte proliferation and differentiation. Gradients of calcium concentration increasing from 0.5 mM in the basal layer to > 1.4 mM in the stratum granulosum are consistent with migration patterns in response to minor abrasion (normal wear). Dermal fibroblasts require calcium but are approximately 100 times less sensitive than keratinocytes. Normal calcium metabolism in the skin is dependent on cell membrane and cytosolic calcium binding proteins (calmodulin, cadherins, etc.), but their modulation through parathyroid hormone, vitamin D or growth factors in normal or damaged tissue is not well documented. In wound repair, calcium is predominantly involved as Factor IV in the hemostatic phase, but it is expected to be required in epidermal cell migration and regeneration patterns in later stages of healing. Calcium alginate dressings are designed to liberate calcium early in the acute phase to promote hemostasis, but it is presently unclear whether the supplementary calcium influences the intracellular environment at later stages of wound repair, notably during the remodeling phase. Although experimental studies suggest that control of calcium is obligatory in wound management, we know very little as to how calcium in the wound bed is modulated through hormones, vitamin D, or various growth factors. Also, there is limited information as to how calcium released either from dressings, platelets, or from the circulation through the action of parathyroid hormone, growth factors or other modulators influences cell migration and remodeling in skin wounds, although experimental models suggest that management of calcium is essential in wound management.

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Section: The Inflammatory Phasementioning

confidence: 99%

Calcium: a potential central regulator in wound healing in the skin

Lansdown

2002

Wound Repair Regeneration

316

201

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“…These include 13-adrenergic stimulated formation of cyclic AMP Gibson 1993, Vestling et al, 1997), bradykinin stimulated formation of cyclic IP 3 (Huang et al, 1991(Huang et al, , 1993(Huang et al, , 1995, several aspects of calcium homeostasis (Gibson et al, 1996a,b;Ito et al, 1994;Fohr et al, 1994;Etcheberrigaray et al, 1993;Huang et al, 1991) and c~-ketoglutarate dehydrogenase activities (Sheu et al, 1994). The more standardized that the tissue culture methods are throughout the world, the better the chances that cultures can be used to clarify the molecular and cellular mechanisms in aging and disease of aging including Alzheimer's disease.…”

Section: Resultsmentioning

confidence: 96%

A reproducible procedure for primary culture and subsequent maintenance of multiple lines of human skin fibroblasts

Gibson

Tofel-Grehl

Scheffold

et al. 1998

AGE

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Cultured fibroblasts are a valuable tool to study many cellular processes and their modification by aging. Fibroblasts are a useful cell type in which to study many diseases, including those of the nervous system, in which a strong genetic component is suspected. Fibroblasts permit the study of multiple, dynamic processes in living cells, while avoiding the effect of the dying process and post-mortem artifacts that limit other approaches. For results to be comparable across time in one laboratory or consistent between laboratories, the detailed culture techniques require meticulous care and replicability. Lack of attention to detail in initial stages can lead to selection of different cell populations. Small variations in othe variables such as batches of serum can significantly alter growth rates and comparisons of cells from controls and Alzheimer patients. The aim of this paper is to present a detailed protocol for comparison of multiple cell lines from many patients. An example of using this approach to study growth and phase out (i.e., senescence) of cells from Alzheimer patients is presented. This procedure represents a modification of an earlier protocol (Cristofalo and Charpentier, 1980).

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“…The hypothesis that calcium-binding proteins are neuroprotective is supported by studies on different types of neurons in vivo (Heizmann and Braun 1992;Kotti et al 1996;Fohr et al 1994;Morris et al 1995) and in vitro (Lukas and Jones 1994;Mattson et al 1991;Mockel and Fisher 1994;Isaacs et al 1996;Pike and Cotman 1995;Abdel-Hamid and Baimbridge 1997). A calbindin containing subpopulation of neurons in the substantia nigra is spared in Parkinson's disease and in animal models of this disease (Iacopino et al 1992;Mouatt-Prigent et al 1994).…”

Section: Enrichment Of Calretinin-positive Cells By Medium Compositionmentioning

confidence: 96%

Enrichment of unipolar brush cell-like neurons in primary rat cerebellar cultures

Anelli

Mugnaini

2001

Anatomy and Embryology

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Unipolar brush cells are a distinct class of excitatory interneurons situated in the granular layer of the cerebellar cortex, where they form giant synapses with individual mossy fiber terminals. We have previously shown that primary cerebellar cell cultures from embryonic and postnatal rodents contain neurons displaying morphological and chemical phenotypes characteristic of unipolar brush cells in situ, including intense staining with calretinin antiserum. In cultures from both embryonic and postnatal rats, the large majority of calretinin-positive neurons are unipolar brush cells, while granule cells are usually calretinin-negative. A small percentage of putative Golgi/Lugaro cells also express calretinin. We demonstrate here that the developmental stage of the source tissue, the concentration of potassium in the medium, and treatment with glutamate after differentiation have substantial effects on the density of putative unipolar brush cells in the cultures. In dissociated cultures obtained from embryos at gestation day E18 and E20 and from pups at postnatal day P0, P2, P5, P8, and P10 grown in 25 mM KCl, the percentage calretinin-positive cells progressively decreases from 24% to 0.1% of total cells. In cultures from E20 embryos grown in physiological potassium (5 mM KCl), calretinin-positive cells are enriched to approximately 60% of total cells, while the majority of calretinin-negative cells die. In embryonic cultures exposed to high concentrations of glutamate after 12 days in vitro, calretinin-positive neurons have a survival advantage over calretinin-negative cells and represent up to 83% of total cells.

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Expression of intracellular calcium-binding proteins in cultured skin fibroblasts from Alzheimer and normal aged donors

Cited by 9 publications

References 51 publications

Calcium: a potential central regulator in wound healing in the skin

Calcium: a potential central regulator in wound healing in the skin

A reproducible procedure for primary culture and subsequent maintenance of multiple lines of human skin fibroblasts

Enrichment of unipolar brush cell-like neurons in primary rat cerebellar cultures

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