1993
DOI: 10.1182/blood.v82.4.1151.1151
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Expression of isoforms of the human receptor tyrosine kinase c-kit in leukemic cell lines and acute myeloid leukemia

Abstract: Because mutations in receptor tyrosine kinases may contribute to cellular transformation, studies were undertaken to examine c-kit in human leukemia. Isoforms of c-kit have been characterized in the human megakaryoblastic leukemia cell line M-07. Deletion of the four amino acids Gly-Asn-Asn-Lys in the extracellular domain represents an alternatively spliced isoform that has been shown by others, in mice, to be associated with constitutive receptor autophosphorylation (Reith et al, EMBO J 10:2451, 1991). Additi… Show more

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Cited by 104 publications
(22 citation statements)
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“…This results from an alternative 3' splice acceptor site usage. 45 The two iso-forms S715(À) and S715(+) are co-expressed in similar quantities in patients with MCAD and in healthy controls ( Figure 1). 25,26 This suggests that these isoforms have no substantial involvement in the induction of a pathological increase in mast cell activity.…”
Section: Genetic Alterations In Patients With Systemic Mcadmentioning
confidence: 88%
“…This results from an alternative 3' splice acceptor site usage. 45 The two iso-forms S715(À) and S715(+) are co-expressed in similar quantities in patients with MCAD and in healthy controls ( Figure 1). 25,26 This suggests that these isoforms have no substantial involvement in the induction of a pathological increase in mast cell activity.…”
Section: Genetic Alterations In Patients With Systemic Mcadmentioning
confidence: 88%
“…Several single nucleotide polymorphisms (SNP) in KIT and PDGFRA coding sequences (SNP database at http://www.ncbi.nlm.nih.gov) and two alternative splicing sites in KIT have been reported 175,176 . KIT and PDGFRA polymorphisms and alternatively spliced variants of KIT mRNA should not be confused with activating oncogenic KIT mutations 177 …”
Section: Technical Considerationsmentioning
confidence: 99%
“…29,30 The selected C1642 clone pRUFneo(M541L KIT) encoded the GNNK-isoform which is the predominant form in human cells. 31 Plasmid used for transfection was purified using a Qiagen plasmid kit (Qiagen, Hamburg, Germany). Table 1 Primer sets and conditions for polymerase chain reaction (PCR) amplification and denaturing high pressure liquid chromatography elution of human KIT exons.…”
Section: Expression Of C-kit M541l In Murine Factor-dependent Cellsmentioning
confidence: 99%