Expression of keratinocyte growth factor and its receptor in colorectal cancer

Jm, Otte; Schmitz, Frank; Banasiewicz, Tomasz; Drews, M.R.; Ur, Fölsch; Kh, Herzig

doi:10.1046/j.1365-2362.2000.00617.x

Cited by 21 publications

(20 citation statements)

References 32 publications

(39 reference statements)

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“…KGF fails to effectively stimulate the growth of two of three pancreatic cancer cell lines and of colorectal cancer cell lines, established from undifferentiated cancer or from a metastatic nodule (13,15). KGF-transfected prostate epithelial cells are not tumorigenic in nude mice (20).…”

Section: Introductionmentioning

confidence: 95%

“…KGFR is expressed in epithelial cells throughout the gastrointestinal (GI) tract, and an intraperitoneal injection of KGF enhances normal intestinal epithelial cell growth (9,10). In the early stage of esophageal ulcer healing, the expression levels of Expression and roles of keratinocyte growth factor and its receptor in esophageal cancer cells (12)(13)(14). Pancreatic cancer cell line growth is stimulated by rhKGF administration (15).…”

Section: Introductionmentioning

confidence: 99%

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Expression and roles of keratinocyte growth factor and its receptor in esophageal cancer cells

Yoshino

Ishiwata²,

Watanabe³

et al. 2007

Int J Oncol

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Abstract. The keratinocyte growth factor receptor (KGFR), also known as FGFR2 IIIb, is mainly localized in epithelial cells and is activated by the keratinocyte growth factor (KGF) that is predominantly synthesized by mesenchymal cells. In this study, we examined the roles of KGFR and KGF in human esophageal cancer (EC). In noncancerous esophageal tissues, KGFR was localized in epithelial cells from the basal region of the epithelium to the lower one-third of the epithelium, and KGF was weakly localized in the basal to parabasal epithelial cells. On the other hand, Ki-67 was localized in the parabasal cells. In EC tissues, KGFR and KGF were expressed in cancer cells in 22 and 37 of 54 patients, respectively. The coexpression of KGFR and KGF in cancer cells was detected in 14 of 54 (26%) patients. Clinicopathologically, KGFR expression correlated with the well-differentiated cell type of EC (p<0.001), and KGF expression correlated with lymphatic invasion and lymph node metastasis (p=0.004 and 0.021, respectively). The coexpression of KGFR and KGF in cancer cells correlated with the well-differentiated cell type of EC (p=0.001). KGFR-positive, KGF-positive and KGFR/KGF coexpression patients tended to have shorter survival rates, but the survival rates were not statistically significantly different (p=0.44, 0.059 and 0.112, respectively). In human EC cell lines (TE-1, TE-8 and TE-11), KGFR mRNA was expressed but no KGF mRNA was detected. The KGFR mRNA level was highest in TE-1 cells, derived from well-differentiated SCC and lowest in TE-8 cells. KGFR was detected in the cancer cell lines by Western blot analysis. Recombinant human KGF significantly stimulated the growth of TE-8 and -11 cells, derived from moderately differentiated SCC, but had no effect on TE-1 cell growth. These results suggest that KGFR expression correlates with the differentiation of a normal esophageal epithelium and the well-differentiated cell type of EC. On the other hand, KGF may induce the growth of some EC cells in a paracrine manner and closely correlates with lymphatic invasion and lymph node metastasis.

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Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Expression and roles of keratinocyte growth factor and its receptor in esophageal cancer cells

Yoshino

Ishiwata²,

Watanabe³

et al. 2007

Int J Oncol

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“…KGFR is expressed in epithelial cells throughout the gastrointestinal tract, and an intraperitoneal injection of KGF enhances normal intestinal epithelial growth (13,14). Regarding cancerous tissue, KGFR expression was previously reported in pancreatic cancer and colorectal cancer cells (15,16). Furthermore, pancreatic cancer cell line growth was stimulated by recombinant KGF administration (17), and dose-dependent cell growth by KGF was observed in welldifferentiated colorectal cancer cell lines (18).…”

Section: Introductionmentioning

confidence: 88%

Expression of keratinocyte growth factor receptor correlates with expansive growth and early stage of gastric cancer

Matsunobu

Ishiwata²,

Yoshino³

et al. 2006

Int J Oncol

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Abstract. The keratinocyte growth factor receptor, also known as KGFR/FGFR2 IIIb, is mainly localized in epithelial cells, and participates in the proliferation of these cells. In contrast, a recent study has revealed that the overexpression of KGFR in salivary adenocarcinoma induces growth inhibition, cell differentiation and apoptosis. We attempted to clarify the expression and role of KGFR in normal and cancerous human gastric tissues and cancer cell lines. Reverse-transcription polymerase chain reaction and Western blot analyses showed KGFR mRNA and its protein expression in NUGC-4, KATO-III and MKN-7 gastric cancer cell lines, but not in the NS-8 cell line. Immunohistochemically, KGFR immunoreactivity was weakly detected in the luminal surface of normal gastric epithelial cells. In addition, KGFR immunoreactivity was strongly detected in the nucleus and cytoplasm of many parietal cells. In gastric cancer tissue, KGFR was expressed in the cell membrane and cytoplasm of cancer cells in 46 of 126 (36.5%) cases. KGFR expression in gastric cancer cells was significantly associated with early-type macroscopic findings, shallow invasion of the gastric wall and expansive growth type. KGFR expression tended to correlate with a good prognosis in gastric cancer. These findings indicate that KGFR expression plays important roles in the differentiation of normal gastric epithelial cells and parietal cell functions. Furthermore, a decreased expression level or the non-expression of KGFR in gastric cancer cells may be associated with the proliferation and invasion of gastric cancer cells and a poor prognosis for the patient.

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“…Все четыре наиболее изученных рецепто-ра к фактору роста фибробластов экспресси-руются клетками рака толстой кишки [92,93]. FGFR1 и FGFR2 экспрессированы как в адено-мах толстой кишки, так и в клетках инвазивно-го рака, что может указывать на участие FGFRs в процессах трансформации неизмененного эпителия в опухоль [94].…”

Section: рак толстой кишкиunclassified

“…Рассматривая клинико-морфологические особенности опухоли при гиперэкспрессии FGFR2, отметим высокую степень дифференци-ровки таких опухолей [92,93]. Нейроэндокрин-ные клетки мироокружения опухоли способны экскретировать FGF7 -лиганд изоформы ре-цептора FGFR2 IIIВ [98].…”

Section: Wwwmalignanttumoursorg Malignant Tumoursunclassified