Expression of keratins during experimentally induced carcinogenesis in hamster cheek pouch visualized polyclonal and monoclonal antibodies

Abstract

We obtained immunohistochemical profiles of several keratin proteins during experimentally induced carcinogenesis in hamster cheek-pouch mucosa using a polyclonal antibody (TK; detecting keratins with molecular masses of 41-65 kilodalton) and two monoclonal antibodies (KL1, 55- to 57-kilodalton keratins; PKK1; 40-, 45- and 52.5-kilodalton keratins). The squamous epithelium of normal pouch mucosa exhibited positive TK staining in all layers, KL1 staining in the spinous layer and PKK1 staining in the basal layer, thus indicating a regional or zonal distribution pattern. Epithelia undergoing basal hyperplasia showed irregular localization of PKK1 binding, while hyperkeratinized lesions exhibited the binding pattern found in normal epithelium. In case of epithelial dysplasia, there was reduced KL1 staining in spinous cells and decreased PKK1 staining in the basal and parabasal layers. Papillomas exhibited a rather zonal distribution of keratin staining. All squamous-cell carcinomas, irrespective of their degree of keratinization and infiltration pattern, showed slight or no PKK1 staining. Such lesions were only positive for KL1-detectable keratins in keratinizing tumour cells and exhibited an irregular distribution of TK binding. The expression of keratin proteins during carcinogenesis in hamster cheek-pouch mucosa may parallel that of keratins in human squamous-cell carcinomas originating in the oral mucosa.

Lectin binding patterns in squamous epithelium in experimentally induced hamster buccal pouch carcinoma

“…Studies have been done to identify different cellular products comparing normal and neoplastic cells by using immunohistochemical techniques (26), However, very few studies have been focused on time related changes of neoplastic transformation during experimentally induced carcinogenesis in animals especially in the hamster cheek pouch (27,28), This is the first report to identify alterations in the levels and -.MSl^l^% distribution of sugar residues during the process of carcinogenesis in the mucosa of a hamster buccal pouch.…”

Immunohistochemical evaluation of keratin and involucrin distribution in Bowen's disease.

“…However, involucrin staining was negative throughout all strata (Fig. 1H) filaments and are useful markers for epithelial cells and lesions (1,6,7,9,10,17,18,23). Involucrin is a marker for squamous differentiation and terminal differentiation of squamous epithelia (4,8,13,16,21,22).…”

7,12‐Dimethylbenz[a]anthracene‐induced ‘early’ squamous cell carcinoma in the Golden Syrian hamster: evaluation of an animal model and comparison with ‘early’ forms of human squamous cell carcinoma in the upper aero‐digestive tract

“…Histopathologic findings were compared with premalignant and malignant changes in human carcinogenesis of the upper digestive tract. Immunohistochemical techniques were used to detect alterations of different keratin types in the carcinogenesis of the hamster cheek pouch and compared to the keratin patterns observed in the human carcinogenesis of the upper digestive tract and oesophagus (Hurlimann & Gardiol 1989;Gimenez-Conti 1990;Shuler et al 1987;Tatemoto et al 1987;Murase et al 1986). Samples of the hamster buccal pouch mucosae were formalin fixed, paraffin embedded, and sectioned in 5-m thick slices which were stained using standard immunoperoxidase techniques.…”