Expression of key genes in the caecum in CM5 and CM9 chickens of the meat cross «Smena 9» against the background of the replacement of fishmeal

Abstract

Relevance. Dependence on fishmeal hinders the development of the poultry industry. The aim of the study was to analyze the expression of key genes in the parent stock of Gallus gallus L. lines CM5 and CM9 of the meat cross «Smena 9» on the background of diets with the replacement of fish meal.Methods. Experiments were carried out on CM5 and CM9 chickens: groups 1A and 1B received the main diet (MD), 2A and 2B — MD with the replacement of fish meal with soy products, 3A and 3B — MD with the replacement of fish meal with sunflower products, 4A and 4B — MD with the replacement of fishmeal with soybean and sunflower products at a ratio of 50:50. Gene expression analysis was performed using quantitative reverse transcription PCR.Results. The most pronounced changes in the levels of expression of key genes against the background of the replacement of fishmeal with soybean and sunflower processed products concerned the maternal line Plymouth Rock CM9 compared to the paternal line Cornish CM5. Thus, the expression of SOD mRNA in groups 2B, 3B and 4B decreased by 14.3–100 times compared with control 1B (р ≤ 0.05). Whereas in the experiment on the CM5 line, the expression of the SOD gene in the experimental groups 2A and 3A decreased by no more than 3.5 times compared with the control 1A (р ≤ 0.05). In all experimental groups, zootechnical indicators of meat and egg productivity were noted at the level of control groups (р > 0.05).